Inversion of layer‐specific cadherin expression profiles and maintenance of cytoarchitectonic areas in the allocortex of the reeler mutant mouse

Stoya, Gudrun; Redies, Christoph; Schmid-Hertel, Nicole

doi:10.1002/cne.23572

Cited by 10 publications

(11 citation statements)

References 62 publications

(83 reference statements)

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“…In the present work, we study the expression profiles of Pcdh19 and Ncdh in the limbic structures in postnatal brain sections. Our data corroborate previous studies on the expression of Pcdh19 (Gaitan & Bouchard, ; Kim, Chung, Sun, & Kim, ; Dibbens et al, ; Hertel et al, ; Stoya, Redies, & Schmid‐Hertel, ) and Ncdh (Redies & Takeichi, ; Obst‐Pernberg et al, ). We extend these studies by adding more detail and provide an extensive overview of expression of both cadherins in the limbic structures, such as the amygdala, the hippocampus and the ventral hypothalamus.…”

Section: Discussionsupporting

confidence: 92%

Expression profile of N‐cadherin and protocadherin‐19 in postnatal mouse limbic structures

Schaarschuch

Hertel

2017

J of Comparative Neurology

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Cadherins are a superfamily of calcium-dependent cell adhesion molecules that are involved in brain development and organization. Previous genetic studies revealed that mutations in protocadherin-19 (Pcdh19) lead to an epilepsy syndrome with a variable degree of cognitive disability. Seizure origins are located in the frontotemporal and limbic structures. Expression studies of Pcdh19 in mouse confirmed a widespread presence during brain development while the function and the pathogenesis of Pcdh19 are still unknown in mammals. The neuronal cadherin (N-cadherin; Ncdh) is known for its important role in neurulation, brain development and regulation of synaptic function. Studies in zebrafish revealed that both cadherins can interact with each other in cell adhesion. We investigated the expression pattern of Pcdh19 and Ncdh in limbic structures at four postnatal stages of C57BL/6J mice by using double-label in situ hybridization. Results confirm a strong expression of both, Ncdh and Pcdh19, in structures of the limbic system with overlapping expression patterns particularly within regions of the amygdala, the hippocampus and the ventral hypothalamus. A detailed analysis of the limbic system highlight clear expression boundaries between several nuclei and reveal the fine regulation of Pcdh19 and Ncdh expression during the first postnatal week. Most expression patterns of both cadherins remain constant with a few exceptions particularly between P2 and P5.

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Section: Discussionsupporting

confidence: 92%

Expression profile of N‐cadherin and protocadherin‐19 in postnatal mouse limbic structures

Schaarschuch

Hertel

2017

J of Comparative Neurology

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“…7 ). Recent work using double ISH labeling validates the layer-specific expression patterns we find for cadherins in the MEC [ 23 ], while other well-characterized genes such as reelin and calbindin [ 22 , 75 ] also have expected expression patterns. Our analysis identifies a further 767 genes with layer-specific or enriched expression and 799 genes with dorsoventral expression.…”

Section: Discussionsupporting

confidence: 62%

“…Our understanding of the architecture and functions of MEC may benefit from similar approaches. While detailed anatomical and histochemical studies have shown that certain genes, including reelin, calbindin [ 22 ] and some cadherins [ 23 ], identify cell populations associated with particular layers of the MEC, we know very little about the identity, laminar or dorsoventral organization of the vast majority of genes expressed in the MEC. This is a difficult problem to address for the MEC as its borders with adjacent structures are ambiguous, it has a dorsoventral as well as a laminar organization and its similarity to other cortical structures is unclear.…”

Section: Introductionmentioning

confidence: 99%

Laminar and Dorsoventral Molecular Organization of the Medial Entorhinal Cortex Revealed by Large-scale Anatomical Analysis of Gene Expression

