Intravaginal administration of lactic acid bacteria modulated the incidence of purulent vaginal discharges, plasma haptoglobin concentrations, and milk production in dairy cows

Ametaj, Burim N.; Iqbal, Summera; Selami, F.; Odhiambo, John F.; Wang, Y.; Gänzle, Michael G.; Dunn, Suzanna M.; Zebeli, Qendrim

doi:10.1016/j.rvsc.2014.02.007

Cited by 37 publications

(60 citation statements)

References 17 publications

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“…Moreover, elevated plasma NEFA have been associated with greater risk of metritis and the incidence of other periparturient diseases (Sordillo et al, 2009;Leblanc, 2010;Giuliodori et al, 2013). Because probiotics in our experiment were infused in the vaginal tract and not orally, the likely reason for lower plasma NEFA is that LAB lowered the incidence of uterine infections and boosted local production of secretory IgA in the vaginal mucus (Ametaj et al, 2014;Deng et al, 2015). Lactobacilli are known for their ability to prevent infections caused by pathogenic bacteria (Reid and Bruce, 2003;Doron and Gorbach, 2006).…”

Section: Discussionmentioning

confidence: 96%

“…Based on the data from a pilot study previously conducted by our group (Ametaj et al, 2014), 100 dairy cows in total were enrolled in this study in order to acquire a power of 85% to detect treatment differences in uterine infections and 10 samples from each group to detect treatment differences in blood variables, acute phase proteins, and IgG in the milk. Considering the incidence rate of mastitis is approximately 50%, 20 milk samples for each group were used for the composition assay.…”

Section: Laboratory Analysesmentioning

confidence: 99%

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Intravaginal probiotics modulated metabolic status and improved milk production and composition of transition dairy cows1

Deng

Odhiambo

Farooq

et al. 2016

Journal of Animal Science

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The objective of this investigation was to evaluate whether intravaginal infusion of probiotics (a lactic acid bacteria cocktail) around parturition would influence metabolic status and increase milk production of transition dairy cows. One hundred pregnant Holstein dairy cows were assigned to 1 of the 3 experimental groups receiving intravaginal infusion of probiotics or carrier (i.e., sterile skim milk) once a week at wk -2, -1, and +1 relative to calving as follows: 2 consecutive probiotics before parturition and 1 carrier dose after parturition (TRT1), 3 consecutive probiotics doses around parturition (TRT2), and 3 consecutive carrier doses around parturition (CTR). The probiotics were a lyophilized culture mixture composed of FUA3089 and FUA3138 and FUA3140 with a cell count of 10 to 10 cfu/dose. Blood was sampled from wk -2 to +3 and milk was sampled on the third day in milk (DIM) and from wk +1 to +5 on a weekly basis. Feed intake and milk production was monitored until wk +8. Results showed that the TRT2 group (366.12 ± 49.77 μmol/L) had a lower ( = 0.01) concentration of NEFA in the serum than the CTR group (550.85 ± 47.16 μmol/L). The concentrations of IgG in the milk were 32.71 ± 3.00 mg/mL in the TRT1 group, 17.47 ± 4.54 mg/mL in the TRT2 group, and 6.73 ± 3.43 mg/mL in the CTR group at 3 DIM ( < 0.01). Meanwhile, both the TRT1 and the TRT2 group had lower haptoglobin in the milk compared with the CTR group at 3 DIM ( < 0.01). The TRT1 group had greater milk protein content than the CTR group (2.99 ± 0.04 vs. 2.82 ± 0.04%; = 0.02), whereas the TRT2 group tended to have greater lactose content compared with the CTR group (4.53 ± 0.03 vs. 4.44 ± 0.03%; = 0.05). The effect of treatment interacted with parity with regards to milk production and feed efficiency. Multiparous cows in the TRT1 and TRT2 groups had greater milk production and feed efficiency than those in the CTR group ( < 0.01 and = 0.02, respectively). Among primiparous cows, those in the TRT2 group had greater milk production ( = 0.04) whereas those in the TRT1 group had lower feed intake ( < 0.01) than those in the CTR group. Both the TRT1 and the TRT2 groups had enhanced feed efficiency compared with the CTR group ( < 0.01). In conclusion, intravaginal infusion of lactic acid bacteria modulated concentrations of selected serum metabolites and milk components and increased milk efficiency of transition dairy cows.

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Section: Discussionmentioning

confidence: 96%

Section: Laboratory Analysesmentioning

confidence: 99%

Intravaginal probiotics modulated metabolic status and improved milk production and composition of transition dairy cows1

Deng

Odhiambo

Farooq

et al. 2016

Journal of Animal Science

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“…Because the traditional antimicrobial treatment applied for metritis may lack efficacy and does not typically improve reproductive performance of the treated animals (Gilbert and Schwark, 1992;Otero et al, 2006), the use of probiotic strains of bacteria has been proposed as an alternative to prevent postpartum uterine infections and inflammation (Ametaj et al, 2014;Deng et al, 2015). In our recent work, various individual strains of LAB were shown to protect against E. coli infection in vitro and reduce inflammation in epithelial cells (Genís et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

A combination of lactic acid bacteria regulates Escherichia coli infection and inflammation of the bovine endometrium

