Intravenous Umbilical Cord Mesenchymal Stem Cell Infusion for the Treatment of Combined Malnutrition Nonunion of the Humerus and Radial Nerve Injury

Abstract

Nonunion and nerve injury are the most severe and common complications of bone fracture treatments. There is still no ideal therapy for these two complications. In this report, we first applied umbilical cord mesenchymal stem cell (UC-MSC) therapy to one patient with both nonunion and nerve injury, and observed the therapeutic effects. UC-MSCs were produced and expanded according to a clinical-grade technique using serum-free medium enriched in human platelet lysate. Flow cytometry was performed to evaluate the purity of UC-MSCs, which were then intravenously injected. At 60 days postinjection, clinical examinations were performed to evaluate the therapeutic effects. Compared with before treatment, the patient's nerve reflex was present, and their muscle tone and strength increased, and x-ray and electromyography analysis further showed that the fracture gap disappeared and the nerve conduction velocity increased with shorter latency and higher amplitude. Furthermore, the clinical evolution was favorable and no side effects were observed during the 1-year follow-up. Overall, this novel treatment might open up a new strategy for the treatment of bone fracture complications.

Trophic Effects of Mesenchymal Stem Cells in Chondrocyte Co-Cultures are Independent of Culture Conditions and Cell Sources

“…17,18 The MSCs isolated from other sources share many common features with BM-MSCs, 19,20 and have been applied in tissueengineering strategies. [21][22][23][24][25] Nevertheless, only a few papers documented the paracrine or autocrine effects of adipose tissue-derived MSCs in tissue regeneration. 26,27 The trophic effects of MSCs isolated from a nonbone marrow origin have not yet been systematically and thoroughly studied.…”

Stem Cell Banking for Regenerative and Personalized Medicine

“…It is only recently that CT stem cells have made their way into the clinic; and it is only now that clinical comparisons to bone marrow MSCs can be made. A recent report from Xue et al examined the use of CT-MSCs in patients with non-healing bone fractures [71]. This study reported significant clinical benefit from intravenously infused MSC.…”

“…In fact, when CB stem cells were placed into animals with fractured femurs there was significant bone healing. A recent report from Xue et al examined the use of CT MSCs in patients with non-healing bone fractures [71]. This study reported significant clinical benefit from intravenously infused MSC.…”

Stem Cell Banking for Regenerative and Personalized Medicine

“…The cryo-container is generally mixed at 4 °C for 20–30 min to allow for cryoprotectant equilibration. Cryopreservation is performed using a controlled rate freezer to −180 °C before final submersion in liquid nitrogen for long term storage [71]. In our experience we have not seen significant differences in AT-MSC harvest by tumescent liposuction, VASER liposuction, power-assisted liposuction or various forms of laser liposuction (although we have not analyzed all possible permutations that are commercially available).…”

“…A recent report from Xue et al . examined the use of CT-MSCs in patients with non-healing bone fractures [71]. This study reported significant clinical benefit from intravenously infused MSC.…”