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Cited by 5 publications
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The local heating of poly(3,4-ethylenedioxythiophene) (PEDOT) by ap hotothermal effect directed by nearinfrared (NIR) light induces unfolding of absorbed collagen triple helices,yielding soluble collagen single-helical structures. This dissociation of collagens allowed the harvesting of aliving idiomorphic cell sheet, achieved upon irradiation with NIR light (l = 808 nm). The PEDOTlayer was patterned and cells were successfully cultured on the patterned substrate.C ell sheets of various shapes mirroring the PEDOTp attern could be detached after af ew minutes of irradiation with NIR light. The PEDOTp atterns guided not only the entire shape of the cell sheets but also the spreading direction of the cells in the sheets.T his photothermally induced dissociation of collagen provided af ast non-invasive harvesting method and tailormade cell-sheet patterns.Collagen, which has at riple-helical structure,i st he one of the most important proteins in cell science. [1][2][3][4] Each triple helixisstabilized by numerous hydrogen bonds and supports cell binding.H owever,t he triple helix of collagens unfolds upon heating. [5] Therefore,c ontrolling the dissociation of collagen could be highly important for cell-to-surface interactions and eventually for the detachment of cells.Most celldetachment studies are undertaken using temperatureresponsive culture dishes on which thermoresponsive polymers,s uch as poly(N-isopropylacrylamide) (pNIPAAm), are covalently grafted. [6][7][8][9][10] Although this is one of the most promising methods and is well-established, spatial control of dissociation is particularly difficult using these thermoresponsive substrates.N ear-IR (NIR) laser irradiation is "biologically friendly" and can be employed to provide au nique method to spatially control cell behavior. [11][12][13][14] By spatially dissociating collagens through the photothermal effect, we now describe,for the first time,tunable cell-sheet detachment and harvesting from ap oly(3,4-ethylenedioxythiophene) (PEDOT) substrate without achange in the cell morphology.PEDOTwas directly coated onto polystyrene by polymer solution casting to form SP-PEDOT (PEDOT made by solution casting polymerization (SP)). Then collagen type I (0.3 wt %) was dropcast onto the SP-PEDOT substrate.T he thickness of the collagen layer was 14-18 mm, as determined using an Alpha-Step surface profiler.Fibroblasts were seeded on top of ac ollagen-coated PEDOT substrate (CSP-PEDOT) at ac oncentration of 310 5 cells/dish and were cultured for 3days.T he CSP-PEDOTw as irradiated with aN IR diode laser (l = 808 nm), with an input power density (I pw )between 1.9 Wcm À2 and 2.5 Wcm À2 (see Table S1 in the Supporting Information). When exposed to NIR light, the temperature of the CSP-PEDOTw as increased to 41.4 8 8C ( Figure S1). After 5min of irradiation, the cell sheets were detached from the substrate (Figure 1a). Theh arvested cell sheets showed positive E-cadherin expression (Figure 1b), verifying that cell-cell interactions are maintained in the harvested cell sheet...
