Inulinase: An Important Microbial Enzyme in Food Industry

Mohan, Anand; Flora, Bableen; Girdhar, Madhuri

doi:10.1007/978-981-13-0053-0_12

Cited by 9 publications

(5 citation statements)

References 28 publications

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“…Family GH32 (Acidobacteria) comprises invertases and inulinases, enzymes that can be applied in food and fermentation processes [46,47]. GH17 (Proteobacteria) is composed of endoglucanases with activity against βglucan and laminarin, effective additives for the degradation of polysaccharides for animal feed [47]. Mutanases belonging to GH71 (Actinobacteria) family already showed activity against glucans present in dental plaque [48].…”

Section: Discussionmentioning

confidence: 99%

Cultivation-independent and cultivation-dependent metagenomes reveal genetic and enzymatic potential of microbial community involved in the degradation of a complex microbial polymer

et al. 2020

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Background: Cultivation-independent methods, including metagenomics, are tools for the exploration and discovery of biotechnological compounds produced by microbes in natural environments. Glycoside hydrolases (GHs) enzymes are extremely desired and important in the industry of production for goods and biofuel and removal of problematic biofilms and exopolysaccharide (EPS). Biofilms and EPS are complex, requiring a wide range of enzymes for a complete degradation. The aim of this study was to identify potential GH microbial producers and GH genes with biotechnological potential, using EPS-complex structure (WH15EPS) of Acidobacteria Granulicella sp. strain WH15 as an enrichment factor, in cultivation-independent and cultivation-dependent methods. We performed stable isotope probing (SIP) combined with metagenomics on topsoil litter amended with WH15EPS and coupled solid culture-EPS amended medium with metagenomics.

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Section: Discussionmentioning

confidence: 99%

Cultivation-independent and cultivation-dependent metagenomes reveal genetic and enzymatic potential of microbial community involved in the degradation of a complex microbial polymer

et al. 2020

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“…Figure 8: Shows the largest market size for FOS is in infant formula, the second is in food and beverages, the third is in dietary supplements, the fourth is in animal feeds and the smallest market size is in pharmaceutical [29].…”

Section: -2024 Fos Market Size ($ Million) By Applicationsmentioning

confidence: 99%

Technological Aspects of Fructo-Oligosaccharides (FOS), Production Processes, Physiological Properties, Applications and Health Benefits

Ibrahim¹

2021

J Food Chem Nanotechnol

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Fructo-oligosaccharides (FOS) has two other names oligofructose and oligofructan are naturally presents in fruits and vegetables but are mainly produced enzymatically from sucrose or inulin as enzymes substrates. Its chemical structures are short chain of fructose polymer with terminal glucose unit comparing to inulin that has similar chemical structure but at higher degree of fructose polymerization (DP). Inulin is present at high level in Chicory roots and Jerusalem artichoke. The enzyme fructosyl-transferase or the enzyme ß-fructo-furanosidase are used for the production of FOS from sucrose as substrate, while the enzyme endoinulinase is used for the production of FOS from inulin as a substrate. Fructooligosaccharides (FOS) are used in food applications, dietary supplements and nutrition bars as low-calorie sweetener for diabetes in replacement to sucrose, also used in pharmaceutical drugs and cosmetics products formulation. FOS for health benefits is used in dietary fiber and as prebiotics for constipation and to enhance the growth of beneficial bacteria in the colon respectively. Other health benefits for FOS are increase mineral absorption in digesting system, and decrease, serum cholesterol/triglycerides. FOS are General Recognized as Safe (GRAS) in United States, and also approved for use by other international regulatory agencies in food products, infant formulas, pharmaceutical and in other applications.

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“…Inulinase is obtained from microbial sources such as bacteria, fungi, and yeast by enzymatic management (Rawat et al, 2021). The inulinase acts as a catalyst in the hydrolysis of inulin which will form fructose as an important nutrient in a variety of foods (Mohan et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Processing of Red Dahlia Tubers in Produce Inulin Extract and Material Proximate Testing

Sunarti¹,

Ginting

2022

nuscientech

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Dahlia tubers from Berastagi, North Sumatra, are plants that contain carbohydrates and contain high levels of inulin. Inulin is very good as a dietary fiber and has other physiological functions such as lowering blood sugar and blood fat, anticancer, regulating intestinal microbial flora, increasing absorption of minerals and vitamins. Currently, the utilization of dahlia tubers is not optimal in the community and is considered as agricultural waste, therefore it is necessary to manage dahlia tubers in producing inulin extract and study the proximate material, considering that previous studies still obtained varying results. This study aimed to obtain inulin extract and its yield value and to measure the proximate material of red dahlia tuber. The extraction method used is based on the solubility of inulin in water at a temperature of 800 C. And precipitation are carried out with 70% ethanol. Proximate examination of the material consisted of water content using the heating method, ash content using the gravimetric method, fat and crude fiber content using the Soxhlet method, determination of protein content using the Macro Kjedhal method, and carbohydrate content using the proximate method using the carbohydrate percentage formula. The results obtained were 48,25% Inulin Flour yield, the proximate results obtained 80,8% water content, 0,36% ash content, 0,33% total fat content, 1,29% crude fiber content, protein content 1,15%, Carbohydrate content 14.6%. From this study, it can be concluded that dahlia tubers contain high carbohydrates and low-fat content, have crude fiber and protein that can be used as low-calorie foodstuffs.

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Inulinase: An Important Microbial Enzyme in Food Industry

Cited by 9 publications

References 28 publications

Cultivation-independent and cultivation-dependent metagenomes reveal genetic and enzymatic potential of microbial community involved in the degradation of a complex microbial polymer

Cultivation-independent and cultivation-dependent metagenomes reveal genetic and enzymatic potential of microbial community involved in the degradation of a complex microbial polymer

Technological Aspects of Fructo-Oligosaccharides (FOS), Production Processes, Physiological Properties, Applications and Health Benefits

Processing of Red Dahlia Tubers in Produce Inulin Extract and Material Proximate Testing

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