Introduction

Abstract

A range of molecular methods can be employed for the characterization of natural and induced nucleotide variation in plants. These facilitate a better understanding of gene function and allow a reduction in the time needed to breed new mutant varieties. Molecular biology, however, can be difficult to master, and while efficient, many protocols rely on expensive pre-made kits. The FAO/IAEA Plant Breeding and Genetics Laboratory (PBGL) has developed a series of low-cost and easy to use approaches for the molecular characterization of mutant plant materials. The protocols are designed specifically to avoid complicated procedures, expensive equipment, and the use of hazardous chemicals. Furthermore, these protocols have been validated by research fellows from many developing countries.