Introduction of the ZmDof1 gene into rice enhances carbon and nitrogen assimilation under low‐nitrogen conditions

Kurai, Tomohiro; Wakayama, Masataka; Abiko, Tomomi; Yanagisawa, Shuichi; Aoki, Naohiro; Ohsugi, Ryu

doi:10.1111/j.1467-7652.2011.00592.x

Cited by 183 publications

(126 citation statements)

References 40 publications

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“…Recently, ZmDof1-overexpressing experiments in rice showed enhanced N and C accumulation and photosynthesis rates in transgenic rice plants under N-limiting conditions. N accumulation occurred particularly in roots that had also a higher biomass than the control plants ( Kurai et al, 2011).…”

Section: Genetic Modifications Involving Transcription Factors and Otmentioning

confidence: 99%

Biotechnology of nutrient uptake and assimilation in plants

et al. 2013

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Plants require a complex balance of mineral nutrients to reproduce successfully. Because the availability of many of these nutrients in the soil is compromised by several factors, such as soil pH, cation presence, and microbial activity, crop plants depend directly on nutrients applied as fertilizers to achieve high yields. However, the excessive use of fertilizers is a major environmental concern due to nutrient leaching that causes water eutrophication and promotes toxic algae blooms. This situation generates the urgent need for crop plants with increased nutrient use efficiency and better-designed fertilization schemes. The plant biology revolution triggered by the development of efficient gene transfer systems for plant cells together with the more recent development of next-generation DNA and RNA sequencing and other omics platforms have advanced considerably our understanding on the molecular basis of plant nutrition and how plants respond to nutritional stress. To date, genes encoding sensors, transcription factors, transporters, and metabolic enzymes have been identified as potential candidates to improve nutrient use efficiency. In addition, the study of other genetic resources, such as bacteria and fungi, allows the identification of alternative mechanisms of nutrient assimilation, which are potentially applicable in plants. Although significant progress in this respect has been achieved by conventional breeding, in this review we focus on the biotechnological approaches reported to date aimed at boosting the use of the three most limiting nutrients in the majority of arable lands: nitrogen, phosphorus, and iron.

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Section: Genetic Modifications Involving Transcription Factors and Otmentioning

confidence: 99%

Biotechnology of nutrient uptake and assimilation in plants

et al. 2013

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“…For example, the plant-specific transcription factor Dof zinc finger protein1 from maize (Zea mays) can increase the expression of phosphoenolpyruvate carboxylase and several genes involved in the tricarboxylic acid cycle, which results in the production of more carbon skeletons for the assimilation of N in Arabidopsis (Arabidopsis thaliana; Yanagisawa et al, 2004). This transcription factor has been used successfully to improve N use efficiency and enhance growth in rice under low-N conditions by increasing carbon flow toward N assimilation (Kurai et al, 2011). The MYB-CC-type transcription factor PHOSPHATE STARVA-TION RESPONSE1 (PHR1) plays a central role in the signaling network for sensing P availability (Rubio et al, 2001;Chiou and Lin, 2011).…”

mentioning

confidence: 99%

A Wheat CCAAT Box-Binding Transcription Factor Increases the Grain Yield of Wheat with Less Fertilizer Input

Qu¹,

He²,

Wang

et al. 2014

Plant Physiology

164

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Increasing fertilizer consumption has led to low fertilizer use efficiency and environmental problems. Identifying nutrient-efficient genes will facilitate the breeding of crops with improved fertilizer use efficiency. This research performed a genome-wide sequence analysis of the A (NFYA), B (NFYB), and C (NFYC) subunits of Nuclear Factor Y (NF-Y) in wheat (Triticum aestivum) and further investigated their responses to nitrogen and phosphorus availability in wheat seedlings. Sequence mining together with gene cloning identified 18 NFYAs, 34 NFYBs, and 28 NFYCs. The expression of most NFYAs positively responded to low nitrogen and phosphorus availability. In contrast, microRNA169 negatively responded to low nitrogen and phosphorus availability and degraded NFYAs. Overexpressing TaNFYA-B1, a low-nitrogen- and low-phosphorus-inducible NFYA transcript factor on chromosome 6B, significantly increased both nitrogen and phosphorus uptake and grain yield under differing nitrogen and phosphorus supply levels in a field experiment. The increased nitrogen and phosphorus uptake may have resulted from the fact that that overexpressing TaNFYA-B1 stimulated root development and up-regulated the expression of both nitrate and phosphate transporters in roots. Our results suggest that TaNFYA-B1 plays essential roles in root development and in nitrogen and phosphorus usage in wheat. Furthermore, our results provide new knowledge and valuable gene resources that should be useful in efforts to breed crops targeting high yield with less fertilizer input.

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“…The plant-specific DNA BINDING WITH ONE FINGER (Dof) TF Dof1 from maize (Zea mays) can increase the expression of PHOSPHOENOLPYRUVATE CARBOXYLASE and several genes involved in the tricarboxylic acid cycle and thereby produce more carbon skeletons for the assimilation of N in Arabidopsis (Arabidopsis thaliana; Yanagisawa et al, 2004). This TF has been used successfully to increase the net photosynthesis rate and to increase carbon flow toward N assimilation and, consequently, to improve N assimilation and growth of rice under low-N conditions (Kurai et al, 2011).…”

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confidence: 99%

The nitrate inducible NAC transcription factor TaNAC2-5A controls nitrate response and increases wheat yield

et al. 2015

Plant Physiol.

123

143

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Nitrate is a major nitrogen resource for cereal crops; thus, understanding nitrate signaling in cereal crops is valuable for engineering crops with improved nitrogen use efficiency. Although several regulators have been identified in nitrate sensing and signaling in Arabidopsis (Arabidopsis thaliana), the equivalent information in cereals is missing. Here, we isolated a nitrateinducible and cereal-specific NAM, ATAF, and CUC (NAC) transcription factor, TaNAC2-5A, from wheat (Triticum aestivum). A chromatin immunoprecipitation assay showed that TaNAC2-5A could directly bind to the promoter regions of the genes encoding nitrate transporter and glutamine synthetase. Overexpression of TaNAC2-5A in wheat enhanced root growth and nitrate influx rate and, hence, increased the root's ability to acquire nitrogen. Furthermore, we found that TaNAC2-5A-overexpressing transgenic wheat lines had higher grain yield and higher nitrogen accumulation in aerial parts and allocated more nitrogen in grains in a field experiment. These results suggest that TaNAC2-5A is involved in nitrate signaling and show that it is an exciting gene resource for breeding crops with more efficient use of fertilizer.

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Introduction of the ZmDof1 gene into rice enhances carbon and nitrogen assimilation under low‐nitrogen conditions

Cited by 183 publications

References 40 publications

Biotechnology of nutrient uptake and assimilation in plants

Biotechnology of nutrient uptake and assimilation in plants

A Wheat CCAAT Box-Binding Transcription Factor Increases the Grain Yield of Wheat with Less Fertilizer Input

The nitrate inducible NAC transcription factor TaNAC2-5A controls nitrate response and increases wheat yield

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