Investigating the intracellular bactericidal effects of rifampicin loaded S-protected thiomeric chitosan nanocargoes against Mycobacterium tuberculosis

Rauf, Aisha; Razzaq, Sobia; Tabish, Tanveer A.; Tahseen, Sabira; Sandhu, Mansoor Abdullah; Shahnaz, Gul

doi:10.1016/j.jddst.2020.102184

Cited by 8 publications

(4 citation statements)

References 31 publications

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“…While SLNs prepared through other techniques such as solvent evaporation show significant convolution in FTIR spectra. Other studies also reported similar conclusion from FTIR analysis [46]. SIM characteristic peaks of FTIR can be seen in the formulation indicating no change in chemical structure of drug.…”

Section: Ftir Analysissupporting

confidence: 73%

Hydrogel Containing Solid Lipid Nanoparticles Loaded with Argan Oil and Simvastatin: Preparation, In Vitro and Ex Vivo Assessment

Khan

Rehman

Howari

et al. 2022

Gels

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Transdermal hydrogels have the potential to improve therapeutic outcomes via enhancing bioavailability and reducing toxicity associated with oral delivery. The goal of the present study was to formulate and optimise argan oil loaded transdermal hydrogel containing lipid nanoparticles. The high pressure homogenization (HPH) method was utilised to fabricate Simvastatin loaded solid lipid nanoparticles (SIM-SLNs) with precirol ATO 5 as a lipid core and Poloxamer 407 (P407) to stabilise the core. The optimised nanoformulation was characterised for its particle diameter, zeta potential, surface morphology, entrapment efficiency, crystallinity and molecular interaction. Furthermore, transdermal hydrogel was characterised for physical appearance, rheology, pH, bio adhesion, extrudability, spreadability and safety profile. In vitro and ex vivo assays were executed to gauge the potential of SLNs and argan oil for transdermal delivery. The mean particle size, zeta potential and polydispersity index (PDI) of the optimised nanoparticles were 205 nm, −16.6 mV and 0.127, respectively. Crystallinity studies and Fourier transform infrared (FTIR) analysis revealed no molecular interaction. The in vitro release model explains anomalous non-Fickian release of drug from matrix system. Ex vivo skin penetration studies conducted through a fluorescence microscope confirmed penetration of the formulation across the stratum corneum. Hydrogel plays a crucial role in controlling the burst release and imparting the effect of argan oil as hypolipidemic agent and permeation enhancer.

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Section: Ftir Analysissupporting

confidence: 73%

Hydrogel Containing Solid Lipid Nanoparticles Loaded with Argan Oil and Simvastatin: Preparation, In Vitro and Ex Vivo Assessment

Khan

Rehman

Howari

et al. 2022

Gels

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“…Toxicity of RIF (1 mol %)-Loaded MO-DOTAP (1 mol %) Cubosomes. The cellular toxicity of MO, MO-DOTAP (1 mol %) and MO-DOTAP-RIF at six different lipid concentrations (5,10,15,20,50, and 100 μg/mL) was tested using the MTS assay against the A549 epithelial lung cancer cell line over a 72 h time period (Figure 5). MO cubosomes were nontoxic (>95% cell viability) against A549 cells, consistent with that reported in earlier research.…”

Section: Size Surface Charge and Morphology Of Nanoparticles Followin...mentioning

confidence: 99%

“…3,4 Difficulties in the transport of conventional antibiotics across the plasma membrane to the site of infection, in combination with natural macrophage defense mechanisms, can markedly reduce the efficacy of current treatments. 5 A thick layer of mycolic acid present on the MTB cell wall presents an additional, very effective barrier to the delivery of hydrophobic antitubercular agents such as rifampicin, assisting survival of the bacteria. 6 Of 41 known new antibiotics currently at various stages of development, none are designed to treat intracellular infections specifically.…”

Section: ■ Introductionmentioning

confidence: 99%

Cubosome Lipid Nanocarriers As a Drug Delivery Vehicle for Intracellular Mycobacterium tuberculosis Infections

