Intravital Multiphoton Endoscopy

Huland, David M.; Ouzounov, Dimitre G.; Rivera, David R.; Brown, C. M.; Xu, Chris

doi:10.1007/978-94-017-9361-2_15

Cited by 3 publications

(3 citation statements)

References 91 publications

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“…; Huland et al. ), such as the dermoscope and gastrointestinal endoscope. The development of such an instrument is promising for the translation of the proposed method to future clinical use, since our vessel‐type identification strategy does not require any modification of the TPM system itself.…”

Section: Resultsmentioning

confidence: 99%

“…Despite all these restrictions, the proposed vessel-type identification strategy still has an extensive application in anatomic research as a tool for delineating arterial and venous networks and for basic research on the mechanism, diagnosis, and treatment of blood vessel-related diseases. Furthermore, we note that although the TPM system is expensive and complex, there is increasing interest in developing clinical TPM instruments (Konig et al 2007;Huland et al 2014), such as the dermoscope and gastrointestinal endoscope. The development of such an instrument is promising for the translation of the proposed method to future clinical use, since our vessel-type identification strategy does not require any modification of the TPM system itself.…”

Section: Resultsmentioning

confidence: 99%

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In vivo identification of arteries and veins using two‐photon excitation elastin autofluorescence

Yan

et al. 2019

Journal of Anatomy

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Distinguishing arteries from veins in vivo has a great significance in clinical practices and preclinical studies. Optical imaging methods such as two‐photon microscopy can provide high‐resolution morphological information of tissue and are therefore extremely suitable for imaging small blood vessels. However, few optical imaging methods allow in vivo identification of arteries and veins merely utilizing the autofluorescence signal of blood vessels. In this report, we found the arterial wall generates a remarkably stronger two‐photon excitation autofluorescence (TPEA) signal compared with the venous wall based on BALB/c mice. According to histological analysis and fluorescence characteristic measurement, the contrast signal is confirmed to be from elastin fibers. Employing this unique feature, we propose an objective and effective artery–vein separation strategy that considers the presence of the elastin‐TPEA border as the indicator of arteries. Using this strategy, the arterial and venous networks of the dorsal skin and cerebral cortex of BALB/c mice are demonstrated to be excellently mapped and accurately separated in vivo without depending on any exogenous contrast agent, empirical knowledge, and algorithm. This study may provide a novel technique for mapping arterial and venous networks for anatomic research as well as an extra aid to basic researches on the mechanism, diagnosis, and treatment of blood vessel‐related diseases.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

In vivo identification of arteries and veins using two‐photon excitation elastin autofluorescence

Yan

et al. 2019

Journal of Anatomy

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“…This study fills the knowledge gap of human gastric diseases under spectrum-and time-resolved MPM imaging and may shed new light on the early diagnosis of premalignant and malignant lesions of the stomach. We note that there are increasing interests in developing multiphoton endoscopes for clinical use [7,31,32], and these advances make it promising to miniaturize the spectrumand time-resolved MPM for noninvasive, label-free, real-time histological and functional diagnosis of gastric diseases in the future.…”

Section: Introductionmentioning

confidence: 99%

Spectrum- and time-resolved endogenous multiphoton signals reveal quantitative differentiation of premalignant and malignant gastric mucosa

Li¹,

Li²,

He³

et al. 2018

Biomed. Opt. Express

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Early identification of premalignant and malignant gastric mucosa is crucial to decrease the incidence and mortality of stomach cancer. Spectrum- and time-resolved multiphoton microscopy are capable of providing not only structural but also biochemical information at the subcellular level. Based on this multidimensional imaging technique, we performed a systematic investigation on fresh human tissue specimens at the typical stages of gastric carcinogenesis, including normal, chronic gastritis with erosion, chronic gastritis with intestinal metaplasia, and intestinal-type adenocarcinoma. The results demonstrate that this technique is available to characterize the three-dimensional subcellular morphological and biochemical properties of gastric mucosa and further provide quantitative indicators of different gastric disorders, by using endogenous contrast. With advances in multiphoton endoscopy, it has the potential to allow noninvasive, label-free, real-time histological and functional diagnosis of premalignant and malignant lesions of stomach in the future.

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Endoscopic MPM objective designed for depth scanning

Brandt

Liu

et al. 2022

Endoscopic Microscopy XVII

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Intravital Multiphoton Endoscopy

Cited by 3 publications

References 91 publications

In vivo identification of arteries and veins using two‐photon excitation elastin autofluorescence

In vivo identification of arteries and veins using two‐photon excitation elastin autofluorescence

Spectrum- and time-resolved endogenous multiphoton signals reveal quantitative differentiation of premalignant and malignant gastric mucosa

Endoscopic MPM objective designed for depth scanning

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