Investigating the first outbreak of oriental theileriosis in cattle in South Australia using multiplexed tandem PCR (MT-PCR)

Gebrekidan, Hagos; Gasser, Robin B.; Perera, Piyumali K.; McGrath, Sarah; McGrath, Sean; Stevenson, M.A.; Jabbar, Abdul

doi:10.1016/j.ttbdis.2015.04.012

Cited by 18 publications

(33 citation statements)

References 18 publications

(68 reference statements)

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“…However, it was higher than that (0.68%; 3/439) reported recently in cattle from Egypt (Elsify et al, 2015). MT-PCR also revealed a lower prevalence (3.9%) of T. orientalis infection in Ethiopia compared with those estimated in recent studies following outbreaks of oriental theileriosis in cattle from Australia (53.8 to 75.5%) and New Zealand (99.4%) using the same assay (Gebrekidan et al, 2015;Perera et al, 2015b,c). The variation in prevalence of T. orientalis among different countries could be due to numerous factors, including the presence and abundance of ticks (e.g., Haemaphysalis spp.)…”

Section: Resultscontrasting

confidence: 68%

“…However, recently, T. orientalis complex, transmitted mainly by Haemaphysalis spp. caused significant morbidity, economic losses and/or mortality in cattle in the Asia-Pacific region (Izzo et al, 2010;Aparna et al, 2011;Kamau et al, 2011;McFadden et al, 2011;Perera et al, 2013Perera et al, , 2014Gebrekidan et al, 2015). Based on the sequence analysis of the major piroplasm surface protein (MPSP) gene, two (chitose and ikeda) of the 11 currently known genotypes (called chitose or type 1, ikeda or type 2, buffeli or type 3, types 4 to 8, and N1 to N3) of T. orientalis are considered to be pathogenic, leading to disease (oriental theileriosis) (reviewed by Sivakumar et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

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Molecular characterization of Theileria orientalis from cattle in Ethiopia

Gebrekidan

Gasser

Baneth

et al. 2016

Ticks and Tick-borne Diseases

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This study reports the first molecular characterization of Theileria orientalis in local breeds of cattle in Ethiopia. A conventional PCR utilizing major piroplasm surface protein (MPSP) gene and an established multiplexed tandem PCR (MT-PCR) were used to characterize T. orientalis and to assess the infection intensity, respectively. Of 232 blood samples tested, T. orientalis DNA was detected in only 2.2% of samples using conventional PCR; two genotypes buffeli (1.3%; 3/232) and type 5 (0.9%; 2/232) of T. orientalis were detected. Phylogenetic analysis revealed that the buffeli MPSP sequences from Ethiopia were closely related to those reported from Kenya, Sri Lanka and Myanmar, and type 5 sequences from Ethiopia grouped with those from Korea, Japan, Vietnam and Thailand. A higher number of samples (3.9%; 9/232) were test-positive by MT-PCR and four genotypes (buffeli, chitose, ikeda and type 5) of T. orientalis were detected. The average intensity of infections with genotypes buffeli (DNA copy numbers 11,056) and type 5 (7508) were significantly higher (P<0.0001) than the pathogenic genotype ikeda (61 DNA copies). This first insight into T. orientalis from cattle in Ethiopia using MPSP gene provides a basis for future studies of T. orientalis in various agroclimatic zones and of the impact of oriental theilerosis on cattle in this and other countries of Africa.

