Lactic Dehydrogenase and Other Enzymes in the Mouse Uterus During the Periimplantation Period of Pregnancy

Hall, Kathleen

doi:10.1530/jrf.0.0340079

Cited by 6 publications

(3 citation statements)

References 13 publications

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“…Although in situ hybridization was not a part of this study, histochemical methods localized the increase of 5'-NT activity to the primary decidua, a structure which develops soon after blastocyst implantation (Stewart and Peel, 1978;Parr and Parr, 1989). This pattern confirms other rodent studies showing intense uterine AMPase reaction product approximate to the conceptus (Christie, 1966;Hall, 1971Hall, , 1973Witte et al, 1991); however, those studies were inconclusive as to the specificity of the AMPase reac- HPLC-fluorescence chromatographic profile of embryodecidual units extracted on day 5 of gestation. The profile in (A) contains a large peak that comigrated with authentic E-ethenoadenosine (arrow); the profile in (B) is an aliquot of the same extract digested with ADA (37"C, 30 min) prior to derivatization.…”

Section: Discussionsupporting

confidence: 89%

“…5C). To verify that the levamisole-sensitive signal was due to nonspecific phosphatase activity, a nonnucleotide monophosphate, P-glycerophosphate, was substituted for AMP in the reaction cocktail (Hall, 1971(Hall, , 1973. A localized signal appeared in the secondary decidua (compare Fig.…”

Section: '-Nt Activity Is Localized To the Primary Decidua Whereas Amentioning

confidence: 99%

“…Detailed analysis of the cellular localization of ADA has recently been published (Hong et al, 1991;Knudsen et al, 1991;Witte et al, 1991). Previous studies on 5'-NT have not entailed analysis of the full postimplantation period nor have they resolved 5'-NT activity from that of nonspecific phosphatase reactions (Christie, 1966;Hall, 1971Hall, , 1973Witte et al, 1991). To localize 5'-NT activity, the present study used a wellestablished histochemical procedure (Scott, 1965) modified by the addition of levamisole to inhibit alkaline phosphatase (AP) activity (Pollard et al, 1990).…”

Section: '-Nt Activity Is Localized To the Primary Decidua Whereas Amentioning

confidence: 99%

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Adenosine levels in the postimplantation mouse uterus: Quantitation by HPLC‐fluorometric detection and spatiotemporal regulation by 5′‐nucleotidase and adenosine deaminase

Blackburn

Gao

Airhart

et al. 1992

Developmental Dynamics

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Extracellular adenosine has the potential to influence many aspects of target cell metabolism. The present study has determined the endogenous levels of adenosine in the pregnant mouse uterus and developing embryodecidual unit with respect to the expression of two key enzymes of adenosine metabolism, 5'-nucleotidase (5'-NT; EC 3.1.3.5) and adenosine deaminase (ADA; EC 3.5.4.4). To measure adenosine levels, nucleoside extracts were etheno-derivatized and quantitated by high-performance liquid chromatography-fluorescence detection (0.03 pmollmg protein sensitivity). Adenosine levels were determined to be 0.18 nmol/mg protein in the nonpregnant uterus; however, two statistically significant changes were identified in the pregnant uterus: (1) a periimplantation surge between day 3 (0.24 nmoVmg protein) and day 5 (0.59 nmoYmg protein) of gestation (plug day 0; implantation day 4); and (2) an early postimplantation decline between day 6 (0.54 nmollmg protein) and day 7 (0.10 nmollmg protein). The periimplantation adenosine surge coincided with uterine expression of 5'-NT, an enzyme which catalyzes the irreversible dephosphorylation of 5'-AMP to adenosine. 5'-NT expression was shown by Northern blot analysis to peak in the embryo-decidual unit on day 5 of gestation and then to decline through day 9; transcripts remained elevated in the placenta between day 9 and day 13 (the latest day examined in this study). By use of specific enzyme histochemistry, most 5'-NT activity was localized to the primary decidual :zone on day 5. This expression subsequently declined during regression of the primary decidua; however, 5'-NT appeared on giant trophoblast ((days 7-13) and the metrial gland (days 11-13).Other purine catabolic enzymes degrading AMP (adenylate deaminase) or generating adenosine (S-adenosylhomocysteine hydrolase) were not detected in the embryo-decidual unit suggesting that the net flux of utero-placental AMP catabolism proceeds with adenosine as an intermediate, this being the major pathway of adenosine formation.The sharp drop in adenosine levels between day 6 and day 7 coincided with a rise in the activity and mRNA expression of ADA, an enzyme which catalyzes the irreversible deamination of adenosine to inosine. ADA was previously localized to the secondary decidual zone (days 6 1 l), secondary giant cells (days 7-13), and spongiotrophoblasts (days 8-13) in the mouse (Knudsen et al., 1991). Results of developmental Northern blot analysis demonstrated a direct correlation of relative 5'-NT/ADA mRNA band intensity to adenosine content between day 4 and day 9 of gestation, suggesting that the local availability of adenosine in the antimesometrium is dependent upon the distribution of these enzymatic activities. Purine nucleoside phosphorylase and xanthine oxidase, which are two catabolic enzymes acting subsequent to 5'-NT and ADA in the sequential degradation of AMP to xanthine, remained low and constant in the tissues examined suggesting that the catabolic pathway is geared toward regulation of adenosine le...

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Section: Discussionsupporting

confidence: 89%

Section: '-Nt Activity Is Localized To the Primary Decidua Whereas Amentioning

confidence: 99%

Section: '-Nt Activity Is Localized To the Primary Decidua Whereas Amentioning

confidence: 99%

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Adenosine levels in the postimplantation mouse uterus: Quantitation by HPLC‐fluorometric detection and spatiotemporal regulation by 5′‐nucleotidase and adenosine deaminase

Blackburn

Gao

Airhart

et al. 1992

Developmental Dynamics

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The survival and implantation of mouse blastocysts at varying degrees of reduced atmospheric pressure

Blackburn

Clegg

1979

Teratology

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Pregnant mice were exposed to reduced atmospheric pressures ranging from 630 to 390 mm Hg during the pre-implantation and implantation periods and the numbers of embryos surviving 85 hours post coitum compared with those in litter-mate controls. Even at a pressure of 630 mm Hg (= 1,550 mm Hg) there was a significant fall in numbers of normal blastocysts and rise in abnormal forms before implantation, and implantation sites were reduced in number. The numbers of abnormal forms increased and implantation sites decreased at lower pressures, suggesting strongly that the hypoxia of reduce atmospheric pressure was responsible for the abnormalities observed. The pre-implantation period appears to be one during which the fertilised ovum is at particular risk, both of hypoxic damage and of failure to implant. Implantation may afford a degree of protection against hypoxia.

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McComb

Bowers

Posen

1979

Alkaline Phosphatase

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Lactic Dehydrogenase and Other Enzymes in the Mouse Uterus During the Periimplantation Period of Pregnancy

Abstract: Summary. A histochemical investigation of enzyme activities in the uterine endometrium of mice from Days 4 to 8 of the first pregnancy is described, Day 1 being the day on which a copulation plug appeared in the vagina. The

Cited by 6 publications

References 13 publications

Adenosine levels in the postimplantation mouse uterus: Quantitation by HPLC‐fluorometric detection and spatiotemporal regulation by 5′‐nucleotidase and adenosine deaminase

Adenosine levels in the postimplantation mouse uterus: Quantitation by HPLC‐fluorometric detection and spatiotemporal regulation by 5′‐nucleotidase and adenosine deaminase

The survival and implantation of mouse blastocysts at varying degrees of reduced atmospheric pressure

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