Introgression of dual abiotic stress tolerance QTLs (Saltol QTL and Sub1 gene) into Rice (Oryza sativa L.) variety Aiswarya through marker assisted backcross breeding

Nair, Meenu M; Shylaraj, K. S.

doi:10.1007/s12298-020-00893-0

Cited by 24 publications

(5 citation statements)

References 19 publications

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“…(Padmavathi et al, 2023). The tightly linked markers within the Saltol QTL region (AP3206, RM8094, and RM3412), flanking markers, i.e., RM1287 and RM10694, RM493 and RM10793 enabled its successful transfer (Thomson et al, 2010) in the genetic background of BT7 (Linh et al, 2012), AS996 (Huyen et al, 2012), Bacthom 7 (Vu et al, 2012) in Vietnam, BRRI Dhan 49 (Hoque et al, 2015) in Bangladesh, Novator (Usatov et al, 2015) in Russia; ADT43 (Geetha et al, 2017), PB 1121 (Babu et al, 2017), Pusa Basmati 1 (Singh et al, 2018), Yukinko-mai (Rana et al, 2019), Pusa44 and Sarjoo 52 (Krishnamurthy et al, 2020), Pusa Basmati 1509 (Yadav et al, 2020), Aiswarya (Nair and Shylaraj, 2021) and Improved Samba Mahsuri (Rekha et al, 2022) in India. These MAS-derived varieties are already available to farmers for cultivation purposes.…”

Section: Molecular Breedingmentioning

confidence: 99%

Progress and prospects in harnessing wild relatives for genetic enhancement of salt tolerance in rice

Padmavathi,

Bangale,

Rao

et al. 2024

Front. Plant Sci.

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Salt stress is the second most devastating abiotic stress after drought and limits rice production globally. Genetic enhancement of salinity tolerance is a promising and cost-effective approach to achieve yield gains in salt-affected areas. Breeding for salinity tolerance is challenging because of the genetic complexity of the response of rice plants to salt stress, as it is governed by minor genes with low heritability and high G × E interactions. The involvement of numerous physiological and biochemical factors further complicates this complexity. The intensive selection and breeding efforts targeted towards the improvement of yield in the green-revolution era inadvertently resulted in the gradual disappearance of the loci governing salinity tolerance and a significant reduction in genetic variability among cultivars. The limited utilization of genetic resources and narrow genetic base of improved cultivars have resulted in a plateau in response to salinity tolerance in modern cultivars. Wild species are an excellent genetic resource for broadening the genetic base of domesticated rice. Exploiting novel genes of underutilized wild rice relatives to restore salinity tolerance loci eliminated during domestication can result in significant genetic gain in rice cultivars. Wild species of rice, Oryza rufipogon and Oryza nivara, have been harnessed in the development of a few improved rice varieties like Jarava and Chinsura Nona 2. Furthermore, increased access to sequence information and enhanced knowledge about the genomics of salinity tolerance in wild relatives has provided an opportunity for the deployment of wild rice accessions in breeding programs, while overcoming the cross-incompatibility and linkage drag barriers witnessed in wild hybridization. Pre-breeding is another avenue for building material that are ready for utilization in breeding programs. Efforts should be directed towards systematic collection, evaluation, characterization, and deciphering salt tolerance mechanisms in wild rice introgression lines and deploying untapped novel loci to improve salinity tolerance in rice cultivars. This review highlights the potential of wild relatives of Oryza to enhance tolerance to salinity, track the progress of work, and provide a perspective for future research.

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Section: Molecular Breedingmentioning

confidence: 99%

Progress and prospects in harnessing wild relatives for genetic enhancement of salt tolerance in rice

Padmavathi,

Bangale,

Rao

et al. 2024

Front. Plant Sci.

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“…Several reports of Saltol QTL introgression into high yielding indica rice varieties like IR64, BR11, BRRI Dhan 28, as well as Basmati rice varieties, indicate improvement of salinity tolerance at the seedling stage (Bimpong et al 2016;Babu et al 2017;Geetha et al 2017;Gregorio et al 2013;Guo and Ye 2014;Hasan et al 2015;Huyen et al 2012Huyen et al , 2013Krishnamurthy et al 2020;Nair and Shylaraj 2021;Yadav et al 2020). Conversely, to our knowledge only in a very recent study (Bundó et al 2022) the effects of the Saltol QTL introgression into japonica varieties have been evaluated.…”

Section: Introductionmentioning

confidence: 97%

Marker-Assisted Introgression of the Salinity Tolerance Locus Saltol in Temperate Japonica Rice

