Intrauterine Oxygen Tension in the Rat During Progestation: Its Possible Relation to Carbohydrate Metabolism and the Regulation of Nidation

Yochim, J. M.; Mitchell, J. A.

doi:10.1210/endo-83-4-706

Cited by 50 publications

(16 citation statements)

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“…These results are consistent with those reported for early embryonic development in mice [7], rabbits [8], swine [9], sheep [10,11], and cattle [10,12]. Five percent 02 closely mimics the 02 concentration in the oviducts of rats [25], rabbits [2,4], and monkeys [4]. Other studies did not consistently demonstrate beneficial effects of reduced 02 concentration on embryonic development for sheep [26], domestic cats [27], and cattle [21,22].…”

supporting

confidence: 87%

Development of Bovine Embryos in KSOM with Added Superoxide Dismutase And Taurine and with Five and Twenty Percent 021

Liu

Foote

1995

Biology of Reproduction

108

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To further define requirements for embryo development without the assistance of complex media, serum, or coculture, the effects of 5% and 20% O2 concentrations, superoxide dismutase (SOD) (experiment 1), and taurine (experiment 2) were tested in a single culture medium (KSOM modified with 2.5 mM HEPES). Bovine embryos were produced in vitro maturation (IVM) and in vitro fertilization (IVF) of oocytes. After fertilization, embryos were cultured in 5% O2:5% CO2:90% N2 at 30 degrees C for the first 40-44 h; then embryos with 4 to 8 cells were cultured in different treatments for another 6 days. Percentage development subsequently was based on this number. In experiment 1, more blastocysts were produced in 5% O2:5% CO2:90% N2 (37%) than in 20% O2:5% CO2:75% N2 (18%; p < 0.01). Blastocyst development with 0, 300, and 600 U/ml of SOD, respectively, was 26%, 26%, and 30% (p > 0.05). In experiment 2, more blastocysts were formed in 5% O2 (39%) than in 95% air (30%; p < 0.05). Also, with 20% O2 and 0, 7, and 14 mM taurine in the medium, blastocyst formation was 18%, 35%, and 36% (p < 0.05). However, in 5% O2, 7 and 14 mM taurine was not beneficial (p = 0.99), as 39% blastocysts were formed with no taurine. In conclusion, results from both experiments demonstrated that 5% O2 was superior to 20% O2 for development of IVM/IVF bovine embryos. SOD had no effect in either 5% or 20% O2. However, 7 mM and 14 mM taurine counteracted the negative effect of 20% O2.(ABSTRACT TRUNCATED AT 250 WORDS)

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confidence: 87%

Development of Bovine Embryos in KSOM with Added Superoxide Dismutase And Taurine and with Five and Twenty Percent 021

Liu

Foote

1995

Biology of Reproduction

108

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“…These enzymes have been investigated in the rat by both histochemical and biochemical techniques, particularly by Christie (1966Christie ( , 1967 and Yochim and his co-workers (Yochim & Mitchell, 1968;Clark & Yochim, 1971;Yochim, 1971;Yochim & Clark, 1971a, (Pearse, 1968).…”

mentioning

confidence: 99%

Lactic Dehydrogenase and Other Enzymes in the Mouse Uterus During the Periimplantation Period of Pregnancy

Hall¹

1973

Reproduction

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“…These metabolic adaptations may further be explained on the basis of 'intrauterine oxygen tension' which plays a vital role in regulation of LDH-subunit synthesis {Dawson et al 1964;Yochim and Mitchell 1968;Clark and Yochim 1971). The rise in uterine H/ M ratio during protein malnutrition could be due to increased oxygen availability (hence decreased contractile activity).…”

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confidence: 99%

Adaptive Changes in Uterine Distribution of Lactate Dehydrogenase Isoenzymes During Protein Malnutrition and Steroid Maintained Pregnancy in Rats

Rk¹,

Sk²

1980

Horm Metab Res

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The electrophoretic studies revealed that in non-pregnant and pregnant rats fed with protein-free diet (PFD), a significant increase in percentage of LDH1 and LDH2 (H-isozymes) was observed with a concomitant decrease in concentration of LDH4 and LDH5 (M-isozymes). The changes in LDH3 were not significant. Further, during protein deficiency heart-type activity (H-subunits) was increased markedly with a parallel decline in muscle-type activity; thus resulting in an increase in H/M ratio or analogue ratio (A.R.). However, the decrease in A.R. (below 2.00), observed during normal and steroid maintained pregnancy was due to higher muscle-type than heart-type activity. These observations suggest that protein malnutrition in rats favours a metabolic shift from anaerobic state to aerobic predominance in the uterus. The physiological significance of these adaptations has been discussed.

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Intrauterine Oxygen Tension in the Rat During Progestation: Its Possible Relation to Carbohydrate Metabolism and the Regulation of Nidation

Cited by 50 publications

References 0 publications

Development of Bovine Embryos in KSOM with Added Superoxide Dismutase And Taurine and with Five and Twenty Percent 021

Development of Bovine Embryos in KSOM with Added Superoxide Dismutase And Taurine and with Five and Twenty Percent 021

Lactic Dehydrogenase and Other Enzymes in the Mouse Uterus During the Periimplantation Period of Pregnancy

Adaptive Changes in Uterine Distribution of Lactate Dehydrogenase Isoenzymes During Protein Malnutrition and Steroid Maintained Pregnancy in Rats

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