Invertase‐catalyzed reactions in alcoholic solutions

Selisko, Barbara; Ulbrich, Renate; Schellenberger, Alfred; Müller, Ute

doi:10.1002/bit.260351008

Cited by 20 publications

(11 citation statements)

References 9 publications

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“…It catalyzes hydrolysis of sucrose to equimolar mixture of glucose and fructose. Furthermore, this enzyme can be used for synthesis of fructo-oligosaccharides [22], and it can be also applied for synthesis of alkyl β-fructosides using water-organic alcohol mixtures (methanol and ethanol) [23]. The S. cerevisiae cells produce two forms of invertase -the intracellular non-glycosylated and the extracellular ("external") heavily N-glycosylated form.…”

Section: Introductionmentioning

confidence: 99%

“…via reversed hydrolysis (thermodynamic control) or transglycosylation (kinetic control) [35,36]. In such a way, methyl β-D-fructofuranoside (MF) can be synthesized using invertase in a kinetically controlled transglycosylation process [23,37].…”

Section: Introductionmentioning

confidence: 99%

“…Increasing the concentration of methanol favours the alcoholysis according to reaction 2, shown in Fig. 2a, and it also disfavours hydrolysis of MF [23]. In order to achieve higher yields, the water activity has to be as low as possible.…”

Section: Introductionmentioning

confidence: 99%

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Increased yield of enzymatic synthesis by chromatographic selection of different N‐glycoforms of yeast invertase

et al. 2020

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Invertases are glycosidases applied for synthesis of alkyl glycosides that are important and effective surfactants. Stability of invertases in the environment with increased content of organic solvent is crucial for increase of productivity of glycosidases. Their stability is significantly influenced by N-glycosylation. However, yeast N-glycosylation pathways may synthesize plethora of N-glycan structures. A total natural crude mixture of invertase glycoforms (EINV) extracted from Saccharomyces cerevisiae was subfractionated by anionexchange chromatography on industrial monolithic supports to obtain different glycoforms (EINV1-EINV3). Separated glycoforms exhibited different stabilities in water-alcohol solutions that are in direct correlation with the amount of phosphate bound to N-glycans.Observed differences in stability of different invertase glycoforms were used to improve productivity of methyl β-D-fructofuranoside (MF) synthesis. The efficiency and yield of MF synthesis was improved more than 50% when the most stabile glycoform bearing the lowest amount of phosphorylated N-glycans is selected and utilized. These data underline the importance of analysis of glycan structures attached to glycoproteins, demonstrates different impact of N-glycans on the surface charge and enzyme stability in regard to particular reaction environment, and provide a platform for improvement of yield of industrial enzymatic synthesis by chromatographic selection of glycoforms on monolithic supports.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Increased yield of enzymatic synthesis by chromatographic selection of different N‐glycoforms of yeast invertase

et al. 2020

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“…Hydrolysis of sucrose by invertase can be controlled using methanol, ethanol and n‐ propanol (Selisko et al . ; Rodríguez et al . ).…”

Section: Methods To Store Nectarmentioning

confidence: 99%

A review of the methods for storing floral nectars in the field

Amato

Petit

2017

Plant Biol J

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The knowledge of floral nectar sugar characteristics, such as concentration, ratio and mass, is essential to understand the complex nature of nectar production and pollination systems. Although nectar is commonly stored in ecology, storage reliability and effectiveness have rarely been quantified. Inappropriate nectar storage between sampling and analysis can alter nectar chemistry as a result of enzyme or microbial action. Our review of the literature indicates that measures to preserve nectar sugars before analysis include refrigeration, freezing, the addition of an antimicrobial agent, spotting and drying on filter paper, the addition of a desiccant or a combination of these storage treatments. Nectar stored on filter paper is removed by washing with a solvent before analysis. Elution methods are often complex, not standardised and poorly reported in published work. Existing storage methods have generally been used without an evaluation of their impact on results, but evidence suggests a potentially large impact on result accuracy. Future studies should report storage treatments and elution methods to legitimise comparison among independent studies and provide unbiased evaluation of the results. In view of the wide range of storage methods used and lack of verification of their appropriateness, is imperative that standardised and effective methods be developed to ensure that results are reliable. We recommend the prompt analysis of nectar, detailed description of methods, including size of filter paper and method of elution, and use of sterile techniques.

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“…We immediately added HPLC‐grade methanol (50 μ l) to each vial of all three types of samples (EFN, honeydew, and phloem sap) to deactivate enzymes such as invertase (Selisko et al . , Rodríguez et al . ) whose activity might change sugar compositions, and also to inhibit microbial growth (Shenoy et al .…”

Section: Relative Percent Compositions and Concentrations Of Sugars Amentioning

confidence: 99%

Foliar Extrafloral Nectar of Humboldtia brunonis (Fabaceae), a Paleotropic Ant‐plant, is Richer than Phloem Sap and More Attractive than Honeydew

et al. 2014

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Invertase‐catalyzed reactions in alcoholic solutions

Cited by 20 publications

References 9 publications

Increased yield of enzymatic synthesis by chromatographic selection of different N‐glycoforms of yeast invertase

Increased yield of enzymatic synthesis by chromatographic selection of different N‐glycoforms of yeast invertase

A review of the methods for storing floral nectars in the field

Foliar Extrafloral Nectar of Humboldtia brunonis (Fabaceae), a Paleotropic Ant‐plant, is Richer than Phloem Sap and More Attractive than Honeydew

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