Lentivirus-mediated silencing of SCIN inhibits proliferation of human lung carcinoma cells

Liu, Hongxu; Shi, Daiwang; Liu, Tieqin; Yu, Zehao; Zhou, Chuanjiang

doi:10.1016/j.gene.2014.10.013

Cited by 17 publications

(15 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consistent with this conclusion, SCIN-knockdown was revealed to inhibit breast carcinoma cell proliferation in vitro in the present study. This observation was in accordance with studies by Liu et al (10) and Wang et al (9), who revealed that SCIN overexpression enhanced human lung and prostate carcinoma cell proliferation. Therefore, as SCIN governs filamentous actin (F-actin) cytoskeleton remodeling, it is possible that SCIN's effects on cell proliferation may be mediated through F-actin.…”

Section: Scin Expression --------------------------------------------supporting

confidence: 93%

“…Earlier studies indicated that SCIN serves an important role in the regulation of various cellular processes, including proliferation, migration and differentiation. In human prostate cancer and lung carcinoma cells, SCIN suppression inhibited cell proliferation (9,10). Conversely, in megakaryoblastic leukemia, SCIN overexpression inhibited cell proliferation and tumorigenesis via activating the ras-related C3 botulinum toxin substrate/p21-activated kinase/mammalian mitogen-activated protein kinase kinase, mitogen-activated protein kinase kinase 4/c-jun n-terminal kinase/c-jun, and rapidly accelerated fibrosarcoma kinase/mitogen-activated protein kinase kinase/extracellular signal-regulated kinase signaling pathways (11).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Scinderin‑knockdown inhibits proliferation and promotes apoptosis in human breast carcinoma cells

Jian

Zhang

Wang³

et al. 2018

Oncol Lett

View full text Add to dashboard Cite

Previous studies have reported that scinderin (SCIN) affects multiple cellular processes, including proliferation, migration and differentiation in cancer. However, the specific role of SCIN in breast cancer (BC) cells is unknown. Immunohistochemistry was used to investigate SCIN expression in 46 BC and 21 mammary fibroadenoma or fibroadenomatoid hyperplasia tissue samples. SCIN expression was ablated in MDA-MB-231 and T-47D cells using lentivirus-mediated small interfering RNA technology. Cell proliferation was tested using Celigo and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assays. Cell apoptosis was analyzed by measuring Caspase 3/7 activity and annexin-V staining. The results of the present study demonstrated that SCIN expression was elevated in BC tissues compared with mammary fibroadenoma or fibroadenomatoid hyperplasia tissues. Specifically, higher SCIN expression was observed in Ki-67-positive BC tissues (78.6%) compared with Ki-67-negative BC tissues. Furthermore, knockdown of SCIN expression in the BC cell lines significantly suppressed cell proliferation and induced apoptosis. The data presented in the present study indicate that SCIN serves an important role in the development of breast cancer.

show abstract

Section: Scin Expression --------------------------------------------supporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Scinderin‑knockdown inhibits proliferation and promotes apoptosis in human breast carcinoma cells

Jian

Zhang

Wang³

et al. 2018

Oncol Lett

View full text Add to dashboard Cite

show abstract

“…prostate cancer cell line, and suppression of SCIN results in decreased cell proliferation and G0/G1 phase arrest [16]. High SCIN expression has also been observed in human lung cancer specimens, and the level of SCIN is associated with cell proliferation in human lung carcinoma cell lines [17]. A quantitative proteomic analysis showed that SCIN was a cisplatin-resistant marker in the HT1376 human bladder cancer cell line [18], and SCIN knockdown effectively reduces cell proliferation and migration in the SGC7901 human GC cell line [19].…”

Section: Scin Is Highly Expressed In Both Human Prostate Cancer Specimentioning

confidence: 99%

Scinderin promotes the invasion and metastasis of gastric cancer cells and predicts the outcome of patients

Liu

Chen

et al. 2016

Cancer Letters

View full text Add to dashboard Cite

Invasion and metastasis are major malignant characteristics of human gastric cancer (GC), but the underlying molecular mechanisms are poorly understood. Recent studies have shown that scinderin (SCIN), an actin severing and capping protein that regulates the actin cytoskeleton, is involved in the proliferation and migration of certain cancer cells. Accordingly, this study aimed to investigate the potential role of SCIN in the invasion and metastasis of human GC cells and to evaluate its prognostic value for GC patients. We found that high levels of SCIN expression in GC tumors were correlated with poor overall survival of patients. Silencing of SCIN effectively suppressed the migratory and invasive capabilities of human GC cells in vitro and tumorigenicity and metastasis in vivo. Furthermore, knockdown of SCIN markedly inhibited the formation of filopodia, decreasing GC cell migration and the expression of Cdc42, an important regulator of filopodia by GC cells. These findings suggest that SCIN may be a novel prognostic marker and a potential therapeutic target in human GC.

show abstract

“…Suppression of SCIN inhibits prostate cancer cell proliferation and induces G0/G1 phase arrest. High SCIN expression has also been observed in human lung cancer specimens and cell lines . However, SCIN expression was negative in megakaryoblastic leukemia cells .…”

Section: Introductionmentioning

confidence: 94%

Decreased SCIN expression, associated with promoter methylation, is a valuable predictor for prognosis in acute myeloid leukemia

Zhang

Zhou

et al. 2018

Molecular Carcinogenesis

View full text Add to dashboard Cite

The present study was aimed to investigate SCIN expression as well as promoter methylation and further explore their clinical relevance in acute myeloid leukemia (AML) patients. Real-time quantitative PCR was carried out to detect the expression level of SCIN in 119 AML patients and 37 healthy controls. Real-time quantitative methylation-specific PCR and bisulfite sequencing PCR were carried out to detect SCIN promoter methylation levels in 103 AML patients and 29 controls. As compared with controls, the level of SCIN transcript was significantly down-regulated in AML patients (P = 0.001), and the level of methylated SCIN promoter was significantly higher in AML patients (P = 0.001). Moreover, the level of promoter methylation was weakly negatively correlated with SCIN expression in AML patients (R = -0.265, P = 0.027). Demethylation of SCIN promoter by 5-aza-2'-deoxycytidine could restore its expression in leukemic cell line THP1. The age of SCIN patients was significantly higher and C/EBPA mutation was significantly less than SCIN patients (P = 0.039 and 0.038, respectively). Moreover, the rate of complete remission (CR) of SCIN patients was significantly lower than SCIN patients (P = 0.009). Kaplan-Meier analysis showed that low SCIN expression was associated with shorter overall survival (P = 0.036). Cox regression analysis demonstrated low SCIN expression was an independent poor prognostic factor (P = 0.047). Furthermore, SCIN expression was restored in those patients who achieved CR after induction therapy (P = 0.003). These findings indicate that decreased SCIN expression associated with its promoter methylation is a valuable biomarker for predicting adverse prognosis in AML patients.

show abstract

Lentivirus-mediated silencing of SCIN inhibits proliferation of human lung carcinoma cells

Cited by 17 publications

References 21 publications

Scinderin‑knockdown inhibits proliferation and promotes apoptosis in human breast carcinoma cells

Scinderin‑knockdown inhibits proliferation and promotes apoptosis in human breast carcinoma cells

Scinderin promotes the invasion and metastasis of gastric cancer cells and predicts the outcome of patients

Decreased SCIN expression, associated with promoter methylation, is a valuable predictor for prognosis in acute myeloid leukemia

Contact Info

Product

Resources

About