Investigating oligonucleotide hybridization at subnanomolar level by surface plasmon resonance biosensor method

Abstract

We have optimized surface plasmon resonance (SPR) biosensor technology for a rapid, direct, and low-consumption label-free multianalyte screening of synthetic oligonucleotides (ONs) with modified internucleotide linkages potentially applicable in antisense therapy. Monitoring of the ONs hybridization is based on the formation of complex between the natural oligonucleotide probe immobilized on the sensor surface and the ON in solution in contact with the sensor surface. An immobilization chemistry utilizing the streptavidin-biotin interaction was employed to obtain desired ligand density and high hybridization efficiency. It was demonstrated that the sensor is capable of detecting complementary 23-mer ONs in concentrations as low as 0.1 nM with high specificity and reproducibility.

Functionalizable low-fouling coatings for label-free biosensing in complex biological media: advances and applications

“…For example, HS(CH 2 ) 11 EG n with n=2-6 can be relatively easily synthesized or purchased from a variety of sources. In summary, SAMs are suitable for use in biosensor and microarray applications involving buffer solutions or diluted complex media (<~15 %) [15,[104][105][106]. The lowest fouling from blood plasma on SAMs was obtained for (CH 2 ) 11 EG 6 SAMs ( Table 1).…”

High-throughput SPR sensor for food safety

“…The reported detection limit is also better than the best LODs obtained using high-performance SPR sensors based on spectroscopy of surface plasmons (Persson et al, 1997;Vaisocherová et al, 2006).…”

A dual surface plasmon resonance assay for the determination of ribonuclease H activity

“…The sensor is based on the attenuated total reflection method and wavelength spectroscopy of surface plasmons (Vaisocherová et al, 2006). In this sensor, white light is used to excite surface plasmons on a thin gold film in four areas which are interfaced with four separate chambers of a four-channel flow-cell.…”