Invasion front‐specific expression and prognostic significance of microRNA in colorectal liver metastases

Kahlert, Christoph; Klupp, Fee; Brand, Karsten; Lasitschka, Felix; Diederichs, Sven; Kirchberg, Johanna; Rahbari, Nuh N.; Dutta, Shamik; Bork, Ulrich; Fritzmann, Johannes; Reißfelder, Christoph; Koch, Moritz; Weitz, Juergen

doi:10.1111/j.1349-7006.2011.02023.x

Cited by 75 publications

(60 citation statements)

References 54 publications

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“…Previous studies demonstrated that miR-19b overexpression was related to 5-FU resistance in colon cancer cells (Kurokawa et al 2012) and was an adverse prognostic marker of recurrence and overall survival in patients with colorectal cancer (Kahlert et al 2011). Aberrant expression of miR-146a was correlated with complete remission rate, higher overall response rate and longer progression-free survival time in patients with diffuse large B-cell lymphoma (Zhong et al 2012).…”

Section: Discussionmentioning

confidence: 89%

Serum Levels of miR-19b and miR-146a as Prognostic Biomarkers for Non-Small Cell Lung Cancer

Cao

et al. 2014

Tohoku J. Exp. Med.

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MicroRNA (miRNA) is a type of small non-coding RNA molecule that has important roles in cancer initiation, promotion and progression by negatively regulating gene expression. In this study, we explored the role of miRNAs in the prognosis of patients with non-small cell lung cancer (NSCLC). The miRNA expression profiles were determined in 5 pairs of NSCLC and paracancerous tissues (3 adenocarcinomas and 2 squamous cell carcinomas). Aberrantly expressed miRNAs were validated by quantitative real-time PCR (qRT-PCR) in 61 pairs of NSCLC and paracancerous tissues. Differentially expressed miRNAs were further analyzed in sera from 94 healthy subjects and 94 advanced NSCLC patients receiving platinumbased chemotherapy. Three miRNAs (miR-19b, miR-146a, and miR-223) were significantly dysregulated in NSCLC tissues (P < 0.05). High miR-19b and low miR-146a expression in NSCLC tissues were associated with higher TNM stage, lymph node metastasis and poorer survival (P < 0.05). The serum levels of miR-19b in NSCLC patients were significantly higher (P < 0.001), whereas serum levels of miR-146a were significantly lower (P < 0.001), compared with those in controls. Serum levels of miR-19b and miR-146a were associated with overall survival of NSCLC patients (P < 0.05). Patients with low serum level of miR-19b and high serum level of miR-146a achieved a higher overall response rate and longer survival time (P < 0.05). These data suggest that miR-19b and miR-146a are potential biomarkers for the prediction of survival and response to chemotherapy in NSCLC.

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Section: Discussionmentioning

confidence: 89%

Serum Levels of miR-19b and miR-146a as Prognostic Biomarkers for Non-Small Cell Lung Cancer

Cao

et al. 2014

Tohoku J. Exp. Med.

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“…The miR17-92 cluster has been designated oncomir-1 (31), and can promote proliferation and angiogenesis, inhibit differentiation, and sustain cell survival (29,30). Elevated miR17-92 levels have been associated with invasion and metastasis of colorectal cancer cells (32), and poorer survival (33). miR19a and miR19b in particular are key oncogenic determinants (29,30), and both were significantly elevated with the HRM diet compared with the entry diet.…”

Section: Discussionmentioning

confidence: 99%

Dietary Manipulation of Oncogenic MicroRNA Expression in Human Rectal Mucosa: A Randomized Trial

Humphreys

Conlon

Young

et al. 2014

Cancer Prevention Research

100

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High red meat (HRM) intake is associated with increased colorectal cancer risk, while resistant starch is probably protective. Resistant starch fermentation produces butyrate, which can alter microRNA (miRNA) levels in colorectal cancer cells in vitro; effects of red meat and resistant starch on miRNA expression in vivo were unknown. This study examined whether a HRM diet altered miRNA expression in rectal mucosa tissue of healthy volunteers, and if supplementation with butyrylated resistant starch (HRMþHAMSB) modified this response. In a randomized cross-over design, 23 volunteers undertook four 4-week dietary interventions; an HRM diet (300 g/day lean red meat) and an HRMþHAMSB diet (HRM with 40 g/day butyrylated high amylose maize starch), preceded by an entry diet and separated by a washout. Fecal butyrate increased with the HRMþHAMSB diet. Levels of oncogenic mature miRNAs, including miR17-92 cluster miRNAs and miR21, increased in the rectal mucosa with the HRM diet, whereas the HRMþHAMSB diet restored miR17-92 miRNAs, but not miR21, to baseline levels. Elevated miR17-92 and miR21 in the HRM diet corresponded with increased cell proliferation, and a decrease in miR17-92 target gene transcript levels, including CDKN1A. The oncogenic miR17-92 cluster is differentially regulated by dietary factors that increase or decrease risk for colorectal cancer, and this may explain, at least in part, the respective risk profiles of HRM and resistant starch. These findings support increased resistant starch consumption as a means of reducing risk associated with an HRM diet. Cancer Prev Res; 7(8); 786-95. Ó2014 AACR.