Sarkar

Dyett

Lakic

et al. 2023

ACS Appl. Mater. Interfaces

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Mycobacterium tuberculosis (MTB) causes the infectious disease tuberculosis (TB), responsible for more deaths than any other single infectious disease in history. Intracellular MTB are slow growing and difficult to target with traditional antitubercular drugs, leading to the emergence of multidrug resistance in TB infection, which is a major global public health issue. Recent advances in innovative lipid nanotechnologies for drug delivery have demonstrated promising outcomes for chronic infectious diseases but have not yet been tested as potential delivery systems for intracellular infections such as TB. The current study evaluates the potential of monoolein (MO)-based cationic cubosomes for the encapsulation and delivery of the first line antitubercular drug rifampicin (RIF) against an MTB-H37Ra in vitro culture model. In particular, we show that the use of cationic cubosomes as delivery vehicles reduced the minimum inhibitory concentration (MIC) of RIF by 2-fold against actively replicating MTB-H37Ra (compared to that of the free drug) and also shortened the lifecycle duration of axenic MTB-H37Ra from 5 to 3 days. The cubosome-mediated delivery was also found to be effective against intracellular MTB-H37Ra within THP-1 human macrophages, with a 2.8 log reduction in viability of the bacilli after 6 days incubation at the MIC. The killing time was also reduced from 8 to 6 days without distressing the host macrophages. Mechanistic studies on the uptake of RIF-loaded cationic cubosomes using total internal reflection fluorescence microscopy (TIRFM) demonstrated the capacity of these lipid particles to effectively target intracellular bacteria. Overall, these results demonstrate that cationic cubosomes are a potent delivery system for the antitubercular drug RIF for therapeutic management of TB.

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“…Proficient targeting with the eradication of Tuberculosis [204] Mannose linked thiolated nanocarriers A promising intracellular targeting approach to overcome the multi-drug resistance of anti-tuberculosis drugs [205] MTC-PLGA-PM nanocarriers Enhanced macrophage uptake and parasite killing intracellularly against VL.…”

Section: Pei Conjugated Nanomicellesmentioning

confidence: 99%

Nanotechnology for Therapy of Zoonotic Diseases: A Comprehensive Overview

et al. 2022

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Zoonotic infections belong to multiple infectious diseases transferred from animals to humans. Now, the treatment and diagnosis of zoonotic infections are perplexing due to genetic mutations, target site modifications, and multi‐drug resistance. Despite their benefits, most diagnostic molecular techniques have certain limits in terms of repeatability and sensitivity, mainly due to the heterogeneity among the diverse family of zoonotic pathogens. Therefore, developing more efficient and cost‐effective theranostics tools is the need of the hour to address these concerns. For this purpose, nanotechnology has revolutionized medicine with versatile potential capabilities for diagnosing and treating zoonosis via the targeted and controlled delivery of antimicrobial drugs via binding to the overexpressed infectious macrophages. Massive advancements have been made in fabricating novel nano‐based formulations to control zoonosis based on the use of poly(ethylenimine)‐conjugated nanomicelles, mannosylated thiolated chitosan (MTC)‐coated PM‐loaded PLGA NPs, mannose linked thiolated nanocarriers, adjuvanted pDNA hydrogel, arginine‐based nanocarriers, quantum dots to treat and diagnose a wide range of zoonotic diseases, including zoonotic influenza, salmonellosis, leishmaniasis, rabies, brucellosis, Lyme Disease, tuberculosis, and other infections caused by West Nile Virus, emerging coronaviruses (SARS, MERS, COVID‐19), in a preferentially targeted way. Recently developed anti‐pathogen loaded‐nanoformulations with enhanced cellular uptake, biocompatibility, and hemocompatibility have shown the ability to cross biological barriers when orally administrated. Therefore, this article reviewed the latest milestones and future growth areas in the field of efficient theranostics platforms to manage zoonotic infections.

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Investigating the intracellular bactericidal effects of rifampicin loaded S-protected thiomeric chitosan nanocargoes against Mycobacterium tuberculosis

Cited by 8 publications

References 31 publications

Hydrogel Containing Solid Lipid Nanoparticles Loaded with Argan Oil and Simvastatin: Preparation, In Vitro and Ex Vivo Assessment

Hydrogel Containing Solid Lipid Nanoparticles Loaded with Argan Oil and Simvastatin: Preparation, In Vitro and Ex Vivo Assessment

Cubosome Lipid Nanocarriers As a Drug Delivery Vehicle for Intracellular Mycobacterium tuberculosis Infections

Nanotechnology for Therapy of Zoonotic Diseases: A Comprehensive Overview

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