Investigating the Effect of Endurance Training and Supplementation of Persia Rosa Extract on Mitochondrial Apoptosis of Myocardium

Abstract

Background & objectives:Physical training and some supplements through different mechanisms could be effective in reducing apoptosis; the aim of this study was to investigate the effect of endurance training and supplementation of Persia Rosa extract on mitochondrial apoptosis of myocardium. Methods: In this experimental study, 52 male rats were randomly assigned into five homogeneous groups, three-month control, six months control, Persia Rosa supplement, endurance training and endurance training+Persia Rosa. Training groups participated in endurance training protocol for 12 weeks. The Persia Rosa and endurance training+Persia Rosa groups received 1 gram Persia Rosa extract per kg body weight. After the implementation of the training protocol and consumption of the extract, the surgical and cardiac tissue extraction procedures were performed. The data were analyzed by ANOVA test and Bonferroni post hoc tests at the significant level of less than 5% using SPSS-22 software. Results: The results showed that Bax/Bcl-2 ratio and Caspase-3 gene expression were significantly decreased in endurance training and endurance training+Persia Rosa groups to the control group (p˂0.05). Bax/Bcl-2 showed a significant decrease in the Persia Rosa supplement group (p˂0.05), but Caspase-3 was not significant in the Persia Rosa supplement group. Conclusion:It is expected that the effect of endurance training with consumption of Persia Rosa extract on Bax/Bcl-2 ratio and Caspase-3 will provide a good platform for caspase mechanisms and apoptosis processes in the myocardium of rats.

The combined effect of eight-week resveratrol supplementation and interval training on some regulatory and executive factors of hepatocyte apoptosis in male rats with diabetes

“…This discussion can partly depend on the activation of cytosolic c-Jun N-terminal kinase (JNK); thus, JNK inhibits Bcl-2 protein by being phosphorylated by cellular stress stimuli. Inside the mitochondria, JNK increases the permeability of the mitochondrial membrane, causing the release of pro-apoptotic factors such as the apoptotic-inducing factor and cytochrome C, thereby triggering the caspase cascade (41,42). Another way to inhibit apoptosis and increase cell lifespan is the relationship between exercise and resveratrol, as well as silent information regulator 1 proteins, which increase during exercise.…”

The effect of endurance exercise and rosehip extract supplementation on the expression of P53 and cytochrome C genes in male rat heart

“…The PCR temperature pattern for the respective genes was 95°C for 10 min for the first cycle, which continued with 45 cycles in two 95°C stages for 20 seconds and 60°C for 60 seconds. To analyze the data, first, the ΔCt of the gene in each sample was calculated as the difference between Ct of the corresponding gene and GAPDH gene as a reference gene 16 . Reactions were performed in triplicate, and expression levels were calculated using the CT comparative method (2−ΔΔ C T) 5 .…”

“…It seems that endurance training reduces apoptosis in skeletal and cardiac muscles through the effect on mitochondrial membrane permeability, and has also been shown to improve antioxidant capacity in patients with chronic heart failure. 5 , 14 - 16 Therefore, it increases the expression of cell death factors, inflammation, and immunological changes in the blood. 17 It has been shown that levels of P53 and cytochrome C proteins increase after exercise, 18 and exercise positively regulates the P53 gene, thereby encouraging damaged cells to repair the damage, or induce self-destruction if repair is not possible.…”