Invasion of Malignant Cells into &lt;sup&gt;51&lt;/sup&gt;Cr-Labeled Host Tissues in Organotypical Culture

Mareel, Marcus; Bruyne, Georges De; Ridder, Leo De

doi:10.1159/000225171

Cited by 15 publications

(3 citation statements)

References 5 publications

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“…An attempt to apply the 51Cr-release assay, widely Used in immunocytoxicity tests, to models of invasion in organ culture failed (69). Similar amounts of radioactivity were found in the medium of both controls and invaded tissues, quite in contrast with what was expected from the histology of parallel fixed cultures.…”

Section: Release Assaysmentioning

confidence: 97%

“…Release assays were designed to measure the breakdown products which resulted from the lytic activities of invading cells (36,47,56,57,64,(66)(67)(68)(69)(70)(71)(72)(73). Most authors believe that invading cells release enzymes that exert a lyric effect on the normal tissue (for recent discussion, see Proteinases and Invasion, edited by P. Str~iuli, A.J.…”

Section: Release Assaysmentioning

confidence: 99%

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Invasion in vitro

Mareel

1983

Cancer Metast Rev

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To understand the mechanisms of tumour invasion a number of in vitro assays have been developed, in which test cells (invasive or non-invasive) have been confronted with normal cells or tissues (the host) and cultured in various ways. We will discuss here the methods by which invasion can be judged in such assays. Histology is the method that is used successfully by pathologists for the evaluation of tumour invasiveness in vivo. The major difficulty is that the relationship between the non-invasive counterpart and the host tissue is not known in vitro or in vivo. Some authors have attempted to quantify invasion on histologic sections, but a generally accepted method is not yet available. The potential contribution of time lapse cinematography to the study of the kinetic aspects of invasion is obvious. Optical systems which overcome the relative lack of transparency of three-dimensional tissues, however, do not exist. Electron microscopy has contributed by providing details about the interactions between invasive cells on the one hand and normal cells as well as intercellular matrices on the other hand. Subpopulations of highly invasive and metastatic cells were collected after repeated migration through normal tissues in two-compartment assays. Attempts to evaluate the cytotoxic effect of invasive cells confronting radiolabelled cells or tissues have produced equivocal results. In contrast, measurements of radioactivity in the medium of labelled substrata have demonstrated the lytic effect of invasive cells on the extracellular matrix. It is concluded that further search for better methods to analyze and quantitate invasion is needed. So far, corroborative analysis by various methods seems to be the best policy.

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Section: Release Assaysmentioning

confidence: 97%

Section: Release Assaysmentioning

confidence: 99%

Invasion in vitro

Mareel

1983

Cancer Metast Rev

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“…Attempts to quantitate the destruction of PHF and mesonephros invaded by HeLa cells by 5~Cr-release were not successful (185), but observations have been published (186,187), showing that phagocytosis by invading cells may at least partly be held responsible for the disappearance of the host tissue. From these as well as from numerous histopathological findings one can hardly draw any other conclusion than the traditional one, according to which invasion and tissue destruction are closely related.…”

Section: Enzymatic Factors Involved In Invasionmentioning

confidence: 99%

Invasiveness of transformed bladder epithelial cells

Kieler

1984

Cancer Metast Rev

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The invasiveness of bladder tumors has been studied in man, experimental animals, and in tissue culture by numerous authors. The prognostic importance of cellular markers for invasiveness is stressed, and the usefulness of histopathological and cytologic grading, cytogenetic studies, antigenic investigations, and enzymatic characterization is discussed. The invasiveness of bladder cells has frequently been examined in transplantation and explantation experiments. In human urothelial cell cultures three grades of transformation are defined, and a correlation has been established between the invasiveness of these cell lines in a three-dimensional in vitro model and their tumorigenicity in nude mice. The mechanism of tumor invasion is discussed, and it is recommended in future research to make a distinction between invasion en bloc and cellular infiltration.

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Development of a quantitative method to analyse tumour cell invasion in organotypic culture

et al. 2005

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Tumour invasion is a dynamic process occurring in three dimensions and involving interactions between both tumour and stromal cells. Experimental analysis of squamous carcinoma cell invasion has often used the organotypic gel culture system, which is generated by plating tumour cells on to a synthetic stroma composed of a collagen gel embedded with fibroblasts. Unfortunately, quantitation of invasion in these organotypic gels has relied largely on subjective pathological opinion, which may be influenced by different patterns of tumour cell infiltration. Therefore a computer-assisted digital image analysis system that assesses invasion objectively and provides a numerical 'Invasion Index' was developed. The Invasion Index, by combining depth and pattern of invasion in a single value, establishes a quantitative value that allows assessment of the influences of positive and negative regulation of tumour invasion. These data demonstrate that the organotypic gel system is a robust, accurate, and reproducible method for measuring tumour cell invasion. They also show that the Invasion Index can be used after organotypic gels have been implanted in mice for up to 6 weeks. Illustrative examples of how various factors influence the invasion of squamous carcinoma cells in three dimensions both in vitro and in vivo are provided.

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Invasion of Malignant Cells into <sup>51</sup>Cr-Labeled Host Tissues in Organotypical Culture

Cited by 15 publications

References 5 publications

Invasion in vitro

Invasion in vitro

Invasiveness of transformed bladder epithelial cells

Development of a quantitative method to analyse tumour cell invasion in organotypic culture

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