Introgression the &lt;i&gt;SalTol&lt;/i&gt; QTL into the Elite Rice Variety of Russia by Marker-Assisted Selection&lt;br&gt;

Усатов, А. В.; Алабушев, А. В.; Kostylev, P. I.; Azarin, Kirill; Makarenko, Maksim; Усатова, О. А.

doi:10.3844/ajabssp.2015.165.169

Cited by 22 publications

(16 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Further study on Saltol QTL assumed that the same HKT1;5 gene was responsible for salinity tolerance [17]. Following these results, Saltol QTLwas introgressed to local elite varieties in Asia, West Africa, and Russia through marker-assisted backcrossing [45–48]. In our RIL-QTL mapping, QTLs for high shoot K + concentration ( qK1 .…”

Section: Discussionmentioning

confidence: 99%

Identification and validation of QTLs for seedling salinity tolerance in introgression lines of a salt tolerant rice landrace ‘Pokkali’

2017

View full text Add to dashboard Cite

Salinity is a major threat to rice production worldwide. Several studies have been conducted to elucidate the molecular basis of salinity tolerance in rice. However, the genetic information such as quantitative trait loci (QTLs) and molecular markers, emanating from these studies, were rarely exploited for marker-assisted breeding. To better understand salinity tolerance and to validate previously reported QTLs at seedling stage, a set of introgression lines (ILs) of a salt tolerant donor line ‘Pokkali’ developed in a susceptible high yielding rice cultivar ‘Bengal’ background was evaluated for several morphological and physiological traits under salt stress. Both SSR and genotyping-by-sequencing (GBS) derived SNP markers were utilized to characterize the ILs and identify QTLs for traits related to salinity tolerance. A total of eighteen and thirty-two QTLs were detected using SSR and SNP markers, respectively. At least fourteen QTLs detected in the RIL population developed from the same cross were validated in IL population. Analysis of phenotypic responses, genomic composition, and QTLs present in the tolerant ILs suggested that the mechanisms of tolerance could be Na+ dilution in leaves, vacuolar Na+ compartmentation, and possibly synthesis of compatible solutes. Our results emphasize the use of salt injury score (SIS) QTLs in marker-assisted breeding to improve salinity tolerance. The tolerant lines identified in this study will serve as improved breeding materials for transferring salinity tolerance without the undesirable traits of Pokkali. Additionally, the lines will be useful for fine mapping and map-based cloning of genes responsible for salinity tolerance.

show abstract

Section: Discussionmentioning

confidence: 99%

Identification and validation of QTLs for seedling salinity tolerance in introgression lines of a salt tolerant rice landrace ‘Pokkali’

2017

View full text Add to dashboard Cite

show abstract

“…As is known, the adaptations plants to extreme environmental factors are manifested at different levels of organization of the organism (Negrao et al, 2011;Usatov et al, 2015). The study of morpho-physiological response to salinity is important with the standpoint of understanding the mechanisms of plant adaptation to stressful influences.…”

Section: Introductionmentioning

confidence: 99%

Effects of Salt Stress on Ion Balance at Vegetative Stage in Rice (<i>Oryza sativa</i> L.)

Azarin¹,

Алабушев²,

Усатов³

et al. 2016

OnLine Journal of Biological Sciences

View full text Add to dashboard Cite

Rice (Oryza sativa L.) plants were subjected to NaCl stress within 35 days. The shoot dry weight, root dry weight, total survival percentage and contents of inorganic ions in dry samples of stressed plants were measured to identify the physiological adaptive mechanisms by which rice plants at vegetative stage tolerate salt stresses. Laboratory testing of the 10 rice cultivars showed that the salt concentration for the treatment is especially significant for assessment of the relationship between ion accumulation and salt tolerance. Salt treatment strongly stimulated accumulations of Na + , Na + /K + ratio, Clin shoot and root and reduced K + , NO 3 contents in both organs under 1.2% NaCl stress condition, but not always under 0.6% NaCl concentration. It was shown significantly negative correlation (p<0.5) between the Na + content and dry weight of shoot and root under 1.2% NaCl stress condition. Also, was founded significantly negative impact (p<0.5) of Clions on biomass growth and survival rice plants and positive relationship (p<0.5) among the root dry weight and K + , NO 3 content under the high NaCl concentration (1.2% NaCl). So, in addition to Na + , the contributions of Clto abiotic stresses of rice should not be ignored.

show abstract

“…But selection on the provocative backgrounds of the needed recombinants is rather complicated. That is why it is necessary to brood the rice sorts with the high potential of the crop capacity and seeds quality using the modern instruments of biotechnology [13,14].…”

Section: Discussion Of the Results Of Researchmentioning

confidence: 99%

Assessment of Rice Sorts and Hybrids on the Salt Tolerance Using Laboratory Methods

Kostylev

Kudashkina

2016

EUREKA: Life Sciences

View full text Add to dashboard Cite

The article presents the results of the analysis of salt tolerance in rice samples by the laboratory methods at a concentration of NaCl – 1,5 %. The plants were grown in an incubator at temperature 26 °C. After 15 days, we measured the length of roots and shoots; the germination of seeds was counted. For the analysis of hybrids sensitive to salt Krasnodar varieties Novator with lines IR 52713-2B-8-2B-1-2, IR 74099-3R-3-3, NSIC Rc 106 – donor locus salt tolerance Saltol, using markers RM 493 and RM 7075. 90 plants were selected from the F2 plants which are analyzed by PCR, and the results of DNA analysis identified 6 of them as salt tolerant samples homozygous for the desired locus. From their offspring was selected the promising form to create salt-tolerant rice sorts.

show abstract

Introgression the <i>SalTol</i> QTL into the Elite Rice Variety of Russia by Marker-Assisted Selection<br>

Cited by 22 publications

References 22 publications

Identification and validation of QTLs for seedling salinity tolerance in introgression lines of a salt tolerant rice landrace ‘Pokkali’

Identification and validation of QTLs for seedling salinity tolerance in introgression lines of a salt tolerant rice landrace ‘Pokkali’

Effects of Salt Stress on Ion Balance at Vegetative Stage in Rice (<i>Oryza sativa</i> L.)

Assessment of Rice Sorts and Hybrids on the Salt Tolerance Using Laboratory Methods

Contact Info

Product

Resources

About