The local heating of poly(3,4-ethylenedioxythiophene) (PEDOT) by ap hotothermal effect directed by nearinfrared (NIR) light induces unfolding of absorbed collagen triple helices,yielding soluble collagen single-helical structures. This dissociation of collagens allowed the harvesting of aliving idiomorphic cell sheet, achieved upon irradiation with NIR light (l = 808 nm). The PEDOTlayer was patterned and cells were successfully cultured on the patterned substrate.C ell sheets of various shapes mirroring the PEDOTp attern could be detached after af ew minutes of irradiation with NIR light. The PEDOTp atterns guided not only the entire shape of the cell sheets but also the spreading direction of the cells in the sheets.T his photothermally induced dissociation of collagen provided af ast non-invasive harvesting method and tailormade cell-sheet patterns.Collagen, which has at riple-helical structure,i st he one of the most important proteins in cell science. [1][2][3][4] Each triple helixisstabilized by numerous hydrogen bonds and supports cell binding.H owever,t he triple helix of collagens unfolds upon heating. [5] Therefore,c ontrolling the dissociation of collagen could be highly important for cell-to-surface interactions and eventually for the detachment of cells.Most celldetachment studies are undertaken using temperatureresponsive culture dishes on which thermoresponsive polymers,s uch as poly(N-isopropylacrylamide) (pNIPAAm), are covalently grafted. [6][7][8][9][10] Although this is one of the most promising methods and is well-established, spatial control of dissociation is particularly difficult using these thermoresponsive substrates.N ear-IR (NIR) laser irradiation is "biologically friendly" and can be employed to provide au nique method to spatially control cell behavior. [11][12][13][14] By spatially dissociating collagens through the photothermal effect, we now describe,for the first time,tunable cell-sheet detachment and harvesting from ap oly(3,4-ethylenedioxythiophene) (PEDOT) substrate without achange in the cell morphology.PEDOTwas directly coated onto polystyrene by polymer solution casting to form SP-PEDOT (PEDOT made by solution casting polymerization (SP)). Then collagen type I (0.3 wt %) was dropcast onto the SP-PEDOT substrate.T he thickness of the collagen layer was 14-18 mm, as determined using an Alpha-Step surface profiler.Fibroblasts were seeded on top of ac ollagen-coated PEDOT substrate (CSP-PEDOT) at ac oncentration of 310 5 cells/dish and were cultured for 3days.T he CSP-PEDOTw as irradiated with aN IR diode laser (l = 808 nm), with an input power density (I pw )between 1.9 Wcm À2 and 2.5 Wcm À2 (see Table S1 in the Supporting Information). When exposed to NIR light, the temperature of the CSP-PEDOTw as increased to 41.4 8 8C ( Figure S1). After 5min of irradiation, the cell sheets were detached from the substrate (Figure 1a). Theh arvested cell sheets showed positive E-cadherin expression (Figure 1b), verifying that cell-cell interactions are maintained in the harvested cell sheet...
The local heating of poly(3,4-ethylenedioxythiophene) (PEDOT) by a photothermal effect directed by near-infrared (NIR) light induces unfolding of absorbed collagen triple helices, yielding soluble collagen single-helical structures. This dissociation of collagens allowed the harvesting of a living idiomorphic cell sheet, achieved upon irradiation with NIR light (λ=808 nm). The PEDOT layer was patterned and cells were successfully cultured on the patterned substrate. Cell sheets of various shapes mirroring the PEDOT pattern could be detached after a few minutes of irradiation with NIR light. The PEDOT patterns guided not only the entire shape of the cell sheets but also the spreading direction of the cells in the sheets. This photothermally induced dissociation of collagen provided a fast non-invasive harvesting method and tailor-made cell-sheet patterns.
ABSTRACT:The experimental results on the development of thin ($ 1.5 lm) gelatin-based coatings and the investigation on their sealing attribute when applied onto oriented polypropylene (OPP) are reported. The sealing performance, expressed as the strain energy required to separate the sealed joints, was studied as a function of three different influencing factors. pH of the hydrogel solution was varied between 5 and 11. The highest seal strength values were obtained for pH values beyond the isoelectric point (IEP) of the gelatin molecule. The effect of the plasticizer (glycerol) was studied by changing its concentration from 2.5 wt % to 7.5 wt % to the total weight of the hydrogel solution. Glycerol concentration ¼ 7.5 wt % was found to be the best for achieving adequate strain energy values. The influence of a hydrophobic component on the capability of the coating to act as a sealant has also been assessed. The hydrophobic component had a positive effect only up to a certain level (1 wt %, weight percent), whereas beyond this value, it affected the seal strength attribute. According to the best setting conditions, seal strength values for the OPP biocoated films of $ 61 N Â mm were attained, with a corresponding maximum force required to break the joints of 2.4 N. These results are discussed by taking into consideration the modality of seals opening. Interestingly, the heat-seal (temperature: 90 C; dwell time: 1 s; pressure: 4 bar) failed in both peeling and tearing mode failure, as confirmed by microscopy, spectrophotometric, and particle size analyzes.
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