Intrinsic Elasticity of a Three-Dimensional Macroporous Scaffold Governs the Kinetics of In Situ Biomimetic Reactions

Abstract

Porous materials that synergistically combine high reversible mechanical elasticity with tunable in situ reaction kinetics can find several biological and industrial applications. However, such materials remain elusive in the literature. Herein, we show that by utilizing the intrinsic elastic property of a 3D macroporous material/scaffold comprising polymer-coated biomimetic ceria nanoparticles, the rate of in situ dephosphorylation reactions can be enhanced efficiently. The elasticity of the scaffold is dynamically controlled by employing compression−decompression, [C−D], cycles.Varying the [C−D] frequency from 0 to 4 increases the formation of dephosphorylation reaction products, such as molecular p-nitrophenol or the in situ self-assembled Fmoc-L-tyrosine-based network. Further, we employ a numerical method improvised on an existing stochastic reaction diffusion approach to explain the unusual increase in product formation as a function of the frequency of the [C−D] cycles. The proposed computational methodology predicts rational design of the enzyme-mimicking material by analyzing the efficiency of the product formation under various absolute amounts of the catalyst and substrate levels, suggesting possible applications of such materials. Finally, as a proof of concept, we demonstrate the use of these materials for culturing mammalian cells, which suggests their potential biological applications after implementing appropriate postsynthetic chemical modifications.

Pumping with Modified Polyurethane Sponge: An Alternative Technology for Removing Oil Spills from Water Surface

“…The modified sponge has specific surface area and pore size of 14.48 m 2 g −1 and 140.8 nm, respectively. These large specific surface area and pore size indicate that C‐PDMS‐PU has a honeycomb macroporous structure, which provides the necessary conditions for the storage of oil [26,27] . The same finding can be observed from the SEM image.…”

“…These large specific surface area and pore size indicate that C-PDMS-PU has a honeycomb macroporous structure, which provides the necessary conditions for the storage of oil. [26,27] The same finding can be observed from the SEM image.…”

Understanding the dynamics of complexin-situenzyme-catalyzed reactions undergoing mechanical stress

“…(14)(15); (i) 𝑇 $ , both the product reproduced in almost comparable amounts, (ii) 𝑇 " , the formation rate of product 𝑋 is significantly higher than the product 𝑌 and (iii) 𝑇 . , production of 𝑌 is higher than the creation of 𝑋. I assume that all probable reaction fluxes during [C-D] cycle implementation may get altered under different circumstances.…”

“…13,[15][16][17][18] The synthesized macroporous hybrid biomaterials are extremely efficient for 2D cell culture and tissue development, hence can be introduced in tissue/organs for treating various diseases. 14,[19][20][21] Some of these nano-particle-based materials exhibit conducting properties and they have been used extensively as supporting electrical devices for different organs of the body (heart, brain, and skin). [19][20][21][22][23][24][25][26][27][28] Complex enzyme-based networks that may exhibit competitive substrate binding or multiple intermediates, and cascade reactions are often found in natural biological processes.…”

Metal-Free Carbon Nanozyme as Nicotinamide Adenine Dinucleotide Oxidase Mimic over a Broad pH Range for Coenzyme Regeneration

“…Nanozymes have captured intense attention due to the advantages easy preparation, high stability, and performance diversity, − which have been increasingly applied in the fields of biosensing, , antibacterial application, , disease diagnosis, etc. Up to now, lots of nanomaterials have exhibited oxidase-like activity using universal substrates, such as 3,3′,5,5′-tetramethylbenzidine (TMB) and 2,2′-azino-bis­(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) .…”