Introduction of a xantha mutation for testing and increasing varietal purity in hybrid rice

Zhou, Xiang-sheng; Sheng-quan, Shen; Wu, Dianxing; Sun, Jian; Shu, Qingyao

doi:10.1016/j.fcr.2005.05.008

Cited by 28 publications

(29 citation statements)

References 4 publications

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“…Previous results already demonstrated that the xantha trait segregated as a single recessive gene in the populations derived from crosses between indica varieties (Zhou et al 2006). In this study, similar results were observed in the two japonica 9 indica populations (Table 1), indicating the full penetration of the mutated trait in different genetic backgrounds.…”

Section: Inheritance Of the Xantha Traitsupporting

confidence: 92%

“…Mgt-1 was used as the recurrent parent and backcrossed to II32 A, and a new CMS line Huangyu A was developed and Mgt-1 was named Huangyu B, serving as the maintainer line of Huangyu A (Zhou et al 2006). Although Mgt-1 was recorded as a mutant of the CMS maintainer line II32 B, it was later identified to be a mutant of another variety Longtefu B after molecular characterization using SSR markers (Fu et al 2007).…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, Longtefu B (wild type) is the progenitor of Huangyu B (xantha mutant). It has been proven that the xantha trait is under the control of a single recessive mutation and it can be used as a useful visual marker for testing and increasing varietal purity in hybrid rice production (Zhou et al 2006).…”

Section: Introductionmentioning

confidence: 99%

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Fine mapping of a Xantha mutation in rice (Oryza sativa L.)

2009

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The recessive mutation of the XANTHA gene (XNT) transforms seedlings and plants into a yellow color, visually distinguishable from normal (green) rice. Thus, it has been introduced into male sterile lines as a distinct marker for rapidly testing and efficiently increasing varietal purity in seed and paddy production of hybrid rice. To identify closely linked markers and eventually isolate the XNT gene, two mapping populations were developed by crossing the xantha mutant line Huangyu B (indica) with two wild type japonica varieties; a total of 1,720 mutant type F 2 individuals were analyzed for fine mapping using polymorphic InDel markers and high dense microsatellite markers. The XNT gene was mapped on chromosome 11, within in a fragment of *100 kb, where 13 genes are annotated. The NP_001067671.1 gene within the delimited region is likely to be a candidate XNT gene, since it encodes ATP-dependent chloroplast protease ATP-binding subunit clp A. However, no sequence differences were observed between the mutant and its parent. Bioinformatics analysis demonstrated that four chlorophyll deficient mutations that were previously mapped on the same chromosome are located outside the XNT region, indicating XNT is a new gene. The results provide useful DNA markers not only for marker assisted selection of the xantha trait but also its eventual cloning.

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Section: Inheritance Of the Xantha Traitsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

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Fine mapping of a Xantha mutation in rice (Oryza sativa L.)

2009

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“…Inspired by recent gene mapping studies (Chen et al 2006;Deng et al 2006;Li et al 2006), we have launched a fine mapping program for a series of mutants for important quality and agronomic traits in rice that have a similar pattern of inheritance to those of the genes in the above examples (Liu et al 2007;Zhou et al 2006). However, the mapping design, including random and/or nearly uniformly distributed marker selection in the existing procedures (Chen et al 2006;Deng et al 2006;Li et al 2006), was not optimal because meiotic recombination events are distributed unevenly throughout the genome (Drouaud et al 2006;Petes 2001;Wu et al 2003).…”

Section: Introductionmentioning

confidence: 96%

An optimal DNA pooling strategy for progressive fine mapping

et al. 2008

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We present a cost-effective DNA pooling strategy for fine mapping of a single Mendelian gene in controlled crosses. The theoretical argument suggests that it is potentially possible for a single-stage pooling approach to reduce the overall experimental expense considerably by balancing costs for genotyping and sample collection. Further, the genotyping burden can be reduced through multi-stage pooling. Numerical results are provided for practical guidelines. For example, the genotyping effort can be reduced to only a small fraction of that needed for individual genotyping at a small loss of estimation accuracy or at a cost of increasing sample sizes slightly when recombination rates are 0.5% or less. An optimal two-stage pooling scheme can reduce the amount of genotyping to 19.5%, 14.5% and 6.4% of individual genotyping efforts for identifying a gene within 1, 0.5, and 0.1 cM, respectively. Finally, we use a genetic data set for mapping the rice xl(t) gene to demonstrate the feasibility and efficiency of the DNA pooling strategy. Taken together, the results demonstrate that this DNA pooling strategy can greatly reduce the genotyping burden and the overall cost in fine mapping experiments.

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“…Currently, the transgenic or naturally mutated herbicide-resistant rice were applied for hybrid rice seed production. The herbicide-resistant transgenic rice was used as the pollen parent in hybrid rice seed production process and the false hybrid seeds could be eliminated by testing herbicide resistance in F 1 seedlings (Xiao et al 2005;Zhou et al 2006). Zhang et al (2002) found a herbicide (bentazon) sensitive mutant TGMS and the selfed seeds could be eliminated by spraying the herbicide bentazon at F 1 seedlings.…”

Section: Introductionmentioning

confidence: 99%

Use of herbicide-resistant genic male sterility in hybrid rice seed production

et al. 2007

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One of the difficult problems in hybrid rice seed production is the low outcrossing frequency and requirement for much labor to produce hybrid seeds. In order to simplify the process of hybrid rice seed production, herbicide-resistant photoperiod sensitive genic male sterile (HRPGMS) rice was utilized in this study. The herbicide resistance gene bar was transferred into the photoperiod sensitive genic male sterile (PGMS) rice 920S by Agrobacterium-mediated transformation and the HRPGMS line YA3530ms with good agronomic characteristics was bred by applying conventional pedigree breeding technique. The seeds of HRPGMS and pollen parent were mixed with the ratio of 4:1 in weight, and were sowed in seedling box. The mixed seedlings of HRPGMS and pollen parent grown for 30 days were transplanted by the small transplanting machine in the field. The herbicide glufosinate ammonium was sprayed at 7 days after flowering to kill all the plants of pollen parent, whereas hybrid seeds were harvested from the survived HRPGMS parent at maturity. The outcrossing frequency of HRPGMS line from two combinations in 2002 and from five combinations in 2004 were compared with a control cultivated by the conventional 2-line system. As the result, the mean outcrossing frequency in HRPGMS of the treatments were 10.6-24.5% compared with 5.5% in PGMS of the control in 2002, and that were 24.7-32.0% compared with 7.5% in the control in 2004. Consequently, using HRPGMS in two-line system was proved to be a new method that would simplify the process of hybrid rice seed production and to increase outcrossing frequency without any artificial supplementary pollination processes.

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Introduction of a xantha mutation for testing and increasing varietal purity in hybrid rice

Cited by 28 publications

References 4 publications

Fine mapping of a Xantha mutation in rice (Oryza sativa L.)

Fine mapping of a Xantha mutation in rice (Oryza sativa L.)

An optimal DNA pooling strategy for progressive fine mapping

Use of herbicide-resistant genic male sterility in hybrid rice seed production

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