Introducing a new Bioengineered Bug:<i>Methylobacterium extorquens</i>tuned as a microbial bioplastic factory

Höfer, Philipp; Vermette, Patrick; Groleau, Denis

doi:10.4161/bbug.2.2.15009

Cited by 14 publications

(10 citation statements)

References 29 publications

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“…Examples of such biopolymers are the biodegradable polyesters polyhydroxybutyric acid (PHB) and polyhydroxyalkanoate (PHA). M. extorquens was genetically modified to increase PHB and PHA production using methanol as a substrate [ 116 ]. M. organophilum was also reported to accumulate PHB and PHA [ 117 ] under nitrogen limitation while biodegrading methane (a greenhouse gas).…”

Section: Biotechnological Uses Of Methylobacterium mentioning

confidence: 99%

Biotechnological and Agronomic Potential of Endophytic Pink-Pigmented MethylotrophicMethylobacteriumspp.

Dourado

Neves

Santos

et al. 2015

BioMed Research International

133

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The genus Methylobacterium is composed of pink-pigmented facultative methylotrophic (PPFM) bacteria, which are able to synthesize carotenoids and grow on reduced organic compounds containing one carbon (C1), such as methanol and methylamine. Due to their high phenotypic plasticity, these bacteria are able to colonize different habitats, such as soil, water, and sediment, and different host plants as both endophytes and epiphytes. In plant colonization, the frequency and distribution may be influenced by plant genotype or by interactions with other associated microorganisms, which may result in increasing plant fitness. In this review, different aspects of interactions with the host plant are discussed, including their capacity to fix nitrogen, nodule the host plant, produce cytokinins, auxin and enzymes involved in the induction of systemic resistance, such as pectinase and cellulase, and therefore plant growth promotion. In addition, bacteria belonging to this group can be used to reduce environmental contamination because they are able to degrade toxic compounds, tolerate high heavy metal concentrations, and increase plant tolerance to these compounds. Moreover, genome sequencing and omics approaches have revealed genes related to plant-bacteria interactions that may be important for developing strains able to promote plant growth and protection against phytopathogens.

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Section: Biotechnological Uses Of Methylobacterium mentioning

confidence: 99%

Biotechnological and Agronomic Potential of Endophytic Pink-Pigmented MethylotrophicMethylobacteriumspp.

Dourado

Neves

Santos

et al. 2015

BioMed Research International

133

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“…Insulin (Walsh, 2005); vanillin (Hansen et al, 2009; Brochado et al, 2010); antibodies such as penicillin (Kiel et al, 2005); and industrially valuable enzymes such as lipase (Pandey et al, 1999), cellulase (Kuhad et al, 2011), and amylase (Pandey et al, 2000) are already being produced in large scale with microbial factories. Apart from enzyme production, microbes are also being engineered to produce industrially important nanoparticles that are used in electronics and drug delivery (Dhanjal and Cameotra, 2010; Villaverde, 2010), environmentally beneficial bioplastics (Höfer et al, 2011), insect silk (Scheibel, 2004), opiates (Thodey et al, 2014), biofuels such as isobutanol (Desai et al, 2015), various chemicals (Vázquez et al, 2010), and proteins (Ferrer-Miralles et al, 2009). These industrial bioreactors are relatively inexpensive and efficient.…”

Section: Introductionmentioning

confidence: 99%

Exploiting Self-organization in Bioengineered Systems: A Computational Approach

Davis

Doloman

Podgorski

et al. 2017

Front. Bioeng. Biotechnol.

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The productivity of bioengineered cell factories is limited by inefficiencies in nutrient delivery and waste and product removal. Current solution approaches explore changes in the physical configurations of the bioreactors. This work investigates the possibilities of exploiting self-organizing vascular networks to support producer cells within the factory. A computational model simulates de novo vascular development of endothelial-like cells and the resultant network functioning to deliver nutrients and extract product and waste from the cell culture. Microbial factories with vascular networks are evaluated for their scalability, robustness, and productivity compared to the cell factories without a vascular network. Initial studies demonstrate that at least an order of magnitude increase in production is possible, the system can be scaled up, and the self-organization of an efficient vascular network is robust. The work suggests that bioengineered multicellularity may offer efficiency improvements difficult to achieve with physical engineering approaches.

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“…Potassium-limitation of Mtb organophilum had also been shown previously to enhance co-polyalkanoate formation (Kim et al 1999). Using both feeding with different n-alkenoic acids and genetic modification of a proprietary Methylobacterium strain could enable production of the ''functionalized'' polyhydroxyalkanoates required as advanced materials for tissue engineering, biocomposites, biodegradable polymers, and several medical uses (Höfer et al 2010(Höfer et al , 2011.…”

Section: Methylobacterium In Biotechnology and Biodeteriorationmentioning

confidence: 99%

“…Methylobacterium strains have been applied commercially to fermentation processes in the manufacture of coenzyme Q-10, lysine, tyrosine, phenylalanine, vitamin B12, enzymes, bioactive peptides, bio-insecticides, biopolymers such as polyb-hydroxybutyrate (PHB), and copolymeric polyalkanoates (Bormann et al 1997;Bourque et al 1995;Desmarteaux et al 2012;Green 2006;Höfer et al 2011;Kim et al 1999;Yezza et al 2006). Methylobacterium strains were never regarded as likely candidates for the commercial production of single-cell-protein as a foodstock, or for biosynthesis of deuterated and 13 C/ 15 Nlabeled ribonucleotides, as their growth yields (protein produced/mol methanol consumed) were inferior to those of methylotrophs such as Methylophilus methylotrophus, which used the Quayle cycle for C 1 -assimilation, rather than the serine pathway found in Methylobacterium (Batey et al 1996;Schrader et al 2009;Westlake 1986).…”

Section: Methylobacterium In Biotechnology and Biodeteriorationmentioning

confidence: 99%