Introduction: Review of microscopic studies on the fetal and neonatal kidney

Dodge, Alice H.

doi:10.1002/(sici)1097-0029(19971101)39:3<205::aid-jemt1>3.0.co;2-j

Cited by 12 publications

(6 citation statements)

References 45 publications

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“…In rats, the kidney is not fully developed at birth (Dodge, 1997;Kahane et al, 1995;Márquez et al, 2002). We have previously reported that, until postnatal Day 10, most renal papillary cells are found in different phases of the cell cycle, many of them have an embryonic phenotype (Kahane et al, 1995;Márquez et al, 2002), and blind ends of CD branches underneath the capsule are still present (Márquez et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

“…As a result, the UB epithelium undergoes branching morphogenesis to give rise to the CD system of the adult kidney, whereas the metanephric mesenchyme gives rise to the nephron (Aperia & Celsi, ; Dressler, ; Pohl, Stuart, & Nigam, ; Saxen, ). In humans, nephrogenesis is completed in utero before week 36, whereas in rats, it is completed postnatally (Aperia & Celsi, ; Dodge, ; Márquez, Cabrera, Serrano, & Sterin‐Speziale, ). In previous studies, we and others have demonstrated that, at birth, the rat kidney is very immature, and that all stages of renal glomerular and tubular development can be visualized in histological sections of the newborn rat kidney (Dodge, ; Márquez et al, ).…”

Section: Introductionmentioning

confidence: 99%

“…In humans, nephrogenesis is completed in utero before week 36, whereas in rats, it is completed postnatally (Aperia & Celsi, ; Dodge, ; Márquez, Cabrera, Serrano, & Sterin‐Speziale, ). In previous studies, we and others have demonstrated that, at birth, the rat kidney is very immature, and that all stages of renal glomerular and tubular development can be visualized in histological sections of the newborn rat kidney (Dodge, ; Márquez et al, ). We have also demonstrated that the postnatal maturation is different in various areas of the kidney (Kahane et al, ; Márquez et al, ) and that different cell types also show a different degree of proliferation in each kidney area.…”

Section: Introductionmentioning

confidence: 99%

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Bradykinin mediates the association of collecting duct cells to form migratory colonies, through B2 receptor activation

Guaytima

Brandán

Favale

et al. 2018

Journal Cellular Physiology

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It is known that bradykinin (BK) B2 receptor (B2R) is expressed in the collecting duct (CD) cells of the newborn rat kidney, but little is known about its role during early postnatal life. Therefore, we hypothesize that BK could participate in the mechanisms that mediate CD formation during the postnatal renal development. Performing primary cultures, combined with biochemical, immunocytochemical, and time-lapse analysis, we studied the role of BK in CD cell behavior isolated from renal papilla of neonatal rats. A reverse relationship was observed between B2R expression and the degree of CD epithelial cell sheet maturation. BK stimulation induced CD cell association upon B2R activation. The lack of B2R expression in cells showing mature adherens junctions suggested that BK is mostly involved in early adhesive events, thus favoring the initial formation of CD during development. Time-lapse analysis revealed that BK induced a high protrusive activity of CD cells, denoted by ruffle formation and lamellipodia extension. PI3K was involved in the BK-induced CD cell-cell association and the acquisition of the migratory phenotype since, when inhibited, membrane ruffles, and filopodia between cells diminished. Results indicate that the actions of BK mediated by PI3K activation were due to the downstream Akt and Rac pathways. This study, performed with CD cells that were not genetically manipulated, provides new experimental evidence supporting a novel role of BK in rat renal CD organization. As B2R blockade results in abnormal tubular differentiation, our results contribute to better understanding the etiology of human congenital renal malformation and diseases.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Bradykinin mediates the association of collecting duct cells to form migratory colonies, through B2 receptor activation

Guaytima

Brandán

Favale

et al. 2018

Journal Cellular Physiology

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“…The kidneys (K), shown at level 12 ( Figure 6), are very immature yet have initiated nephrogenesis. The cortex can be divided into a basophilic subcapsular nephrogenic zone, the juxtamedullary zone, and the intercortical zone (Dodge 1997). The subcapsular nephrogenic zone includes the 2 earliest stages of developing nephrons including vesicles (stage 1) and S-shaped structures (stage 2), while the juxtamedullary zone contains immature glomeruli (stage 3).…”

Section: The Rat Fetusmentioning

confidence: 99%

Histomorphologic Analysis of the Late-term Rat Fetus and Placenta

et al. 2018

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Histological examination of the rat placenta and fetus is uncommon. Toxicological studies mainly rely on gross examination of the fetus and on fetal and placental weights. These are often insufficient to assess the fetal and placental toxicity of xenobiotics. The small size of the fetus makes its dissection labor-intensive. Thus, our objective was to develop a simple and accurate technique to evaluate the rat fetus and placenta. Sprague-Dawley rat fetuses at gestational day 19.5 ( n = 18) and their placentas ( n = 32) were fixed in formalin. Placentas were cut transversally in the center. Fetuses were cut following a freehand whole-body serial sectioning diagram adapted from Wilson's method. Sections were stained with hematoxylin-eosin-phloxine-saffron, and histomorphometry was used to measure the area of the fetal placental region (27.2 ± 1.7 mm), including the labyrinth (22.2 ± 1.0 mm) and the basal zone (4.8 ± 0.8 mm). Our whole-fetus serial sectioning technique resulted in 12 precise cutting planes that fit on 3 histological slides, enabling the examination of most organs without labor-intensive dissection. Quantitative analysis of placental areas improves the understanding of the pathogenesis of treatment-related changes. This technique provides a standardized method for future research in pertinent fields such as developmental biology and toxicology.

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“…Em camundongos, a nefrogênese dos rins definitivos começa entre os 11 e 12 dias de vida intrauterina e se completa entre 7 a 10 dias após o nascimento (Speller & Moffat, 1977;Bush et al, 2004). Desta forma, em cortes histológicos do rim de camundongos a termo podem ser visualizados todos os estágios de desenvolvimento glomerular e tubular (Mugrauer & Ekblom, 1991;Dodge, 1997 desenvolvidos estão no processo final de diferenciação.…”

Section: Vulnerabilidade Fetal à Poluição Atmosféricaunclassified

Estudo estereológico do efeito da exposição gestacional à poluição ambiental de São Paulo sobre o desenvolvimento renal em camundongos

Rodrigues¹

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Introduction: Review of microscopic studies on the fetal and neonatal kidney

Cited by 12 publications

References 45 publications

Bradykinin mediates the association of collecting duct cells to form migratory colonies, through B2 receptor activation

Bradykinin mediates the association of collecting duct cells to form migratory colonies, through B2 receptor activation

Histomorphologic Analysis of the Late-term Rat Fetus and Placenta

Estudo estereológico do efeito da exposição gestacional à poluição ambiental de São Paulo sobre o desenvolvimento renal em camundongos

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