et al. 2015

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Neural circuits in the medial entorhinal cortex (MEC) encode an animal’s position and orientation in space. Within the MEC spatial representations, including grid and directional firing fields, have a laminar and dorsoventral organization that corresponds to a similar topography of neuronal connectivity and cellular properties. Yet, in part due to the challenges of integrating anatomical data at the resolution of cortical layers and borders, we know little about the molecular components underlying this organization. To address this we develop a new computational pipeline for high-throughput analysis and comparison of in situ hybridization (ISH) images at laminar resolution. We apply this pipeline to ISH data for over 16,000 genes in the Allen Brain Atlas and validate our analysis with RNA sequencing of MEC tissue from adult mice. We find that differential gene expression delineates the borders of the MEC with neighboring brain structures and reveals its laminar and dorsoventral organization. We propose a new molecular basis for distinguishing the deep layers of the MEC and show that their similarity to corresponding layers of neocortex is greater than that of superficial layers. Our analysis identifies ion channel-, cell adhesion- and synapse-related genes as candidates for functional differentiation of MEC layers and for encoding of spatial information at different scales along the dorsoventral axis of the MEC. We also reveal laminar organization of genes related to disease pathology and suggest that a high metabolic demand predisposes layer II to neurodegenerative pathology. In principle, our computational pipeline can be applied to high-throughput analysis of many forms of neuroanatomical data. Our results support the hypothesis that differences in gene expression contribute to functional specialization of superficial layers of the MEC and dorsoventral organization of the scale of spatial representations.

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“…There are numerous reports on expression of these cadherins in embryonic vertebrate CNS (Wohrn et al, 1998, 1999; Redies et al, 2001; Becker and Redies, 2003; Luo et al, 2004; Gaitan and Bouchard, 2006; Liu et al, 2006; Emond et al, 2009; Tai et al, 2010; Liu et al, 2010; Terakawa et al, 2013), but only a few on their distribution in the brain of adult vertebrates (Dean et al, 2007; Kim et al, 2007; Hertel et al, 2008, 2012; Hertel and Redies, 2011; Stoya et al, 2014). Moreover, studies on their expression in the adult brains focus mainly on a subset of brain structures (e.g.…”

Section: Discussionmentioning

confidence: 99%

“…In developing and adult mice and rats, pcdh17 and pcdh19 are detected in most cortical regions, basal ganglia, amygdale and/or hippocampus (Kim et al, 2007; Hertel et al, 2008; Hertel and Redies, 2011; Stoya et al, 2014). Both pcdhs are found in zebrafish dorsal telencephalon (pallium).…”

Section: Discussionmentioning

confidence: 99%

Differential expression of protocadherin‐19, protocadherin‐17, and cadherin‐6 in adult zebrafish brain

Liu

Bhattarai

Wang

et al. 2015

J of Comparative Neurology

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Cell adhesion molecule cadherins play important roles in both development and maintenance of adult structures. Most studies on cadherin expression have been carried out in developing organisms, but information on cadherin distribution in adult vertebrate brains is limited. In this study, we used in situ hybridization to examine mRNA expression of three cadherins, protocadherin-19, protocadherin-17 and cadherin-6 in adult zebrafish brain. Each cadherin exhibits a distinct expression pattern in the fish brain, with protocadherin-19 and protocadherin-17 showing much wider and stronger expression than that of cadherin-6. Both protocadherin-19 and protocadherin-17 expressing cells occur throughout the brain with strong expression in the ventromedial telencephalon, periventricular regions of the thalamus and anterior hypothalamus, stratum periventriculare of the optic tectum, dorsal tegmental nucleus, granular regions of the cerebellar body and valvula, and superficial layers of the facial and vagal lobes. Numerous sensory structures (e.g. auditory, gustatory, lateral line, olfactory and visual nuclei) and motor nuclei (e.g. oculomotor, trochlear, trigeminal motor, abducens and vagal motor nuclei) contain protocadherin-19 and/or protocadherin-17 expressing cell. Expression of these two protocadherins is similar in the ventromedial telencephalon, thalamus, hypothalamus, facial and vagal lobes, but substantially different in the dorsolateral telencephalon, intermediate layers of the optic tectum, and cerebellar valvula. In contrast to the two protocadherins, cadherin-6 expression is much weaker and limited in the adult fish brain.

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Inversion of layer‐specific cadherin expression profiles and maintenance of cytoarchitectonic areas in the allocortex of the reeler mutant mouse

Cited by 10 publications

References 62 publications

Expression profile of N‐cadherin and protocadherin‐19 in postnatal mouse limbic structures

Expression profile of N‐cadherin and protocadherin‐19 in postnatal mouse limbic structures

Laminar and Dorsoventral Molecular Organization of the Medial Entorhinal Cortex Revealed by Large-scale Anatomical Analysis of Gene Expression

Differential expression of protocadherin‐19, protocadherin‐17, and cadherin‐6 in adult zebrafish brain

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