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“…6) Therefore, let-7b may play an important role in CRC metastasis. The intracellular expression levels of let-7c and let-7g have been associated with the metastasis of colorectal and breast cancers, respectively.…”

Section: Discussionmentioning

confidence: 99%

Effects of Zebularine on Invasion Activity and Intracellular Expression Level of let-7b in Colorectal Cancer Cells

Tanaka

Hosokawa

Matsumura

et al. 2017

Biological & Pharmaceutical Bulletin

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The effects of zebularine, a DNA methyltransferase inhibitor, on the invasion activity as well as intracellular expression level of let-7b, tumor suppressor microRNA, were examined in three human colorectal cancer (CRC) cell lines: SW480, SW620, and oxaliplatin-resistant SW620 (SW620/OxR). Zebularine suppressed the invasion activity of SW620 and SW620/OxR cells. The intracellular expression level of let-7b was upregulated by zebularine in SW620 and SW620/OxR cells. The overexpression of let-7b by the transfection of let-7b mimic suppressed invasion activity in SW620 and SW620/OxR cells. These results suggest that zebularine may inhibit invasion activity by up-regulating the intracellular expression level of let-7b in high-invasive CRC cells.Key words invasion; zebularine; let-7b; oxaliplatin-resistant; colorectal cancer DNA methylation is a major epigenetic mechanism of gene expression level. Zebularine (Zeb) has been shown to inhibit DNA methyltransferase (DNMT). Furthermore, Zeb and other DNMT inhibitors, such as decitabine (DAC) and azacitidine (AC), exert differential effects on gene expression levels.1) Flotho et al. reported that Zeb did not regulate the expression levels of a large number of mRNAs or markers for genome-wide methylation.1) We previously showed that DAC suppressed invasion activity with the up-regulation of epithelial cell marker expression levels not only in parental SW620 cells, high-invasive colorectal cancer (CRC) cells, but also in oxaliplatin (L-OHP)-resistant SW620 (SW620/OxR) cells. 2)Pakneshan et al. demonstrated that AC enhanced invasion activity by up-regulating the urokinase plasminogen activator in prostate cancer.3) However, information on the effects of Zeb on invasion activity is limited.MicroRNAs (miRNAs), small non-coding RNAs that suppress target mRNA, play important roles in cancer metastasis. Members of the let-7 family (let-7a, let-7b, let-7c, let-7d, let-7e, let-7f, let-7g, and let-7i in addition to miR-98 and miR-202) are tumor suppressor miRNAs. 4) Kang et al. previously reported that let-7b suppressed invasion activity in gastric cancer.5) In addition, Kahlert et al. showed that let-7b expression level in CRC tumor invasion front was lower than that in CRC tumor center.6) The expression levels of let-7 family members are also regulated by DNA methylation. The location of let-7b is in the 3′-untranslated region of MIRLET7BHG as a long intergenic noncoding RNA. 7) Nishi et al. found that intracellular expression level of let-7b may be regulated by the DNA methylation status of CpG islands near transcription start site (TSS) of MIRLET7BHG.7) Kobayashi et al. showed that let-7 family members were more abundant in exosomes, small membrane vesicles of approximately 100 nm in diameter, from high-invasive ovarian cancer cells than in those from low-invasive ovarian cancer cells.8) In addition, Wong et al. reported that the expression levels of let-7 family members were upregulated by Zeb in nasopharyngeal carcinoma cells. We previously demonstrated that the invasion activ...

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Invasion front‐specific expression and prognostic significance of microRNA in colorectal liver metastases

Cited by 75 publications

References 54 publications

Serum Levels of miR-19b and miR-146a as Prognostic Biomarkers for Non-Small Cell Lung Cancer

Serum Levels of miR-19b and miR-146a as Prognostic Biomarkers for Non-Small Cell Lung Cancer

Dietary Manipulation of Oncogenic MicroRNA Expression in Human Rectal Mucosa: A Randomized Trial

Effects of Zebularine on Invasion Activity and Intracellular Expression Level of let-7b in Colorectal Cancer Cells

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