Introduction and expression of the Streptomyces cholesterol oxidase gene (ChoA), a potent insecticidal protein active against boll weevil larvae, into tobacco cells

Cho, H. J.; Choi, Kwang Pil; Yamashita, Mitsuo; Morikawa, Hiromichi; Murooka, Y

doi:10.1007/bf00164492

Cited by 35 publications

(4 citation statements)

References 21 publications

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“…This enzyme is industrially important and widely used for the determination of cholesterol in food and blood serum by coupling with peroxidase [10,11], and also in the production of starting material for the chemical synthesis of pharmaceutical steroids. Cholesterol oxidase also exhibits potent insecticidal activity that is very important and vital for pest control strategies employing transgenic crops [12,13].…”

Section: Introductionmentioning

confidence: 99%

Affinity purification of a cholesterol oxidase expressed in Escherichia coli

Xin

Yang

Xia

et al. 2011

Journal of Chromatography B

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Section: Introductionmentioning

confidence: 99%

Affinity purification of a cholesterol oxidase expressed in Escherichia coli

Xin

Yang

Xia

et al. 2011

Journal of Chromatography B

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“…Bacillus thuringiensis, Vaeck et al, 1987). Transgenic approaches have also been used to introduce other insecticidal genes into plants such as inhibitors of digestive enzymes (Hilder et al, 1987;Huesing et al, 1991), resistance genes from plants (Dowd et al, 1998;Felton et al, 1992), and micro-organisms (Cho et al, 1995;Estruch et al, 1996;Vaeck et al, 1987;Warren et al, 1996), lectin genes (Cavalieri et al, 1995;Chrispeels and Raikhel, 1991), tryptophan decarboxylase (Thomas et al, 1995), and chitinases (Gatehouse et al, 1996) for the purpose of controlling insect herbivores (recently reviewed by Schuler et al, 1998, and references therein). However, parasitoid genes have not, previously, been expressed in transgenic plants for insect control.…”

Section: Introductionmentioning

confidence: 99%

Antibiosis‐type insect resistance in transgenic plants expressing a teratocyte secretory protein (TSP14) gene from a hymenopteran endoparasite (Microplitis croceipes)

Maiti¹,

Dey

Pattanaik³

et al. 2003

Plant Biotechnology Journal

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SummaryWe have isolated a teratocyte secretory protein (TSP14) gene product from a hymenopteran endoparasite that disrupts the growth of lepidopteran insect larvae. To evaluate the insecticidal activity of TSP14 for the protection of crops from insect damage, chimeric gene constructs of TSP14 were expressed in transgenic plants. The coding sequence of the TSP14 gene, with and without its native signal peptide, was placed between the modified peanut chlorotic streak virus (PClSV) full-length transcript (FLt) promoter with duplicated enhancer domains and the terminator sequence from the rbcSE9 gene. These chimeric genes, expressed in transgenic tobacco (Nicotiana tabacum cv. Samsun NN) were stably inherited in successive plant generations (R 0 , R 1 and R 2 progeny) as shown by molecular analysis.A Western blot analysis of plant extracts showed the presence of a polypeptide of the expected size that cross-reacted with TSP14-specific antibodies. Larvae of the tobacco budworm (Heliothis virescens) and tobacco hornworm (Manduca sexta) which were fed with several independent homozygous transgenic plant lines (R 2 progeny) exhibited mortality and reduced growth rates compared to those fed with plants transformed by a vector control. Our results demonstrate the potential for introduction of the TSP14 gene into plants in order to achieve protection against lepidopteran pests.

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“…Cholesterol oxidase from Streptomyces is highly toxic to cotton boll weevil (Anthonomus grandis) (Cho et al, 1995), while polyphenol oxidases and peroxidases increase the inhibitory effect of 5CQA (5-caffeoyl quinic acid) and cholorogenic acid by oxidizing the dihydroxy groups to ubiquinones that covalently bind to nucleophilic (-SH2 and -NH2) groups of proteins, peptides, and amino acids. Mechanical wounding and insect damage resulted in transient increase in activity of polyphenol oxidase (Dhankher and Gatehouse, 2003).…”

Section: Enzymesmentioning

confidence: 99%

Genetic Transformation of Crops for Insect Resistance: Potential and Limitations

Sharma

Crouch

2004

Critical Reviews in Plant Sciences

129

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Transgenic resistance to insects has been demonstrated in plants expressing insecticidal genes such as δ-endotoxins from Bacillus thuringiensis (Bt), protease inhibitors, enzymes, secondary plant metabolites, and plant lectins. While transgenic plants with introduced Bt genes have been deployed in several crops on a global scale, the alternative genes have received considerably less attention. The protease inhibitor and lectin genes largely affect insect growth and development and, in most instances, do not result in insect mortality. The effective concentrations of these proteins are much greater than the Bt toxin proteins. Therefore, the potential of some of the alternative genes can only be realized by deploying them in combination with conventional host plant resistance and Bt genes. Genes conferring resistance to insects can also be deployed as multilines or synthetic varieties. Initial indications from deployment of transgenics with insect resistance in diverse cropping systems in USA, Africa suggest that single transgene products in standard cultivar backgrounds are not a recipe for sustainable pest management. Instead, a much more complex approach may be needed, one which may involve deployment of a combination of different transgenes in different backgrounds. Under diverse climatic conditions and cropping systems of tropics, the success in the utilization of transgenics for pest management may involve decentralized national breeding programs and several small-scale seed companies. While several transgenic crops with insecticidal genes have been introduced in the temperate regions, very little has been done to use this technology for improving crop productivity in the harsh environments of the tropics, where the need for increasing food production is most urgent. There is a need to develop appropriate strategies for deployment of transgenics for pest management, keeping in view the pest spectrum involved, and the effects on nontarget organisms in the ecosystem.

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Introduction and expression of the Streptomyces cholesterol oxidase gene (ChoA), a potent insecticidal protein active against boll weevil larvae, into tobacco cells

Cited by 35 publications

References 21 publications

Affinity purification of a cholesterol oxidase expressed in Escherichia coli

Affinity purification of a cholesterol oxidase expressed in Escherichia coli

Antibiosis‐type insect resistance in transgenic plants expressing a teratocyte secretory protein (TSP14) gene from a hymenopteran endoparasite (Microplitis croceipes)

Genetic Transformation of Crops for Insect Resistance: Potential and Limitations

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