Intravenous anesthetic propofol suppresses prostaglandin E₂and cysteinyl leukotriene production and reduces edema formation in arachidonic acid-induced ear inflammation

Abstract

Propofol is an intravenous drug widely used for anesthesia and sedation. Previously, propofol was shown to inhibit cyclo-oxygenase (COX) and 5-lipoxygenase (5-LOX) activities. Because these enzyme-inhibiting effects have only been demonstrated in vitro, this study sought to ascertain whether similar effects might also be observed in vivo. In the current studies, effects of propofol were tested in a murine model of arachidonic acid-induced ear inflammation. Specifically, propofol -as a pre-treatment -was intraperitoneally and then topical application of arachidonic acid was performed. After 1 h, tissue biopsies were collected and tested for the presence of edema and for levels of inflammatory mediators. The results indicated that the administration of propofol significantly suppressed ear edema formation, tissue myeloperoxidase activity, and tissue production of both prostaglandin E 2 and cysteinyl leukotrienes. From the data, it can be concluded that propofol could exert anti-COX and anti-5-LOX activities in an in vivo model and that these activities in turn could have, at least in part, suppressed arachidonic acid-induced edema formation in the ear.

Intravenous anesthetic propofol binds to 5‐lipoxygenase and attenuates leukotriene B4production

“…We demonstrated that lesser amounts of LTB 4 , 5‐HETE, 5‐HpETE, 5S,6S‐DiHETE, 5S,6R‐DiHETE, and all‐trans‐LTB 4 were produced in the presence of propofol. The effect of propofol on LTB 4 was reported by Inada et al (36–38), and our result is compatible with theirs. 5S,6S‐DiHETE, 5S,6R‐DiHETE, and all‐trans‐LTB 4 are products of LTA 4 , which is produced via 5‐LOX.…”

Cognitive recovery after ambulatory anaesthesia based on desflurane or propofol: a prospective randomised study

“…Propofol is known to have brochodilatating properties 36,37 , be anticonvulsive 38 and decrease intracerebral bloodflow and intracranial pressure 39,40 . Propofol possesses anti-inflammatory activity 41 and have shown antioxidative effects 42,43 . It also has antiemetic properties 6 17 .…”

Effects of Propofol versus Sevoflurane on Postoperative Pain and Neuroendocrine Stress Response in Oocyte Pickup Patients

“…A study on mice showed that sevoflurane promotes PG E2 production in peritoneal macrophages [13]. Other studies have reported that propofol improves ear edema formation and reduces PGE2 production [14], inhibiting cyclooxygenase activity, and suppressing PG E2 production from dendritic cells [15]. Considering PG E2 regulates fertilization and protects the sperm against phagocytosis in the ovary [16], propofol came to the forefront in our study about PGE2.…”