Introducing polymer conductance in diagnostically relevant transduction

Baradokė, Aušra; Santos, Alexandre Leme Godoy dos; Bueno, Paulo Agenor Alves; Davis, Jason J.

doi:10.1016/j.bios.2020.112705

Cited by 6 publications

(5 citation statements)

References 30 publications

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“…Traditional R ct EIS, which uses the charge transfer between an electrode and diffusing redox probes in solution, cannot show the quantum resonant conductance because the measurement of quantum resonant conductance needs DOS exchanging, generated from charge/discharge of the redox probe anchored to an electrode surface, not from redox probes in solution. , As described in Section , quantum EIS using the immobilized PMB redox probes on the GCE surface was performed to investigate quantum electrochemical properties at the interface of nanoscale MICP films, such as electrochemical capacitance ( C μ ), RC time constant terms (τ), and PMB charging resistance ( R q ). The impedimetric Nyquist plot shows the decrement of the imaginary impedance component ( Z im ), providing a measure of the capacitance part of the impedance upon the binding of target cortisol to the receptors of the MICP film (Figure A).…”

Section: Resultsmentioning

confidence: 99%

Introducing Nanoscale Electrochemistry in Small-Molecule Detection for Tackling Existing Limitations of Affinity-Based Label-Free Biosensing Applications

Lee,

Kim

2023

J. Am. Chem. Soc.

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Electrochemical sensing techniques for small molecules have progressed in many applications, including disease diagnosis and prevention as well as monitoring of health conditions. However, affinity-based detection for low-abundance small molecules is still challenging due to the imbalance in target-to-receptor size ratio as well as the lack of a highly sensitive signal transducing method. Herein, we introduced nanoscale electrochemistry in affinity-based small molecule detection by measuring the change of quantum electrochemical properties with a nanoscale artificial receptor upon binding. We prepared a nanoscale molecularly imprinted composite polymer (MICP) for cortisol by electrochemically copolymerizing β-cyclodextrin and redoxactive methylene blue to offer a high target-to-receptor size ratio, thus realizing "bind-and-read" detection of cortisol as a representative target small molecule, along with extremely high sensitivity. Using the quantum conductance measurement, the present MICP-based sensor can detect cortisol from 1.00 × 10 −12 to 1.00 × 10 −6 M with a detection limit of 3.93 × 10 −13 M (S/N = 3), which is much lower than those obtained with other electrochemical methods. Moreover, the present MICP-based cortisol sensor exhibited reversible cortisol sensing capability through a simple electrochemical regeneration process without cumbersome steps of washing and solution change, which enables "continuous detection". In situ detection of cortisol in human saliva following circadian rhythm was carried out with the present MICP-based cortisol sensor, and the results were validated with the LC−MS/MS method. Consequently, this present cortisol sensor based on nanoscale MICP and quantum electrochemistry overcomes the limitations of affinity-based biosensors, opening up new possibilities for sensor applications in point-of-care and wearable healthcare devices.

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Section: Resultsmentioning

confidence: 99%

Introducing Nanoscale Electrochemistry in Small-Molecule Detection for Tackling Existing Limitations of Affinity-Based Label-Free Biosensing Applications

Lee,

Kim

2023

J. Am. Chem. Soc.

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“…Phosphate buffer is the most commonly used buffer for the investigation of electrochemical sensing, where neutral pH is required [1,3,4]. Therefore, CVs in 7.4 pH PB at different scan rates (10,20,50,100,200, and 400 mVs −1 ) were performed.…”

Section: Characterisation Of Cfmb Electrode In Phosphate Buffermentioning

confidence: 99%

“…Recently, carbon-based materials have become the most commonly used electrode materials in electrochemical applications due to their excellent electrical conductivity, low cost, and ease of modification [1][2][3][4][5][6].…”

Section: Introductionmentioning

confidence: 99%

Examination of Non-Modified Carbon Fibre Bundle as an Electrode for Electrochemical Sensing

Elsakova,

Merzlikin,

Jafarov

et al. 2023

Coatings

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This study presents a simple and cost-effective method for producing carbon fibre microcylinder bundle (CFMB) electrodes that are highly stable and reproducible for electrochemical sensing applications. The CFMBs were integrated into a 3D-printed electrochemical cell and tested for dopamine (DA) detection. The results demonstrated a linear increase in current with increasing DA concentration, reaching a sensitivity of 428 nAμM−1 and a limit of detection (LOD) of 8.85 μM. The CFMBs also showed high electrochemical selectivity for DA due to the similar oxidation potentials of dopamine and the chemical groups present on the surface of the CFMBs. The reproducibility of the CFMBs was also demonstrated by the low variation in background currents between different electrodes. These findings highlight the potential of CFMBs as a low-cost and effective platform for electrochemical sensing applications.

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“…Reagentless biological sensors have been designed for point-of-care platforms with minimal intervention for sample analysis. − These devices are primarily categorized based on the fact that there is no need to add a redox probe or any other reagents to scrutinize the target sample. ,,, To date, the development of reagentless sensing interfaces has been primarily based on thiol , and peptide self-assembled monolayers or alternatively on Prussian Blue and redox polymer nanoscale films . The disadvantage of this type of soft matter interface is associated with the low ability to harness oxidative environments; commercialization of these devices requires long-term interfacial durability.…”

Section: Introductionmentioning

confidence: 99%

Density of States of a Nanoscale Semiconductor Interface as a Transduction Signal for Sensing Molecules

Pinzón

Santos

Bueno

2021

ACS Appl. Electron. Mater.

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Nanoscale inorganic semiconductors (with interfacial thickness <10 nm) have superior stability compared to soft organic compounds and are important for the development of enhanced reagentless sensing devices. As a proof of concept, this study demonstrated that the density of states of a copper oxide mixed valence semiconducting nanoscale film can be suitably designed as a transducer signal for reagentless sensing of phenol.

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Introducing polymer conductance in diagnostically relevant transduction

Cited by 6 publications

References 30 publications

Introducing Nanoscale Electrochemistry in Small-Molecule Detection for Tackling Existing Limitations of Affinity-Based Label-Free Biosensing Applications

Introducing Nanoscale Electrochemistry in Small-Molecule Detection for Tackling Existing Limitations of Affinity-Based Label-Free Biosensing Applications

Examination of Non-Modified Carbon Fibre Bundle as an Electrode for Electrochemical Sensing

Density of States of a Nanoscale Semiconductor Interface as a Transduction Signal for Sensing Molecules

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