Inulin supplementation in rat model of pouchitis.

Abstract

Available data indicates potential effectiveness of prebiotic therapy in alleviating inflammation and prolonging the remission in inflammatory bowel disease. Documented successes of such therapies were the basis for this study. So far, there is no data related to the effectiveness of inulin application in symptomatic or severe pouchitis in humans or in animal model. The aim of the study was to determine the effect of inulin supplementation on the expression of intestinal inflammation and feeding efficiency in rats with induced pouchitis. Twenty-four Wistar rats were operated. After induction of pouchitis animals were randomly divided into control and supplementation groups receiving, respectively, semi-synthetic diet with or without inulin (in a lower (LD) or higher (HD) dose: 2.5 % or 5 % of total dietary content of mass) for a period of 6 weeks. Selected nutritional parameters were assessed throughout the study. Histopathological and immunohistochemical analysis of pouch mucosa specimens was also performed. The energy intake, weight gain, feeding efficiency, quality of stools were comparable in all studied groups. The intensity of inflammation (Moskovitz scale) and adaptive changes (Laumonier scale) did not differ between compared groups. The tissue expression of pro- and anti-inflammatory interleukins (IL-1α, IL-6, IL-10 and IL-12) was not different either. Inulin supplementation does not improve the quality of stools or the expression of intestinal inflammation in rats with induced pouchitis. It has no impact on the intensity of pouch adaptation or on feeding efficiency.

Type IV resistant starch increases cecum short chain fatty acids level in rats.

“…A large intake of dietary fiber improves serum lipid concentration, lowers blood pressure, improves blood glucose control in diabetes, aids in weight loss and appears to improve immune function. Furthermore, increased consumption of dietary fiber reduces risk of developing coronary heart disease, stroke, hypertension, diabetes, obesity and certain gastrointestinal diseases (Anderson et al, 2009, Dżymała-Czyż et al, 2011. Physiological effect of dietary fiber depends on its physicochemical properties mainly on solubility.…”

Cross Talk between Gut Microbiota and Immune System: From Pathophysiology to Potential Therapeutic Interventions in Inflammatory Bowel Disease

“…Prebiotics such as inulin, have been shown to induce the growth of SCFA-producing bacteria, including Lactobacillus, F. prausnitzii and Bifidobacterium [127]. Inulin has also been shown to improve histological lesions in patients with pouchitis [129]. FOS are known to increase the population of endogenous microflora, particularly Lactobacillus and Bifidobacterium [130].…”