Introgression of cotton leaf curl virus-resistant genes from Asiatic cotton (Gossypium arboreum) into upland cotton (G. hirsutum)

Ahmad, Saghir; Mahmood, Khalid; Hanif, Mamoona; Nazeer, Wajad; Malik, Waqas; Qayyum, Abdul; Hanif, Kanwal; Mahmood, Abid; Islam, Nusrat Ul

doi:10.4238/2011.october.7.2

Cited by 23 publications

(16 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…G. hirsutum and G. barbadense have chromosome homology and the tetraploid genomes, are not separated by any large scale chromosomal rearrangement [53]. However, crosses between G. hirsutum and G. arboreum L. are rarely successful without hormone application [44], [54]. Plant hormones are known to control pollen tube growth [55].…”

Section: Discussionmentioning

confidence: 99%

“…We observed very wide genetic variability among BC 1 to BC 3 progenies, which will certainly facilitate improvement of cotton resistances to diseases. And various scientists also utilized G. arboreum L. for introgression of desirable resistant genes into cultivated tetraploid cotton for drought [34]–[35], heat [36], root rot, cotton leaf curl virus [37,[44] and insect pests (bollworms and aphids) [12]. Therefore, the introgression lines of G. arboreum developed with or wothout resistance in this study can be employed to map the resistance gene(s)/loci, which will be very useful for future diverse (a)biotic-tolerant cotton breeding.…”

Section: Discussionmentioning

confidence: 99%

“…The F 1 CLCuD-resistant progeny involving these parents, which was developed by Ahmad et al [44], comprising direct cross [2( G. arboreum )×G. hirsutum ] and its reciprocal cross ( G. hirsutum × G. arboreum ), was utilized to directly backcross with G. hirsutum .…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of Cotton Leaf Curl Virus Resistance in BC1, BC2, and BC3 Progenies from an Interspecific Cross between Gossypium arboreum and Gossypium hirsutum

et al. 2014

View full text Add to dashboard Cite

Cotton leaf curl virus disease (CLCuD) is an important constraint to cotton production. The resistance of G. arboreum to this devastating disease is well documented. In the present investigation, we explored the possibility of transferring genes for resistance to CLCuD from G. arboreum (2n = 26) cv 15-Mollisoni into G. hirsutum (2n = 52) cv CRSM-38 through conventional breeding. We investigated the cytology of the BC1 to BC3 progenies of direct and reciprocal crosses of G. arboreum and G. hirsutum and evaluated their resistance to CLCuD. The F1 progenies were completely resistant to this disease, while a decrease in resistance was observed in all backcross generations. As backcrossing progressed, the disease incidence increased in BC1 (1.7–2.0%), BC2 (1.8–4.0%), and BC3 (4.2–7.0%). However, the disease incidence was much lower than that of the check variety CIM-496, with a CLCuD incidence of 96%. Additionally, the disease incidence percentage was lower in the direct cross 2(G. arboreum)×G. hirsutum than in that of G. hirsutum×G. arboreum. Phenotypic resemblance of BC1 ∼BC3 progenies to G. arboreum confirmed the success of cross between the two species. Cytological studies of CLCuD-resistant plants revealed that the frequency of univalents and multivalents was high in BC1, with sterile or partially fertile plants, but low in BC2 (in both combinations), with shy bearing plants. In BC3, most of the plants exhibited normal bearing ability due to the high frequency of chromosome associations (bivalents). The assessment of CLCuD through grafting showed that the BC1 to BC3 progenies were highly resistant to this disease. Thus, this study successfully demonstrates the possibility of introgressing CLCuD resistance genes from G. arboreum to G. hirsutum.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of Cotton Leaf Curl Virus Resistance in BC1, BC2, and BC3 Progenies from an Interspecific Cross between Gossypium arboreum and Gossypium hirsutum

