Introgression of bacterial wilt resistance from Solanum melongena to S . t uberosum through asymmetric protoplast fusion

Liu, Ting; Yu, Yan; Cai, Xingkui; Tu, Wei; Xie, Chao; Liu, Jun

doi:10.1007/s11240-016-0958-9

Cited by 18 publications

(17 citation statements)

References 48 publications

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“…R. solanacearum is able to persist in soil, weed, plant debris, rhizospheres, and alternate hosts. It can be spreaded trough irrigation water or infected planting material hindering pathogen eradication ( Hayward, 1991 ; Laferriere et al, 1999 ; Liu et al, 2016 ). When living freely in the soil, R. solanacearum occurs in a motile form to efficiently find and invade the host which locates through chemotaxis and aerotaxis ( Yao and Allen, 2006 , 2007 ).…”

Section: Introductionmentioning

confidence: 99%

“…Potato ( Solanum tuberosum ) is widely cultivated across the world, and is regarded as the fourth most important food crop and one of the most significant solanaceous crops ( Liu et al, 2016 ). For potato grown in the tropics, BW is one of the most important biotic constraints to production after late blight caused by the oomycete Phytophthora infestans ( Priou et al, 1999 ).…”

Section: Introductionmentioning

confidence: 99%

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Enhanced Bacterial Wilt Resistance in Potato Through Expression of Arabidopsis EFR and Introgression of Quantitative Resistance from Solanum commersonii

Boschi¹,

Schvartzman

Murchio

et al. 2017

Front. Plant Sci.

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Bacterial wilt (BW) caused by Ralstonia solanacearum is responsible for substantial losses in cultivated potato (Solanum tuberosum) crops worldwide. Resistance genes have been identified in wild species; however, introduction of these through classical breeding has achieved only partial resistance, which has been linked to poor agronomic performance. The Arabidopsis thaliana (At) pattern recognition receptor elongation factor-Tu (EF-Tu) receptor (EFR) recognizes the bacterial pathogen-associated molecular pattern EF-Tu (and its derived peptide elf18) to confer anti-bacterial immunity. Previous work has shown that transfer of AtEFR into tomato confers increased resistance to R. solanacearum. Here, we evaluated whether the transgenic expression of AtEFR would similarly increase BW resistance in a commercial potato line (INIA Iporá), as well as in a breeding potato line (09509.6) in which quantitative resistance has been introgressed from the wild potato relative Solanum commersonii. Resistance to R. solanacearum was evaluated by damaged root inoculation under controlled conditions. Both INIA Iporá and 09509.6 potato lines expressing AtEFR showed greater resistance to R. solanacearum, with no detectable bacteria in tubers evaluated by multiplex-PCR and plate counting. Notably, AtEFR expression and the introgression of quantitative resistance from S. commersonii had a significant additive effect in 09509.6-AtEFR lines. These results show that the combination of heterologous expression of AtEFR with quantitative resistance introgressed from wild relatives is a promising strategy to develop BW resistance in potato.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhanced Bacterial Wilt Resistance in Potato Through Expression of Arabidopsis EFR and Introgression of Quantitative Resistance from Solanum commersonii

Boschi¹,

Schvartzman

Murchio

et al. 2017

Front. Plant Sci.

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“…Somatic hybrids may exhibit unique and useful characteristics derived from the combination of two parental components. Hence, novel plants with altered phenotypes and increased tolerance to biotic and abiotic stress have been created 12 – 14 . Only a few reports concern somatic hybridisation in the carrot.…”

Section: Introductionmentioning

confidence: 99%

Real-time detection of somatic hybrid cells during electrofusion of carrot protoplasts with stably labelled mitochondria

Gieniec

Siwek

Oleszkiewicz

et al. 2020

Sci Rep

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Somatic hybridisation in the carrot, as in other plant species, enables the development of novel plants with unique characteristics. This process can be induced by the application of electric current to isolated protoplasts, but such electrofusion requires an effective hybrid cell identification method. This paper describes the non-toxic fluorescent protein (FP) tagging of protoplasts which allows discrimination of fusion components and identification of hybrids in real-time during electrofusion. One of four FPs: cyan (eCFP), green (sGFP), yellow (eYFP) or the mCherry variant of red FP (RFP), with a fused mitochondrial targeting sequence, was introduced to carrot cell lines of three varieties using Agrobacterium-mediated transformation. After selection, a set of carrot callus lines with either GFP, YFP or RFP-labelled mitochondria that showed stable fluorescence served as protoplast sources. Various combinations of direct current (DC) parameters on protoplast integrity and their ability to form hybrid cells were assessed during electrofusion. The protoplast response and hybrid cell formation depended on DC voltage and pulse time, and varied among protoplast sources. Heterofusants (GFP + RFP or YFP + RFP) were identified by detection of a dual-colour fluorescence. This approach enabled, for the first time, a comprehensive assessment of the carrot protoplast response to the applied electric field conditions as well as identification of the DC parameters suitable for hybrid formation, and an estimation of the electrofusion success rate by performing real-time observations of protoplast fluorescence.

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“…The pathogen can alternate hosts and persist, spread, and survive in different natural habitats, including soil, weed, plant debris, rhizospheres. In addition, it can be spread through irrigation water or infected planting material hindering disease control (7)(8) (9) . This set of characteristics makes it very challenging to achieve a sustainable management of the disease (10) .…”

Section: Introductionmentioning

confidence: 99%

Potato plants transformed with the Arabidopsis EF-Tu receptor (EFR) show restricted pathogen colonization and enhanced bacterial wilt resistance under conditions resembling natural field infections

et al. 2020

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Potato is considered a staple food in the world and its production is limited by the presence of bacterial wilt (bw) disease caused by Ralstonia solanacearum. Host resistance is the most sustainable and cost-effective strategy to manage bw, although resistant commercial potato cultivars are not yet available. Our group incorporated the efr receptor of Arabidopsis thaliana (atefr), which recognizes the elongation factor Tu, preserved in bacteria, triggering an immune response. atefr was tested in two genetic backgrounds: a susceptible commercial cultivar (inia Iporá) and a breeding clone with partial resistance introgressed from Solanum commersonii. In this work, the effect of the atefr receptor on bw resistance was evaluated for the first time, using conditions resembling natural field infection. In addition, the colonization patterns of wild-type and transgenic lines were compared by using luminescent and fluorescent R. solanacearum reporter strains. Both approaches showed a delay and a decrease in the severity of wilting symptoms in the atefr-transformed genotypes. Differential colonization patterns were observed, revealing a higher bacterial development in the non-transformed plants. This atefr effect seems more pronounced in the interspecific breeding line, possibly leading to a more effective activation of the plant immune system.

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Introgression of bacterial wilt resistance from Solanum melongena to S . t uberosum through asymmetric protoplast fusion

Cited by 18 publications

References 48 publications

Enhanced Bacterial Wilt Resistance in Potato Through Expression of Arabidopsis EFR and Introgression of Quantitative Resistance from Solanum commersonii

Enhanced Bacterial Wilt Resistance in Potato Through Expression of Arabidopsis EFR and Introgression of Quantitative Resistance from Solanum commersonii

Real-time detection of somatic hybrid cells during electrofusion of carrot protoplasts with stably labelled mitochondria

Potato plants transformed with the Arabidopsis EF-Tu receptor (EFR) show restricted pathogen colonization and enhanced bacterial wilt resistance under conditions resembling natural field infections

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