Investigating the glycosylation of normal and ovarian cancer haptoglobins using digoxigenin-labelled lectins

Kaţnik, I; Jadach, J.; Krotkiewski, Hubert; Gerber, J

doi:10.1007/bf00919295

Cited by 28 publications

(19 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, in the 3rd trimester the synthesized sialic acid by the mother, crosses the placenta to contribute to the foetal growth [19]. In contrast to foetal tissues, the glycoproteins derived from plasma of adult persons, are known to contain larger amounts of glycans terminated by α2-6 linked sialic acid [17,22]. Moreover, at the turn of 2nd and 3rd trimesters relative amounts of glycans terminated by α1-6 innermost fucose attached to the core region of N-glycan increased.…”

Section: Discussionmentioning

confidence: 99%

“…The positive controls were the following: asialo-haptoglobin preparation derived from ovarian cancer fluid [17] for AAA, LTA, UEA, transferrin for SNA (Boehringer Mannheim, from "Glycan Differentation Kit"), mouse glycophorin for MAA (a gift from dr hab. Hubert Krotkiewski, Institute of Immunology and Therapiae Experimantalis, PAN, Wroc law, Poland), respectively.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Relative amounts of sialic acid and fucose of amniotic fluid glycoconjugates in relation to pregnancy age

et al. 2005

View full text Add to dashboard Cite

The present knowledge concerning the glycan structures and role of glycoconjugates derived from amniotic fluid is fragmentary and mainly focuses on the individual glycoproteins. The question has arisen as whether the general glycosylation pattern of amniotic fluid glycoconjugates can change with the progression of a normal pregnancy. In the present work we have described the dynamic, quantitative alterations in relative amounts of sialic acid and fucose linked by a variety of anomeric linkages to subterminal oligosaccharide structures of amniotic fluid glycoconjugates in relation to pregnancy age. The analysis was performed in the following groups of amniotic fluids derived from normal pregnancy by lectin dotting method: "2nd trimester" (14-19 weeks), "3rd trimester" (29-37 weeks), "perinatal period" (38-40 weeks) , "delivery at term" (39-41 weeks) and "post date pregnancy" (41-43 weeks). In the "3rd trimester" the amniotic fluid glycoconjugates contained higher relative amounts of glycans terminated by alpha2-6-linked sialic acid (p < 0.00002) and by alpha1-6 innermost fucose (p < 0.000001) than those in the 2nd trimester. In contrast, they showed the lower relative amount of fucose linked alpha1-3 (p < 0.02). At the perinatal period the relative amount of alpha2-6-linked sialic acid increased (p < 0.03), and it then decreased during delivery (p < 0.02) to the level found in the "3rd trimester" group. In the post date pregnancy all parameters studied increased. The sialyl- and fucosyl-glycotopes of the amniotic fluid glycoconjugates may play an critical role in growth and tissue remodeling of the foetus, as well as may might reflect maturation of a foetus. Additionally, a determination of the glycotope expressions might be helpful in prenatal diagnosis as predictor factors for well being of mother and child.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Relative amounts of sialic acid and fucose of amniotic fluid glycoconjugates in relation to pregnancy age

et al. 2005

View full text Add to dashboard Cite

show abstract

“…The carbohydrate moiety of haptoglobin from ascitic fluids of patients with ovarian carcinoma was apparently changed as judged from the reactions with haemoglobin and concanavalin A (17,18). Investigations of ovarian cancer haptoglobins using digoxigenin-labelled lectins suggest that normal and ovarian cancer haptoglobins differ in their content of carbohydrate structures containing sialic acid-linked a(2-6) or a(2-3) to galactose and in the type of glycan branching (9).…”

Section: Resultsmentioning

confidence: 99%

Measurements of Haptoglobin by the Reaction with Concanavalin A in Sera of Patients with Ovarian Tumours