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Section: Resultscontrasting

confidence: 68%

Section: Introductionmentioning

confidence: 99%

Molecular characterization of Theileria orientalis from cattle in Ethiopia

Gebrekidan

Gasser

Baneth

et al. 2016

Ticks and Tick-borne Diseases

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“…Additionally, we did not find any discrepancy in the assignments of three genotypes (buffeli, chitose, and ikeda) of T. orientalis using the MPSP gene or the p23 gene (6,16). However, sequence variation in the p23 gene region identified in this study is likely due to population variation within T. orientalis (buffeli) in blood samples from cattle and buffaloes from locations outside Victoria, Australia (New South Wales and South Australia within Australia, and Ethiopia, New Zealand, and Pakistan) (7)(8)(9)(10)(11)(12); at the time, the p23 gene had not been assessed for variation within T. orientalis outside Victoria, Australia (16).…”

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confidence: 64%

Inadequate Differentiation of Theileria orientalis Genotypes buffeli and ikeda in a Multiplexed Tandem PCR (MT-PCR) Assay Using the p23 Gene as a Marker

2017

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“…Blood samples (n = 265) from cattle were available from previous studies conducted in Australia [17][18]. Extracted DNA of all of these samples had been individually tested for T. orientalis infection by MT-PCR.…”

Section: Blood Samples and Dna Extractionmentioning

confidence: 99%

“…To overcome these limitations, Perera et al [17] developed a multiplexed-tandem PCR (MT-PCR) assay for the simultaneous detection and differentiation of the four commonest genotypes (buffeli, chitose, ikeda and type 5) of the T. orientalis complex in Australasia and their quantitation in bovine blood samples. This assay proved to be an invaluable tool for the routine diagnosis, epidemiological studies and for the exploration of theileriosis outbreaks [10,[18][19][20].…”

Section: Introductionmentioning

confidence: 99%

Assessing the performance of multiplexed tandem PCR for the diagnosis of pathogenic genotypes of Theileria orientalis using pooled blood samples from cattle

Gebrekidan

Gasser

Stevenson

et al. 2017

Molecular and Cellular Probes

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Oriental theileriosis caused by multiple genotypes of Theileria orientalis is an important tick-borne disease of bovines. Here, we assessed the performance of an established multiplexed tandem PCR (MT-PCR) for the diagnosis of the two recognized, pathogenic genotypes (chitose and ikeda) of T. orientalis in cattle using pooled blood samples. We used a total of 265 cattle blood samples, which were divided into two groups according to previous MT-PCR results for individual samples. Samples in group 1 (n = 155) were from a herd with a relatively high prevalence of T. orientalis infection; and those in group 2 (n = 110) were from four herds with a low prevalence. For group 1, 31 and 15 batches of five- and ten-pooled samples (selected at random), respectively, were formed. For group 2, 22 and 11 batches of five- and ten-pooled samples (selected at random), respectively, were formed. DNAs from individual pooled samples in each batch and group were then tested by MT-PCR. For group 1, the apparent prevalences estimated using the 31 batches of five-pooled samples (97%) and 15 batches of ten-pooled samples (100%) were significantly higher compared with individual samples (75%). For group 2, higher apparent prevalences (9% and 36%) were also recorded for the 22 and 11 batches of pooled samples, respectively, compared with individual samples (7%). Overall, the average infection intensity recorded for the genotypes of chitose and ikeda were considerably lower in pooled compared with individual samples. The diagnostic specificities of MT-PCR were estimated at 95% and 94%, respectively, when batches of five- and ten-pooled samples were tested, and 94% for individual samples. The diagnostic sensitivity of this assay was estimated at 98% same for all individual, five- and ten-pooled samples. This study shows that screening batches of five- and ten-pooled blood samples from cattle herds are similar to those obtained for individual samples, and, importantly, that the reduced cost for the testing of pooled samples represents a considerable saving to herd managers.

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Investigating the first outbreak of oriental theileriosis in cattle in South Australia using multiplexed tandem PCR (MT-PCR)

Cited by 18 publications

References 18 publications

Molecular characterization of Theileria orientalis from cattle in Ethiopia

Molecular characterization of Theileria orientalis from cattle in Ethiopia

Inadequate Differentiation of Theileria orientalis Genotypes buffeli and ikeda in a Multiplexed Tandem PCR (MT-PCR) Assay Using the p23 Gene as a Marker

Assessing the performance of multiplexed tandem PCR for the diagnosis of pathogenic genotypes of Theileria orientalis using pooled blood samples from cattle

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