Intrinsic hepatic clearance of indocyanine green in the pig: Dependence on plasma protein concentration

Ott, Peter; Keiding, Susanne; Bass, L.

doi:10.1111/j.1365-2362.1992.tb01473.x

Cited by 18 publications

(5 citation statements)

References 29 publications

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“…Although ICG clearance is widely used to estimate liver blood flow, the technique may involve significant assumptions, 33 including that of instantaneous distribution into the plasma compartment, changes in plasma protein concentration that may unpredictably alter intrinsic hepatic clearance of ICG, 34 incomplete extraction, 35 and the accuracy of the assay. 36 However, ICG administration to estimate hepatic blood flow has been considered an especially useful approach in comparative studies.…”

Section: Limitations Of the Studymentioning

confidence: 99%

Modulation by Dietary Salt of Verapamil Disposition in Humans

Darbar

Fromm²,

Dell’Orto³

et al. 1998

Circulation

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Background-The intestine is an increasingly well-recognized site of first-pass drug metabolism. In this study, we determined the influence of dietary salt on the steady-state disposition of verapamil, a drug that undergoes extensive first-pass metabolism. Methods and Results-Eight normal volunteers received 120 mg of racemic verapamil orally twice a day for 21 days. The disposition kinetics of verapamil enantiomers were determined after coadministration of intravenous deuterated verapamil with the morning oral dose on days 7, 14, and 21. Each study day was preceded by 7 days on a fixed-salt diet: in 5 subjects, the initial study was conducted during a low-salt (10 mEq/d) diet, the second study during a high-salt (400 mEq/d) diet, and the third during a low-salt diet, whereas in the other 3 subjects, the sequence of diets was reversed.

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Section: Limitations Of the Studymentioning

confidence: 99%

Modulation by Dietary Salt of Verapamil Disposition in Humans

Darbar

Fromm²,

Dell’Orto³

et al. 1998

Circulation

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“…The doses of ICG were selected on the basis of the data from Rikkers and Moody [23]. Blood samples were collected from the left tail vein before and 2, 4, 6, 10, 15, 20 and 30 min after ICG injection according to a previously reported procedure [2, 9, Indocyanine green (ICG), a tricarbocyanine dye, is normally used to assess hepatic function by measuring the plasma retention of this dye after intravenous administration to either animals [4,7,14,20] or human beings [1,7,15]. ICG possesses various advantages as compared to sulfobromophthalein (BSP); distribution in the plasma volume without extravascular distribution, removal exclusively by the liver into bile without biotransformation, lack of enterohepatic circulation [12,15,19] and low incidence of adverse reactions [1].…”

Section: Animalsmentioning

confidence: 99%

Application of Maximal Removal Rate of Indocyanine Green to the Determination of Hepatic Functional Mass in Conscious Rats.

Inage

Furuhama

1997

J. Vet. Med. Sci.

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ABSTRACT. We established a versatile method for the measurement of indocyanine green maximal removal rate (ICG Rmax) to detect hepatic functional mass in conscious rats using a repeated blood sampling procedure. On investigation of the optimal technical conditions, the appropriate intravenous administered doses of ICG were 2.5, 5, 10 or 20 mg/kg, and the best blood collection times for calculating plasma half-life at these doses were immediately before, and 4, 7 and 10 min after ICG injection. The interval among the respective ICG injections was more than 4 hr. In hepatectomized rats, the ICG Rmax value was reduced to about 50% and 20% of sham-operated rats in mean 2/3 and 4/5 liver resections, respectively, suggesting that it would almost extrapolate to hepatic surviving reserves under these experimental conditions. In rats treated subcutaneously with carbon tetrachloride (CCl 4 , 0.1 and 0.25 ml/kg) thrice weekly during a 17-week period (120 days), a decrease in ICG Rmax value did not correlate with increases in serum alanine transaminase (ALT), alkaline phosphatase (ALP) and total bilirubin values throughout the experimental periods. However, the reduced ICG Rmax well correlated with decreases in serum albumin and cholinesterase (CHE) values from day 50. Histological examinations in the liver revealed that nodules of hepatocytes were separated by thick fibrous bands, defining the typical aspect of cirrhosis on day 30 to 90. These results suggest that the measurement of ICG Rmax is a valuable tool for the estimation of hepatic functional integrity in rats. -KEY WORDS: CCl 4 , hepatectomy, hepatic functional mass, ICG Rmax, rat.J. Vet. Med. Sci. 59 (5): [335][336][337][338][339][340] 1997 serum biochemical compositions such as alanine transaminase (ALT), alkaline phosphatase (ALP), total bilirubin, albumin and cholinesterase (CHE) values in rats treated subcutaneously with carbon tetrachloride (CCl 4 ) for long terms. MATERIALS AND METHODS Animals:Male Sprague-Dawley (SD) rats aged 4 weeks were purchased from Japan S. L. C. (Hamamatsu, Japan), housed 2 or 3 per wire-mesh cage in an air-conditioned room and acclimated to the laboratory environment (temperature, 23 ± 2°C; humidity, 55 ± 15%; light cycle, 12 hr/day) for 1 to 3 weeks. They were given a commercial standard laboratory chow (F-2, Funabashi Farm, Japan) and tap water ad libitum. Before the experiments, the animals were fasted for at least 18 hr with free access to water, unless otherwise stated. All rats were treated humanely, and study protocols were in accordance with our institutional guideline for use of laboratory animals.Measurement of ICG Rmax: Six-week-old SD rats (165-195 g) were divided into five groups of 4 animals each to establish appropriate administration doses of ICG for the measurement of ICG Rmax. Five increasing doses (1.25, 2.5, 5, 10 and 20 mg/kg) of ICG were administered intravenously at an interval of 2 to 4 hr from the lowest dose into the right tail vein of the same rat by use of a 2.5-ml syringe attached to a 25-gauge needl...

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“…3 ICG binds to blood plasma proteins immediately after injection and is cleared from the circulation in approximately 20 minutes. 4,5 Recently, Matsuda et al 6 reported observation of ICG-labeled cells in retinal and choroidal vessels after dye clearance from the plasma. Based on their size and number, the ICG-labeled cells were thought to be circulating leukocytes.…”

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confidence: 99%