Intrinsic peroxidase-like activity of mesoporous nickel oxide for selective cysteine sensing

Ray, Chaiti; Dutta, Soumen; Sarkar, Sougata; Sahoo, Ramkrishna; Roy, Anindita; Pal, Tarasankar

doi:10.1039/c4tb00968a

Cited by 108 publications

(58 citation statements)

References 49 publications

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“…[1][2][3][4][5][6] Enzymes are typically proteins that catalyze bio-chemical reactions in the cellular metabolic processes and they enhance rate of the reactions. 7,8 Horseradish peroxidase (HRP) is a natural peroxidase enzyme which is extracted from plants and it catalyzes decomposition of peroxides and oxidation of various substrates.…”

Section: Introductionmentioning

confidence: 99%

A facile synthetic approach for SiO₂@Co₃O₄ core–shell nanorattles with enhanced peroxidase-like activity

Kandula

Jeevanandam

2015

RSC Adv.

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SiO2@Co3O4 coreshell nanorattles with different Co3O4 shell thickness have been successfully synthesized by the calcination of SiO2@-Co(OH)2 at 500 o C. The synthetic approach is facile, economical, and requires no surface modification. The synthesized materials were thoroughly characterized using powder Xray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), thermal gravimetric analysis (TGA), BrunauerEmmettTeller (BET) analysis, field emission scanning electron microscopy (FESEM), transmission electron microscopy (TEM), and diffuse reflectance spectroscopy (DRS). SEM analysis indicates hierarchical coreshell morphology for SiO2@Co3O4 and the TEM results indicate the coreshell nanorattle morphology. Diffuse reflectance spectroscopy studies indicate that the SiO2@Co3O4 coreshell nanorattles show two absorption bands in the range 420450 nm and 700750 nm related to ligand to metal charge transfer transitions (O 2 → Co 2+ and O 2 → Co 3+ ). The SiO2@Co3O4 coreshell nanorattles act as an artificial peroxidase enzyme mimic with enhanced intrinsic peroxidaselike activity compared to pure Co3O4 nanoparticles and horseradish peroxidase (HRP), a natural enzyme. The SiO2@Co3O4 coreshell nanorattles show higher kcat and kcat/Km values compared to pure Co3O4 and HRP indicating their applicability as artificial enzyme mimic in biomedicine and biosensing.

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Section: Introductionmentioning

confidence: 99%

A facile synthetic approach for SiO₂@Co₃O₄ core–shell nanorattles with enhanced peroxidase-like activity

Kandula

Jeevanandam

2015

RSC Adv.

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“…25,26 This colorimetric assay further executed as a simple, low cost and highly effective platform for biosensing application. 25 Over the years this protocol has been adopted for colorimetric determination of various molecules and ions such as glucose, 25 nucleotides, 27 H 2 O 2 , 8 ascorbic acid, 23 amino acids, 8 melamine, 28 and Hg 2+ , 29 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 Graphene, a two-dimensional (2D) sp 2 hybridized thin atomic layer of carbon has become an interesting area of research due to its various intriguing properties such as high thermal and electrical conductivity, outstanding mechanical strength and remarkable surface area etc. 31, 32 Graphene oxide (GO) is generally used as a starting material for the wet chemical synthesis of graphene and its numerous derivatives.…”

Section: Introductionmentioning

confidence: 99%

A Gel-Based Approach To Design Hierarchical CuS Decorated Reduced Graphene Oxide Nanosheets for Enhanced Peroxidase-like Activity Leading to Colorimetric Detection of Dopamine