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Ansari (1958) reported that the plants of G. stocksii were free of pests and diseases and speculated on the possibility of the usefulness of this species in producing disease-resistant cultivated cotton by hybridization. Ahmad et al (2011) found G. stocksii resistant to cotton leaf curl virus disease. This species is found in the desert area of Sindh, Pakistan, Arabia, and East Africa.…”

Section: Introductionmentioning

confidence: 99%

“…It is worthwhile to combine the genes for cotton leaf curl virus resistance and other diseases and drought resistance between Gossypium hirsutum L. and Gossypium arboreum L. cotton (Amin, 1940). Moreover, other researchers (Bao-Liang et al, 2003;Ahmad et al, 2011) have been successful in interspecific introgression of Gossypium hirsutum L. and Gossypium arboreum L. Similarly, Knight (1957) and Brinkerhoff (1970) introgressed resistant genes B 6 found in the 'A' genome of Gossypium arboreum L. against bacterial blight caused by Xanthomonas malvacearum into Gossypium barbadense L. Breeders have also achieved developing the most resistant commercial variety 'Auburn 56' to root knot nematode (Meloidogyne incognita) in U.S. cotton through transgressive segregation (Shepherd, 1974). Sacks and Robinson (2009) introgressed resistance to Rotylenchulus reniformis into the tetraploid 2 (AD) 1 through crossing a resistant diploid A 2 -genome Gossypium arboreum L. accession (A 2 -190) with a hexaploid 2 [(AD) 1 D 4 ] bridging line (G 371) to obtain a tetraploid triple species hybrid.…”

Section: Introductionmentioning

confidence: 99%

Introgression of genes for cotton leaf curl virus resistance and increased fiber strength from Gossypium stocksii into upland cotton (G. hirsutum)

Nazeer¹,

Ahmad²,

Mahmood³

et al. 2014

Genet. Mol. Res.

Self Cite

View full text Add to dashboard Cite

ABSTRACT. Cotton leaf curl virus disease is a major hurdle for successful cotton production in Pakistan. There has been considerable economic loss due to this disease during the last decade. It would be desirable to have cotton varieties resistant to this disease. We explored the possibility of transferring virus resistant genes from the wild species Gossypium stocksii into MNH-786, a cultivar of G. hirsutum. Hybridization was done under field condition at the Cotton Research Station, Multan, during 2010-11. Boll shedding was controlled by application of exogenous hormones. F 1 seeds were treated with 0.03% colchicine solution for 6 h and germinated. Cytological observations at peak squaring/flowering stage showed that these plants were hexaploid, having 2n = 6x = 78 chromosomes. The and size, bracteole area, bracteole dentation, flower size, pedicel size, and petal number and size. Moreover it possessed high fiber strength of 54.4 g/tex, which is 54% greater than that of the check variety, i.e. . The F 1 population did not show any symptom of CLCuVD in the field, tested by grafting with CLCuVD susceptible rootstock (var. S12). We conclude that it is possible to transfer CLCuVD resistance and high fiber strength from G. stocksii to G. hirsutum.

show abstract

Fiber Crops

Singh¹

2017

Wild Relatives of Cultivated Plants in India

View full text Add to dashboard Cite

Introgression of cotton leaf curl virus-resistant genes from Asiatic cotton (Gossypium arboreum) into upland cotton (G. hirsutum)

Cited by 23 publications

References 15 publications

Evaluation of Cotton Leaf Curl Virus Resistance in BC1, BC2, and BC3 Progenies from an Interspecific Cross between Gossypium arboreum and Gossypium hirsutum

Evaluation of Cotton Leaf Curl Virus Resistance in BC1, BC2, and BC3 Progenies from an Interspecific Cross between Gossypium arboreum and Gossypium hirsutum

Introgression of genes for cotton leaf curl virus resistance and increased fiber strength from Gossypium stocksii into upland cotton (G. hirsutum)

Fiber Crops

Contact Info

Product

Resources

About