Genís

Sánchez-Chardi²,

Bach

et al. 2017

Journal of Dairy Science

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Uterine function in cattle is compromised by bacterial contamination and inflammation after calving. The objective of this study was to select a combination of lactic acid bacteria (LAB) to decrease endometrium inflammation and Escherichia coli infection. Primary endometrial epithelial cells were cultured in vitro to select the most favorable LAB combination modulating basal tissue inflammation and E. coli infection. Supernatants were obtained to determine expression of pro-inflammatory cytokines, and E. coli infection was evaluated after harvesting the tissue and plate counting. The selected LAB combination was tested in uterus explants to assess its capacity to modulate basal and acute inflammation (associated with E. coli infection). The combination of Lactobacillus rhamnosus, Pediococcus acidilactici, and Lactobacillus reuteri at a ratio of 25:25:2, respectively, reduced E. coli infection in vitro with (89.77%) or without basal tissue inflammation (95.10%) compared with single LAB strains. Lactic acid bacteria treatment reduced CXCL8 and IL1B expression 4.7- and 2.2-fold, respectively, under acute inflammation. Ex vivo, the tested LAB combination reduced acute inflammation under E. coli infection, decreasing IL-8, IL-1β, and IL-6 up to 2.2-, 2.5-, and 2.2-fold, respectively. In the total inflammation model, the LAB combination decreased IL-8 1.6-fold and IL-6 1.2-fold. Ultrastructural evaluation of the tissue suggested no direct interaction between the LAB and E. coli, although pathological effects of E. coli in endometrial cells were greatly diminished or even reversed by the LAB combination. This study shows the promising potential of LAB probiotics for therapeutic use against endometrial inflammation and infection.

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“…The probiotic culture used in this study was a mixture of 3 LAB strains isolated previously from the vaginal tract of healthy pregnant cows (Wang et al, 2013;Ametaj et al, 2014). Uterine infections were also monitored but showed no interaction with LAB treatment, which means the effect of LAB treatment on the targeted variables is indepen-dent from uterine infections.…”

Section: Discussionmentioning

confidence: 99%

“…Lactic acid bacteria, mainly lactobacilli, have been utilized to treat urogenital infections in humans (Reid and Bruce, 2003). A previous study conducted by our group reported that 6 doses around calving with a cocktail of 3 LAB isolated from the vaginal tract of healthy cows lowered the incidence of purulent vaginal discharge and improved conception rates of multiparous cows (Ametaj et al, 2014). Based on these results, we hypothesized that reducing the number of treatments around calving from 6 times to 2 or 3 treatments might result in the same beneficial effects.…”

Section: Introductionmentioning

confidence: 99%

Intravaginally administered lactic acid bacteria expedited uterine involution and modulated hormonal profiles of transition dairy cows

Deng

Odhiambo

Farooq

et al. 2015

Journal of Dairy Science

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The objective of this investigation was to evaluate whether intravaginal infusion of lactic acid bacteria (LAB) around parturition could expedite involution rate of the uterus and improve reproductive performance of postpartum dairy cows. One hundred pregnant Holstein dairy cows were assigned to 1 of 3 experimental groups: (1) 1 dose of LAB in wk -2 and -1 and 1 dose of carrier in wk 1 relative to the expected day of parturition (TRT1); (2) 1 dose of LAB in wk -2, -1, and 1 (TRT2); and (3) 1 dose of carrier in wk -2, -1, and 1 (CTR). The LAB treatment was a lyophilized mixture of Lactobacillus sakei FUA3089, Pediococcus acidilactici FUA3138, and Pediococcus acidilactici FUA3140 with a cell count of 10(8) to 10(9) cfu/dose. Uterine involution and ovarian activity was evaluated by transrectal ultrasonography weekly from d 7 to 49 postpartum. Blood samples were collected from a subset of cows to quantify prostaglandin (PG) F2α metabolite (PGFM), PGE2, and progesterone. Cows treated with LAB had smaller cross-sectional areas of gravid horn and uterine body on d 14 postpartum. Cows in TRT2 resumed ovarian cyclicity earlier, as indicated by increased concentrations of serum progesterone. Cows in TRT1 had fewer days open than those in the CTR (110 vs. 150 d), whereas cows in TRT2 and CTR did not differ in days open. In addition, both TRT1 and TRT2 increased the concentrations of PGFM at calving week, and cows in TRT2 also had greater concentrations of PGE2 on d 14 and d 21 postpartum relative to CTR. Overall, cows treated intravaginally with LAB had smaller gravid horn and uterine body on d 14 postpartum than those in the CTR group. Treatment with LAB also increased concentrations of serum PGFM (3,533±328pg/mL in TRT1, 4,470±372pg/mL in TRT2, and 2,000±328pg/mL in CTR on d 0, respectively), with the TRT1 group having fewer cows that resumed ovarian cyclicity but fewer days open compared with both TRT2 and CTR groups. More research is warranted to better understand the mechanism(s) by which intravaginal LAB expedited uterine involution and affected hormonal profiles.

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Intravaginal administration of lactic acid bacteria modulated the incidence of purulent vaginal discharges, plasma haptoglobin concentrations, and milk production in dairy cows

Cited by 37 publications

References 17 publications

Intravaginal probiotics modulated metabolic status and improved milk production and composition of transition dairy cows1

Intravaginal probiotics modulated metabolic status and improved milk production and composition of transition dairy cows1

A combination of lactic acid bacteria regulates Escherichia coli infection and inflammation of the bovine endometrium

Intravaginally administered lactic acid bacteria expedited uterine involution and modulated hormonal profiles of transition dairy cows

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