Marè¹,

Zampieri

Cavallaro

et al. 2023

Rice

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Background Rice is one of the most salt sensitive crops at seedling, early vegetative and reproductive stages. Varieties with salinity tolerance at seedling stage promote an efficient growth at early stages in salt affected soils, leading to healthy vegetative growth that protects crop yield. Saltol major QTL confers capacity to young rice plants growing under salt condition by maintaining a low Na+/K+ molar ratio in the shoots. Results Marker-assisted backcross (MABC) procedure was adopted to transfer Saltol locus conferring salt tolerance at seedling stage from donor indica IR64-Saltol to two temperate japonica varieties, Vialone Nano and Onice. Forward and background selections were accomplished using polymorphic KASP markers and a final evaluation of genetic background recovery of the selected lines was conducted using 15,580 SNP markers obtained from Genotyping by Sequencing. Three MABC generations followed by two selfing, allowed the identification of introgression lines achieving a recovery of the recurrent parent (RP) genome up to 100% (based on KASP markers) or 98.97% (based on GBS). Lines with highest RP genome recovery (RPGR) were evaluated for agronomical-phenological traits in field under non-salinized conditions. VN1, VN4, O1 lines were selected considering the agronomic evaluations and the RPGR% results as the most interesting for commercial exploitation. A physiological characterization was conducted by evaluating salt tolerance under hydroponic conditions. The selected lines showed lower standard evaluation system (SES) scores: 62% of VN4, and 57% of O1 plants reaching SES 3 or SES 5 respectively, while only 40% of Vialone Nano and 25% of Onice plants recorded scores from 3 to 5, respectively. VN1, VN4 and O1 showed a reduced electrolyte leakage values, and limited negative effects on relative water content and shoot/root fresh weight ratio. Conclusion The Saltol locus was successfully transferred to two elite varieties by MABC in a time frame of three years. The application of background selection until BC3F3 allowed the selection of lines with a RPGR up to 98.97%. Physiological evaluations for the selected lines indicate an improved salinity tolerance at seedling stage. The results supported the effectiveness of the Saltol locus in temperate japonica and of the MABC procedure for recovering of the RP favorable traits.

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“…Genomic regions responding to adaptive intonations in rice under submergence have been mapped, validated and successfully deployed in several intolerant genotypes [24][25][26][27][28][29][30][31][32]. The Sub1 gene exerts two weeks of submergence tolerance in mapped on chromosome-9, along with other minor QTLs in the Indian rice landrace FR13A [33][34][35][36][37].…”

Section: Introductionmentioning

confidence: 99%

“…FR13A is utilized as a donor of Sub1 in several rice improvement programs [4,9,28]. Popular rice varieties, Ranjit et al [24], BRRI Dhan52 [25], Aiswarya et al [27], Swarna et al [28], CO 3 [30], IR64 [32], Chehrang [32] and CR 1009 [32], etc., have been introgressed with Sub1 through the markers-assisted selection (MAS)/MAB approach. A submergence tolerant version of mega rice variety, Swarna Sub1 [28], has similar agro-morphology as Swarna, yielding a 2-fold or higher yield advantage over parent under 10 or more days' vegetative stage flooding [8,28].…”

Section: Introductionmentioning

confidence: 99%

Pyramiding Submergence Tolerance and Three Bacterial Blight Resistance Genes in Popular Rice Variety Hasanta through Marker-Assisted Backcross Breeding

et al. 2022

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Flash flooding (FF) along with bacterial blight (BB) outbreak are very destructive for rice production in the rainfed shallow-lowland (RSL) ecosystem. The presence of dynamic Xoo races with varying levels of genetic diversity and virulence renders their management extremely challenging under RSL. In this context, the marker-assisted improvement of plant resistance/tolerance has been proven as one of the most promising strategies towards the development of sustainable cultivars. The present study demonstrates the marker-assisted introgression of the submergence tolerant (Sub1) and three bacterial blight resistant genes (Xa21 + xa13 + xa5) into the genetic background of Hasanta, a long duration popular rice variety in the eastern coastal region of India. The rice genotypes, Swarna Sub1 (carrying Sub1) and IRBB66 (carrying Xa21 + xa13 + Xa7 + xa5 + Xa4) had maximum genetic similarity (0.84 and 0.73, respectively) with Hasanta; recurrent parent (RP) was used as donor. The forward analysis of target genes in F1s, IC1F1s and backcross (BC) generations was performed by linked/genic markers (Sub1bc2; pTA248, xa13prom and RM122), whereas background recovery of RP in each BC and segregating generations was performed utilizing 108 hypervariable SSR markers. Intervened speed breeding (SB) strategy and intensive phenotyping could lead the development of near isogenic lines (NILs) as to the RP in all basic traits. The performance of the near isogenic lines (NILs, BC2F3 and BC2F4), HS 232-411-391-756-37, HS 232-411-391-809-8, HS 232-411-391-756-18, HS 110-224-197-10-36, HS 232-411-391-809-81, HS 110-224-197-10-41 and HS 232-411-391-809-63 establishes the utility of marker-assisted backcross-breeding (MAB) and SB in accelerated trait introgression. The introgressed lines carrying Sub1 + Xa21 + xa13 + xa5 showed 76% to 91% survival under 14 days of submergence and durable BB resistance (percent disease index-PDI of 2.68 ± 0.26 to 6.22 ± 1.08 and lesion length, LL of 1.29 ± 0.12 to 4.2 ± 0.64 cm). Physiological analysis revealed that improved NILs, carrying Sub1 gene conquered adaptive physiological modulations, had reduced the consumption of soluble sugar and the degradation of total chlorophyll contents (TCC), and an enhanced level of Alcohol Dehydrogenase activity (ADH) and proline accumulation in all submergence regimes. The pyramided lines attained complete product profile of RP, that will contribute to sustainable rice production under RSL, particularly in the coastal region that has substantial acreage under the variety Hasanta.

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Introgression of dual abiotic stress tolerance QTLs (Saltol QTL and Sub1 gene) into Rice (Oryza sativa L.) variety Aiswarya through marker assisted backcross breeding

Cited by 24 publications

References 19 publications

Progress and prospects in harnessing wild relatives for genetic enhancement of salt tolerance in rice

Progress and prospects in harnessing wild relatives for genetic enhancement of salt tolerance in rice

Marker-Assisted Introgression of the Salinity Tolerance Locus Saltol in Temperate Japonica Rice

Pyramiding Submergence Tolerance and Three Bacterial Blight Resistance Genes in Popular Rice Variety Hasanta through Marker-Assisted Backcross Breeding

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