Kaţnik¹,

Jadach²,

Kmieciak³

et al. 1995

CCLM

Self Cite

View full text Add to dashboard Cite

Summary:The concentration of haptoglobin in sera of healthy women and patients with non-malignant and malignant ovarian tumours was measured by two methods, i.e. complex formation with haemoglobin and complex formation with concanavalin A. High correlation (r = 0.74) between both the methods was found in the group of healthy women, but correlation coefficients were much lower in the group of non-malignant and malignant tumours (r = 0.42 and 0.37, respectively). Direct determinations of haptoglobin, and calculation of the ratio of haptoglobin bound to concanavalin A to haptoglobin bound to haemoglobin revealed statistically significant differences among the examined groups. Comparison of two methods of haptoglobin quantitation suggest that processes connected with ovarian disorders may alter the glycosylation of haptoglobin oligosaccharide chains.

show abstract

“…The positive control for MAA, SNA, UEA, LTA, and LCA was a native haptoglobin and an asialo-haptoglobin preparation derived from ovarian cancer fluid. 25 The negative control was a human albumin preparation (Sigma) included in the test instead of milk and plasma samples. The background absorbance was low (< 0.05 absorbance units [AU]) when Tris-buffered saline was included in the tests instead of (1) lectin, (2) ExtrAvidinalkaline phosphatase (AP), and (3) milk or plasma samples.…”

Section: Lectin-agp Elisa For Differentiating Sialyl and Fucosyl Glycmentioning

confidence: 99%

Lactation Stage-Related Expression of Sialylated and Fucosylated Glycotopes of Human Milk α-1-Acid Glycoprotein

Orczyk-Pawiłowicz

Hirnle

Berghausen-Mazur

et al. 2014

Breastfeeding Medicine

View full text Add to dashboard Cite

Background: Because terminal sugars of a-1-acid glycoprotein (AGP) are reported to be involved in antiinflammatory and immunomodulatory processes, their expressions might have an influence on the proper function of immune system of newborns. Here, relative amounts of sialylated and fucosylated glycotopes on human milk AGP over normal lactation were investigated. Materials and Methods: AGP concentration and relative amounts of its sialylated and fucosylated glycovariants were analyzed in early colostrum, colostrum, and transitional and mature milk samples of 127 healthy mothers by lectin-AGP enzyme-linked immunosorbent assay using a2,3-and a2,6-sialic acid and a1,2-, a1,3-, and a1,6-fucose specific biotinylated Maackia amurensis, Sambucus nigra, Ulex europaeus, Tetragonolobus purpureus, and Lens culinaris lectins, respectively. Results: AGP concentration in human milk was about 30 times lower than in plasma of lactating mothers and decreased gradually over lactation. Milk AGP showed significantly higher expression of sialylated and fucosylated glycotopes in comparison with those of plasma AGP. Milk AGP glycovariants containing a2,6-sialylated and a1,6-and a1,2-fucosylated glycotopes showed the highest relative amounts in early colostrums. With progression of lactation, the expressions of glycotopes a1,2-fucosylated decreased starting from Day 4 and those of a2,6-sialylated and a1,6-fucosylated from Day 8 of lactation, whereas the level of a2,3-sialyl-glycotope was almost constant over 45 days of lactation. In contrast, the expression of a1,3-linked fucose on AGP was low in colostrums and significantly higher in transitional and mature milk. Conclusions: The relative amounts of sialylated and fucosylated glycovariants of human hindmilk AGP significantly varied between Days 2 and 45 of normal lactation.

show abstract

Investigating the glycosylation of normal and ovarian cancer haptoglobins using digoxigenin-labelled lectins

Cited by 28 publications

References 20 publications

Relative amounts of sialic acid and fucose of amniotic fluid glycoconjugates in relation to pregnancy age

Relative amounts of sialic acid and fucose of amniotic fluid glycoconjugates in relation to pregnancy age

Measurements of Haptoglobin by the Reaction with Concanavalin A in Sera of Patients with Ovarian Tumours

Lactation Stage-Related Expression of Sialylated and Fucosylated Glycotopes of Human Milk α-1-Acid Glycoprotein

Contact Info

Product

Resources

About