et al. 2015

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Supramolecular colorless copper(I)-thiourea hydrogel (Cu-TU gel) has been made a mechanically strong functional hybrid material in graphene oxide (GO) framework.Mild heat treatment (85 ºC) of the hybrid material fetches black colored hierarchical copper sulfide decorated reduced graphene oxide nanosheets (CuS-rGO) through an obvious inhouse redox transformation reaction between Cu(I) and GO without any additive. The assynthesized CuS-rGO nanocomposite exhibits impressive peroxidase-like activity where oxidation of colorless 3, 3', 5, 5'-tetramethylbenzidine (TMB) to blue colored product is observed in solution phase with H 2 O 2 . Systematic control experiments suggest that strong covalent interaction between CuS and rGO synergistically enhances the catalytic activity of CuS-rGO in comparison to its individual counterparts. Furthermore, important biomolecule, dopamine has been found to selectively inhibit, in succession, the oxidizing action of H 2 O 2 for TMB oxidation reaction. Thus dopamine dependent successive inhibition reaction creates a one-pot reporter platform to sense dopamine down to 0.48 µM concentration level by UVvis spectrophotometry.

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“…2 It is also a potential neurotoxin, 3,4 a biomarker for various medical examinations, 5,6 and it causes several diseases. Most of the sensing techniques follow a colorimetric pathway, 11 electrochemical methodology, 12 UV-vis spectroscopy, 13 mass spectrometry, 14 resonance light scattering (RLS) technique, 15 and circular dichroism technique. Cys deficiency is involved in slow growth, hair depigmentation, edema, lethargy, liver damage, muscle and fat loss, skin lesions, and weakness.…”

Section: Introductionmentioning

confidence: 99%

Intriguing cysteine induced improvement of the emissive property of carbon dots with sensing applications

Jana

Ganguly

Pal

2015

Phys. Chem. Chem. Phys.

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A simple fluorometric technique has been adopted for cysteine (Cys) sensing in alkaline medium down to the nM level. The huge fluorescent signal of the solution is a consequence of fluorescent carbon dots (CDs) produced in situ from modified hydrothermal (MHT) reaction between Cys and dopamine (DA). It has been observed that the inherent fluorescence of DA is drastically quenched in alkaline solution. Cys can selectively rescue the fluorescence of DA. Thus, Cys determination in a straightforward way, but only to a micro molar (10(-7) M i.e. 0.1 μM) level is possible through such fluorescence enhancement. Sensitive Cys determination remains associated with the in situ generated CDs, but the external addition of pre-formed CDs to Cys solution fails miserably towards Cys detection. However, CDs prepared from the Cys-DA system in alkaline solution admirably increase the limit of detection (LOD) of Cys at least two orders higher (10(-9) M) than that observed without hydrothermal technique i.e., without CDs. This method finds applications for Cys determination in biological samples and pharmaceutical preparations.

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Intrinsic peroxidase-like activity of mesoporous nickel oxide for selective cysteine sensing

Cited by 108 publications

References 49 publications

A facile synthetic approach for SiO₂@Co₃O₄ core–shell nanorattles with enhanced peroxidase-like activity

A facile synthetic approach for SiO₂@Co₃O₄ core–shell nanorattles with enhanced peroxidase-like activity

A Gel-Based Approach To Design Hierarchical CuS Decorated Reduced Graphene Oxide Nanosheets for Enhanced Peroxidase-like Activity Leading to Colorimetric Detection of Dopamine

Intriguing cysteine induced improvement of the emissive property of carbon dots with sensing applications

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Intrinsic peroxidase-like activity of mesoporous nickel oxide for selective cysteine sensing

Cited by 108 publications

References 49 publications

A facile synthetic approach for SiO2@Co3O4 core–shell nanorattles with enhanced peroxidase-like activity

A facile synthetic approach for SiO2@Co3O4 core–shell nanorattles with enhanced peroxidase-like activity

A Gel-Based Approach To Design Hierarchical CuS Decorated Reduced Graphene Oxide Nanosheets for Enhanced Peroxidase-like Activity Leading to Colorimetric Detection of Dopamine

Intriguing cysteine induced improvement of the emissive property of carbon dots with sensing applications

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A facile synthetic approach for SiO₂@Co₃O₄ core–shell nanorattles with enhanced peroxidase-like activity

A facile synthetic approach for SiO₂@Co₃O₄ core–shell nanorattles with enhanced peroxidase-like activity