Investigating neuroprotective roles of Bacopa monnieri extracts: Mechanistic insights and therapeutic implications

Fatima, Urooj; Roy, Sonam; Ahmad, Shahnawaz; Al-Keridis, Lamya Ahmed; Alshammari, Nawaf; Adnan, Mohd; Islam, Asimul; Hassan, Md. Imtaiyaz

doi:10.1016/j.biopha.2022.113469

Cited by 28 publications

(9 citation statements)

References 133 publications

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“…The mean value of the IRS1 fold change after the addition of 50 μg of B. monnieri extract was noted to be 0.65. The mean value of the IRS1 fold change after the addition of 100 μg of B. monnieri extract was noted to be 0.91, which is almost similar to that of the initial untreated cells, hence proving the antihyperglycemic activity of the B. monnieri plant extract [ 28 ]. The mean value of the AKT fold change in the untreated cells was noted to be 1.0.…”

Section: Discussionmentioning

confidence: 92%

Analyzing the Antihyperglycemic Effect of Cissus quadrangularis and Bacopa monnieri on 3T3-L1 Cell Lines

IGK

et al. 2024

Cureus

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Background Cissus quadrangularis is a perennial shrub of the grape family. Other names for it include devil's backbone, veld grape, and pirandai (Tamil). Bacopa monnieri , a perennial plant, is native to wetlands in eastern and southern India. The 3T3-L1 cell line, which was created from 3T3 cells, was used in the scientific study. The current study's purpose is to evaluate the antihyperglycemic benefits of B. monnieri and C. quadrangularis , which will be added to the current arsenal of efficient herbal hypoglycemic medications. Aim To analyze and compare the anti-hyperglycaemic effects of the two plant extracts, C. quadrangularis and B. monnieri using a 3T3-L1 cell line. Materials and methods C. quadrangularis seeds were gathered, and extraction was conducted. The B. monnieri plant was harvested, and a rotary evaporator was used to extract the flower. Adipocyte cells were obtained from NCCS, Pune. A CO 2 incubator was used to incubate the cells. The MTT assay and gene expression analysis were done on the cell line samples. Results The antihyperglycemic effects of C. quadrangularis IRS mRNA levels of 0.7 and AKT mRNA levels of 0.7 are compared to B. monnieri IRS1 mRNA levels of 0.6 and AKT mRNA levels of 0.6 to build better diabetic treatments. The antihyperglycemic benefits of C. quadrangularis levels of IRS mRNA and AKT mRNA are compared to the influence of B. monnieri IRS1 mRNA and AKT mRNA on the development of better diabetic drugs. Conclusion Comparing the effects of C. quadrangularis and B. monnieri on the 3T3 cell line by gene expression of IRS mRNA and AKT mRNA suggests that the particular AKT downregulation shows that insulin suppresses gluconeogenesis and C. quadrangularis inhibits hyperglycemia in 3T3-L1 cells, while research on in vitro rats suggests that B. monnieri may minimize the signs and symptoms of diabetes via enhancing IRS1/AKT signaling.

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Section: Discussionmentioning

confidence: 92%

Analyzing the Antihyperglycemic Effect of Cissus quadrangularis and Bacopa monnieri on 3T3-L1 Cell Lines

IGK

et al. 2024

Cureus

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“…Data from a meta-analysis study showed that plant adaptogens could provide significant benefits in the treatment of chronic fatigue, cognitive impairment, and immune protection under stressful conditions [ 14 ]. BM showed neuroprotective properties in several in vitro and in vivo models [ 29 ]; particularly, it inhibited microgliosis in a mice model of dementia and bacosides [ 30 ], and counteracted the cognitive impairment in age-related chronic neuroinflammation in rat [ 31 ]. A randomized clinical study performed by Morgan and Stevens observed that the same bacopa extract used in this work at the dose of 300 mg/day for 4 months improved older adults’ memory performance [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Novel Combination of Choline with Withania somnifera (L.) Dunal, and Bacopa monnieri (L.) Wetts Reduced Oxidative Stress in Microglia Cells, Promoting Neuroprotection

