Investigating conjugated polymer nanoparticle formulations for lateral flow immunoassays

Schüller, Moritz; Meister, Annette; Green, Mark; Dailey, Lea Ann

doi:10.1039/d1ra05212h

Cited by 3 publications

(3 citation statements)

References 42 publications

(49 reference statements)

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“…In accordance with the previous report, the CPN absorption spectra were redshifted (CN-PPV) and blueshifted (PCPDTBT) compared to the one for the CP in THF (Figure S4 in the Supporting Information). These changes are generally associated with the alteration in the conformation of the polymer chain during nanoparticle formation. ,− …”

Section: Resultsmentioning

confidence: 99%

Multienergy Calibration Applied for the Quantification of Polymer Concentration in Conjugated Polymer Nanoparticles

et al. 2022

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Multienergy calibration (MEC) is an innovative analytical method to determine the analyte concentration in atomic spectroscopy. Recently, the MEC’s applicability for quantifying molecular species by ultraviolet–visible (UV–vis) absorption and fluorescence was demonstrated, showing higher precision and sensitivity in the determination of analyte concentration than the standard methods such as standard calibration and standard addition. In this work, MEC is shown to be a suitable method for quantifying the concentration of a conjugated polymer in conjugated polymer nanoparticles (CPNs). The proof of concept of this approach is demonstrated by analyzing CPNs containing poly(2,5-di(hexyloxy)cyanoterephthalylidene) and poly[2,6-(4,4-bis(2-ethylhexyl)-4H-cyclopenta-[2,1-b;3,4-b0]dithiophene)-alt-4,7-(2,1,3-benzothiadiazole)] using UV–vis absorption measurements. The results are comparable to those obtained with the conventional external calibration method but with improved limits of detection and quantification. Additionally, MEC is less labor-intensive because of the reduced need to prepare multiple calibration standards and allows aqueous sample analysis.

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Section: Resultsmentioning

confidence: 99%

Multienergy Calibration Applied for the Quantification of Polymer Concentration in Conjugated Polymer Nanoparticles

et al. 2022

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“…Schüller's team encapsulated two distinct Pdots varieties - poly-(9,9-dioctyl-fluorenyl-2,7-diyl) (PDOF) and poly-(2,5-di-hexyloxy-cyanoterephthalylidene) (CN-PPV) - within silica shell-crosslinked Pluronic© micelles (Si-NP). Their research focused on examining NP brightness relative to the concentration of conjugated polymers [ 258 ]. Remarkably, the detection sensitivity of Pdots in flow immunoassays (LFI) was significantly higher than that of Si-NPs and other reference nanoparticles, requiring considerably less Pdots mass for effective detection.…”

Section: Sensing and Detectionmentioning

confidence: 99%

“…In 2014, Taylor et al updated the quantitative densitometric analysis approach for western blots, detailing the complete workflow from sample preparation to data analysis [ 488 ]. Applications in basic experiments in medicine are also seen in the following work [ 258 , 489 , 490 ]. Ye et al, in 2015, achieved a detection limit at the single-picogram level in dot blots and detected minute quantities of proteins using conventional western blot techniques [ 307 ].…”

Section: Achievement Transformationmentioning

confidence: 99%

Semiconducting polymer dots for multifunctional integrated nanomedicine carriers

Zhang,

Yu,

et al. 2024

Materials Today Bio

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Biodistribution and Biotoxicity Assessment of Fluorescent Conjugated Polymer Dots

Ren,

Li,

Xiong

2024

Adv Healthcare Materials

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Conjugated polymer dots (Pdots) have shown potential in the biomedical fields due to their optical properties and customizable design. However, the limited research on the biotoxicity of Pdots hinders their further application and translation. Lipophilic Pdots are prone to adsorbing specific proteins, leading to targeted tissue accumulation. Therefore, lipophilic fluorescent Pdots (Bare‐Pdots) are synthesized using the conjugated polymer poly[2‐methoxy‐5‐(2′‐ethylhexyloxy)−1,4‐phenylenevinylene] (MEH‐PPV) to systematically evaluate their biodistribution and biotoxicity in stem cells, zebrafish embryos, and mice. It is observed that Bare‐Pdots are readily internalized by cells and adhered to the embryonic chorion. Additionally, Bare‐Pdots exhibit a distinct distribution in brown adipose tissue and heart, closely associated with phagocytosis of capillary endothelial cells involved in lipid metabolism. Notably, injection of Bare‐Pdots at 5 mg kg−1 results in dysfunction of brown adipose tissue and an increased risk of obesity 90 days post‐injection. Furthermore, hydrophilic COOH‐Pdots and NH2‐Pdots with reduced lipophilicity are synthesized using amphiphilic ligands. NH2‐Pdots show similar distribution but lower biotoxicity compared to Bare‐Pdots. Nevertheless, injection of COOH‐Pdots at 5 mg kg−1 causes a decrease in white blood cells and renal tubular damage. These findings provide valuable insights for optimizing dosage to ensure the safe use of Pdots in preclinical applications.

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Investigating conjugated polymer nanoparticle formulations for lateral flow immunoassays

Abstract: Conjugated polymer nanoparticles are sensitive signal transducers in lateral flow immunoassays.

Cited by 3 publications

References 42 publications

Multienergy Calibration Applied for the Quantification of Polymer Concentration in Conjugated Polymer Nanoparticles

Multienergy Calibration Applied for the Quantification of Polymer Concentration in Conjugated Polymer Nanoparticles

Semiconducting polymer dots for multifunctional integrated nanomedicine carriers

Biodistribution and Biotoxicity Assessment of Fluorescent Conjugated Polymer Dots

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