Invasion inhibition by a MEK inhibitor correlates with the actin-based cytoskeleton in lung cancer A549 cells

Niu, Junmei; Mo, Qingjiang; Wang, Helin; Li, Mingying; Cui, Junwei; Li, Zhenyun; Li, Zhen-kui

doi:10.1016/j.bbrc.2012.04.109

Cited by 10 publications

(8 citation statements)

References 17 publications

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“…Many cytoskeleton-associated proteins of lung cancer cells have been intensively investigated (Bernal et al, 1983;Chen et al, 2010Chen et al, , 2011Chang et al, 2012;Niu et al, 2012). SCIN (scinderin) is a calcium-dependent actin filament serving and capping protein (Marcu et al, 1998).…”

Section: Introductionmentioning

confidence: 98%

Lentivirus-mediated silencing of SCIN inhibits proliferation of human lung carcinoma cells

Liu

Shi

Liu

et al. 2015

Gene

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Section: Introductionmentioning

confidence: 98%

Lentivirus-mediated silencing of SCIN inhibits proliferation of human lung carcinoma cells

Liu

Shi

Liu

et al. 2015

Gene

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“…Dynamic changes in the cytoskeleton, such as cell migration and differentiation, are the basis of cell function (Janmey, 1998;Niu et al, 2012). The cytoskeleton has been increasingly recognized as the central regulator of OC function and differentiation (Jurdic et al, 2006;Vaananen and Laitala-Leinonen, 2008).…”

Section: Introductionmentioning

confidence: 99%

New roles of filopodia and podosomes in the differentiation and fusion process of osteoclasts

et al. 2014

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ABSTRACT. The cytoskeleton mediates various cellular processes such as differentiation and fusion, including in the filopodia and podosomes. However, apart from cell migration and formation of the sealing zone, little is known regarding the changes and related regulatory mechanisms of the cytoskeleton and additional roles of the filopodia and podosomes during the differentiation and fusion of osteoclasts. The cytomorphology and cytoskeleton of osteoclasts in the differentiation process were evaluated using tartrate-resistant acid phosphatase staining and immunofluorescence staining. Moreover, the expression levels of Rho GTPases and enzymes related to osteoclast differentiation and bone resorption were detected by quantitative reverse transcription-polymerase chain reaction. We detected 3 types of filopodia in osteoclast precursors and only 1 type of filopodia in undifferentiated cells. Mature osteoclasts were completely devoid of filopodia. Interestingly, cell fusion was highly specific, and the fusion New roles of filopodia and podosomes of osteoclasts initially occurred to the filopodia. Confocal images revealed that F-actin and microtubules significantly differed among fused cells. These results suggest that filopodia and podosomes not only play important roles in cell migration and the formation of sealing zones but also in the prefusion selectivity of 2 cells and the movement direction of the cell nucleus and cytoplasm during the fusion process. In addition, cdc42v1, RhoU, and RhoF regulate the formation of 3 types of filopodia during various stages of differentiation, while Rac1, Rac2, and filament A may be associated with cell selectivity during the fusion process.

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“…Cytoskeleton plays essential roles in the mitosis, migration and invasion of carcinoma cells (5). Many cytoskeletonassociated proteins of prostate cancer cells have been intensively investigated (6)(7)(8)(9)(10). SCIN is a calcium dependent actin filament serving and capping protein (11).…”

Section: Introductionmentioning

confidence: 99%

Suppression of SCIN inhibits human prostate cancer cell proliferation and induces G0/G1 phase arrest

Wang

Sun

et al. 2013

International Journal of Oncology

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DNA polymerase kappa (POLkappa) is a low fidelity translesional DNA polymerase implicated in spontaneous and DNA damage-induced mutagenesis. We have previously shown that POLkappa was frequently overexpressed in human lung cancer tissues as compared with their matched non-tumorous tissue counterpart. In the present study, we found a close correlation between elevated POLkappa expression and p53 inactivation in lung cancer tissues. To investigate whether POLK expression might be regulated by p53, we have determined the transcriptional initiation site of POLK gene and examined its promoter activity in A549, H358-129, and PC-3 human lung cancer cell lines. Wild-type p53, but not a mutant p53 (R273H) devoid of the DNA-binding activity, strongly inhibited POLK promoter activity in these cells. In addition, POLK promoter exhibited a significantly higher activity in p53-/- murine embryo fibroblasts (MEF) than in p53+/- and p53+/+ MEF. These results link p53 status with POLkappa expression and suggest that loss of p53 function may in part contribute to the observed POLkappa upregulation in human lung cancers.

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Invasion inhibition by a MEK inhibitor correlates with the actin-based cytoskeleton in lung cancer A549 cells

Cited by 10 publications

References 17 publications

Lentivirus-mediated silencing of SCIN inhibits proliferation of human lung carcinoma cells

Lentivirus-mediated silencing of SCIN inhibits proliferation of human lung carcinoma cells

New roles of filopodia and podosomes in the differentiation and fusion process of osteoclasts

Suppression of SCIN inhibits human prostate cancer cell proliferation and induces G0/G1 phase arrest

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