Borgonetti,

Galeotti

2023

IJMS

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Memory deficit is one of the major negative outcomes of chronic stress. Cholinergic system modulates memory not only through the neuronal cells, but also via interactions with non-neuronal cells, suggesting that microglia can influence synaptic function and plasticity, contributing to cognition and memory function. Withania somnifera (L.) Dunal (WS) and Bacopa monnieri (L.) Wettst (BM), are traditional herbal medicinal products used for the temporary relief of symptoms of stress. The aim of this study was to investigate whether choline (CLN) activity could be enhanced via an association with adaptogens: WS and BM extracts. First, we optimized an in vitro model of corticotropin-releasing hormone (CRH)-induced oxidative stress on microglial BV2 cells. CRH 100 nM reduced BV2 cell viability and induced morphological changes and neurotoxicity after 24 h of microglia stimulation. Moreover, it induced an increase in the production of reactive oxygen species (ROS) and dysregulated antioxidant protein (i.e., SIRT-1 and NRF-2). The association between choline and adaptogens (CBW) 10 μg/mL counteracted the effect of CRH on BV2 cells and reduced the neurotoxicity produced by BV2 CRH-conditioned medium in the SH-SY5Y cell lines. CBW 200 mg/kg produced an ameliorative effect on recognition memory in the novel object recognition test (NORT) test in mice. In conclusion, combining choline with adaptogen plant extracts might represent a promising intervention in chronic stress associated with memory disturbances through the attenuation of microglia-induced oxidative stress.

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“…Similarly, naringenin could alleviate the neurotoxic effects of Aβ in vitro as it has been associated with the downregulation of the expression of APP and BACE, reducing amyloidogenesis, and decreasing the level of phosphorylated tau [82]. Several other lines of evidence also support the beneficial effects of narigenin in AD and PD [83] as well as the neuroprotective effects of Bacopa monnieri extract, with potential therapeutic applications in AD [84].…”

Section: Neuroinflammation and Microglia Dysregulated Genes In Ad And...mentioning

confidence: 94%

Underlying Mechanisms of Brain Aging and Neurodegenerative Diseases as Potential Targets for Preventive or Therapeutic Strategies Using Phytochemicals

Abdolmaleky

Zhou

2023

Nutrients

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During aging, several tissues and biological systems undergo a progressive decline in function, leading to age-associated diseases such as neurodegenerative, inflammatory, metabolic, and cardiovascular diseases and cancer. In this review, we focus on the molecular underpinning of senescence and neurodegeneration related to age-associated brain diseases, in particular, Alzheimer’s and Parkinson’s diseases, along with introducing nutrients or phytochemicals that modulate age-associated molecular dysfunctions, potentially offering preventive or therapeutic benefits. Based on current knowledge, the dysregulation of microglia genes and neuroinflammation, telomere attrition, neuronal stem cell degradation, vascular system dysfunction, reactive oxygen species, loss of chromosome X inactivation in females, and gut microbiome dysbiosis have been seen to play pivotal roles in neurodegeneration in an interactive manner. There are several phytochemicals (e.g., curcumin, EGCG, fucoidan, galangin, astin C, apigenin, resveratrol, phytic acid, acacetin, daucosterol, silibinin, sulforaphane, withaferin A, and betulinic acid) that modulate the dysfunction of one or several key genes (e.g., TREM2, C3, C3aR1, TNFA, NF-kb, TGFB1&2, SIRT1&6, HMGB1, and STING) affected in the aged brain. Although phytochemicals have shown promise in slowing down the progression of age-related brain diseases, more studies to identify their efficacy, alone or in combinations, in preclinical systems can help to design novel nutritional strategies for the management of neurodegenerative diseases in humans.

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Investigating neuroprotective roles of Bacopa monnieri extracts: Mechanistic insights and therapeutic implications

Cited by 28 publications

References 133 publications

Analyzing the Antihyperglycemic Effect of Cissus quadrangularis and Bacopa monnieri on 3T3-L1 Cell Lines

Analyzing the Antihyperglycemic Effect of Cissus quadrangularis and Bacopa monnieri on 3T3-L1 Cell Lines

Novel Combination of Choline with Withania somnifera (L.) Dunal, and Bacopa monnieri (L.) Wetts Reduced Oxidative Stress in Microglia Cells, Promoting Neuroprotection

Underlying Mechanisms of Brain Aging and Neurodegenerative Diseases as Potential Targets for Preventive or Therapeutic Strategies Using Phytochemicals

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