Selective Uptake of Indocyanine Green by Reticulocytes in Circulation

Wei, Xunbin; Runnels, Judith; Lin, Charles P.

doi:10.1167/iovs.03-0041

Cited by 28 publications

(24 citation statements)

References 30 publications

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“…The samples were obtained at day 2 after infection. Then these samples were stained with CD71/phycoerythrin and YOYO-1 because CD71 is expressed by most reticulocytes in peripheral blood of rodents (27,28). In noninfected controls, CD71 was almost restricted to the high autofluorescent erythrocytes (Fig.…”

Section: Figmentioning

confidence: 99%

Improvement of detection specificity of Plasmodium‐infected murine erythrocytes by flow cytometry using autofluorescence and YOYO‐1

et al. 2005

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Background: Microscopic analysis of blood smears is currently the most frequently used method to measure parasitemias in experiments of drug efficacy in murine models of malaria. However, it is subjective and labour intensive, which preclude its utilization in large-scale evaluation programs. Flow cytometry is an alternative method, but due to the limited specificity achieved with the currently available techniques, it has not been widely used in murine models of malaria during preclinical evaluation. We describe a new flow cytometric method based on the differences of autofluorescence and DNA content measured after staining with YOYO-1 that are observed in infected erythrocytes compared with noninfected erythrocytes. Methods: Samples of blood from Plasmodium yoeliiinfected animals were fixed with glutaraldehyde, incubated with RNAase, and stained with YOYO-1 in 96-well plate format. After acquisition, erythrocytes gated in loga-

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Section: Figmentioning

confidence: 99%

Improvement of detection specificity of Plasmodium‐infected murine erythrocytes by flow cytometry using autofluorescence and YOYO‐1

et al. 2005

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“…Indocianine green (ICG) is a well-known, relatively harmless dye for IR blood angiography and IR imaging of lymph nodes in human and animals [196][197][198][199][200][201] . Within the first seconds after intravenous injection, there is a shift in the absorption spectrum of ICG from 780 nm to 805 nm (spectral stabilization) due to two processes: polymerization of ICG molecules and their binding to plasma proteins and lipoproteins [196] .…”

Section: Ir Angiography and Lymphgraphymentioning

confidence: 99%

Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening

Galanzha¹

2007

WJG

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Using animal mesentery with intravital optical microscopy is a well-established experimental model for studying blood and lymph microcirculation in vivo . Recent advances in cell biology and optical techniques provide the basis for extending this model for new applications, which should generate significantly improved experimental data. This review summarizes the achievements in this specific area, including in vivo label-free blood and lymph photothermal flow cytometry, super-sensitive fluorescence image cytometry, light scattering and speckle flow cytometry, microvessel dynamic microscopy, infrared (IR) angiography, and high-speed imaging of individual cells in fast flow. The capabilities of these techniques, using the rat mesentery model, were demonstrated in various studies; e.g., real-time quantitative detection of circulating and migrating individual blood and cancer cells, studies on vascular dynamics with a focus on lymphatics under normal conditions and under different interventions (e.g. lasers, drugs, nicotine), assessment of lymphatic disturbances from experimental lymphedema, monitoring cell traffic between blood and lymph systems, and high-speed imaging of cell transient deformability in flow. In particular, the obtained results demonstrated that individual cell transportation in living organisms depends on cell type (e.g., normal blood or leukemic cells), the cell's functional state (e.g., live, apoptotic, or necrotic), and the functional status of the organism. Possible future applications, including in vivo early diagnosis and prevention of disease, monitoring immune response and apoptosis, chemo-and radio-sensitivity tests, and drug screening, are also discussed. TOPIC HIGHLIGHT

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“…This in vivo approach allows potentially assessing rare CTCs in much larger blood volume compared to sample volume ex vivo . The most frequently used fluorescent labeling of cells in bloodstream demonstrated promising results on animal models although translation of this approach to human may be problematic due to (1) potential toxicity of the available fluorescent labels; (2) immune response to fluorescent tags; and (3) influence of light scattering and autofluorescence of the background, which limit its application to the assessment of superficial microvessels with a slow flow rate that may significantly lengthen the examination of the large blood volume ([13,14] and references there). …”

Section: Introductionmentioning

confidence: 99%

Nanotechnology‐based molecular photoacoustic and photothermal flow cytometry platform for in‐vivo detection and killing of circulating cancer stem cells

Galanzha¹,

Kim

Zharov

2009

Journal of Biophotonics

120

130

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In‐vivo multicolor photoacoustic (PA) flow cytometry for ultrasensitive molecular detection of the CD44+ circulating tumor cells (CTCs) is demonstrated on a mouse model of human breast cancer. Targeting of CTCs with stem‐like phenotype, which are naturally shed from parent tumors, was performed with functionalized gold and magnetic nanoparticles. Results in vivo were verified in vitro with a multifunctional microscope, which integrates PA, photothermal (PT), fluorescent and transmission modules. Magnet‐induced clustering of magnetic nanoparticles in individual cells significantly amplified PT and PA signals. The novel noninvasive platform, which integrates multispectral PA detection and PT therapy with a potential for multiplex targeting of many cancer biomarkers using multicolor nanoparticles, may prospectively solve grand challenges in cancer research for diagnosis and purging of undetectable yet tumor‐initiating cells in circulation before they form metastasis. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)

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Selective Uptake of Indocyanine Green by Reticulocytes in Circulation

Abstract: ICG strongly labels reticulocytes and weakly labels neutrophils. To the authors' knowledge, this is the first report of selective staining of reticulocytes by ICG.

Cited by 28 publications

References 30 publications

Improvement of detection specificity of Plasmodium‐infected murine erythrocytes by flow cytometry using autofluorescence and YOYO‐1

Improvement of detection specificity of Plasmodium‐infected murine erythrocytes by flow cytometry using autofluorescence and YOYO‐1

Advances in small animal mesentery models for in vivo flow cytometry, dynamic microscopy, and drug screening

Nanotechnology‐based molecular photoacoustic and photothermal flow cytometry platform for in‐vivo detection and killing of circulating cancer stem cells

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