Investigating the sperm parameters, oxidative stress and histopathological effects of salvia miltiorrhiza hydroalcoholic extract in the prevention of testicular ischemia reperfusion damage in rats

Davoodi, Farshid; Taheri, Shayan; Raisi, Abbas; Rajabzadeh, Asghar; Ahmadvand, Hassan; Hablolvarid, M.H.; Zakian, Amir

doi:10.1016/j.theriogenology.2020.01.002

Cited by 20 publications

(24 citation statements)

References 48 publications

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“…It has been reported that chemoattracted neutrophils stimulated by TNF‐α can produce massive amounts of ROS when migrating towards the testes, resulting in DNA fragmentation and lipid peroxidation (Gurocak et al., 2011; Shimizu et al., 2016). In line with our findings, multiple studies have shown that torsion/detorsion results in an increase in lipid peroxidation (measured by MDA) and a decrease in the level of antioxidant enzymes (Davoodi et al., 2020; Elshaari, Elfagih, Sheriff, & Barassi, 2011). One study reported that the intraperitoneal administration of MC at a dose of 30 mg/kg is able to reduce neuropathic pain in a rat model of chronic constriction injury, thereby mitigating the inflammatory response and lowering the rate of oxidative stress (MDA) (Abbaszadeh et al., 2018).…”

Section: Discussionsupporting

confidence: 92%

Minocycline attenuates testicular damages in a rat model of ischaemia/reperfusion (I/R) injury

et al. 2020

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Testicular torsion is one of the most significant urological disorders in which the spermatic cord is twisted along the longitudinal axis and affects the male gender at any age; however, newborns, children and adolescent boys are the main target of the disease (Fehér & Bajory, 2016). Epidemiological studies indicated that testicular torsion occurs between the ages of 13 and 16 years and/or during the first year of life (Pogorelic, Mrklic, Juric, Biocic, & Furlan, 2013). The incidence of the spermatic cord torsion, in males with the age range of 1-25 years old, is estimated at 4.5 per 100,000 (Pogorelic et al., 2013). In this pathological condition, the blood flow to testes is considerably decreased, causing severe scrotal pain and tissue ischaemia. As widely accepted, both the duration and degree of twisting are the two most essential factors involved in testicular tissue damage. Therefore, early diagnosis and surgical interventions (i.e. testicular detorsion to retrieve the normal blood flow to the organ) are effective strategies to prevent (a) testicular injury, (b) loss of testicular germ cells and (c) finally infertility (Mellick, Sinex, Gibson, & Mears, 2019). In this regard, if the surgical procedure is carried out within 6 hr of pain onset, it would be feasible to save testicles by 90%-100%, while 12 and 24 hr after torsion, the survival rate of testicles would be reduced by 50% and 0%-10% respectively (

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Section: Discussionsupporting

confidence: 92%

Minocycline attenuates testicular damages in a rat model of ischaemia/reperfusion (I/R) injury

et al. 2020

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“…The ability of AD to induce repair and recovery of the germinal epithelium can be associated to its phytochemical content especially polyphenols, as other medicinal plants containing polyphenols have demonstrated ameliorative potentials on toxicityinduced damage in the testes. [49][50][51] This is also supported by the report of Khaki and colleagues 41 on the ability of quercetin to attenuate loss of germinal epithelium and vacuolization of germ cells. The potentials of AD to prevent germinal epithelial loss and improve sperm quality and functions may provide a natural alternative and therapy in attenuating diabetes-related reproductive dysfunctions in males.…”

Section: Discussionsupporting

confidence: 53%

<p>The Beneficial Role of <em>Anchomanes difformis</em> in STZ-Induced Reproductive Dysfunction in Male Wistar Rats</p>

Alabi¹,

Villiers²,

Plessis³

et al. 2020

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Background: Progression of diabetes mellitus has increasingly led to several diabetic complications. Diabetes is one of the major factors implicated in male reproductive system damage. Recent approaches such as the use of medicinal plants have been explored in the management of diabetes and associated complications. Anchomanes difformis (common name: children's umbrella) has been shown to possess anti-diabetic ability in animal model. Therefore, this study seeks to investigate the potency of Achomanes difformis in ameliorating diabetes-induced reproductive dysfunction. Methods: Type 2 diabetes was induced in male Wistar rats with 10% fructose administration for 2 weeks and an intraperitoneal injection of 40mg/kgBW of streptozotocin. Aqueous extract (200mg and 400mg/kgBW) of Anchomanes difformis leaves was administered daily for 6 weeks. The rats were randomly divided into 7 groups with a minimum of eight rats in each (8 rats in normal groups and 10 in diabetic groups). The impact of diabetes and treatment was investigated by estimating sperm concentration, motility indices, viability and morphological parameters in the normal, treatment controls and diabetic rats using CASA-SCA system. Histological examination of the testes and epididymis was performed. Results: Diabetes induction resulted in significant decrease in sperm concentration, viability and some motility parameters with 40% abnormalities in sperm morphology. The administration of Anchomanes difformis significantly increased sperm concentration and sperm viability, while it significantly improved the percentage of morphologically normal sperm in diabetic rats. Anchomanes difformis ameliorated testicular damage such as vacuolization and loss of germinal epithelium in the diabetic-treated rats when compared to the diabetic controls. Conclusion: The potency Anchomanes difformis displayed against diabetic-induced damage in the reproductive system might be a new and promising tool in the management of male reproductive dysfunctions and associated complications in diabetes mellitus.

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“…The use of herbs to treat diseases in humans and animals is growing in many countries [ 7 ]. According to the World Health Organization (WHO), about 80% of people worldwide trust traditional medicine and the use of alternative and complementary medicine, including herbal extracts [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

The effect of aqueous extract of Prunus dulcis on tibial bone healing in the rabbit

et al. 2021

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Background Bone fractures are medical emergencies that require prompt intervention to help return bone to its normal function. Various methods and treatments have been utilized to increase the speed and efficiency of bone repair. This study aimed to investigate the treatment effects of Prunus dulcis aqueous extract on tibial bone healing in rabbits. Methods All animals were distributed in five groups with six rats in each group, including the sham group, the control group in which tibial lesion was made and received distilled water, treatment groups with 150 mg kg−1, 300 mg kg−1 doses of Prunus dulcis extract, and osteocare treated group. Biochemical blood factors including calcium, phosphorus, and alkaline phosphatase (on days 0, 10, 30, and 50), biomarkers of oxidative stress such as GPx, CAT, and MDA (on days 10 and 30), radiological evaluation, histopathological parameters, and osteocalcin immunohistochemical expression were assessed. Results The data showed calcium levels in the treatment groups increased significantly from day 10 to day 50, respectively, and blood phosphorus levels decreased from day 10 to day 50 in the treatment groups. Alkaline phosphatase initially increased and then decreased in treatment groups. In the treatment groups, GPx and CAT levels significantly increased, and the serum amount of MDA reduced. The best antioxidant results were related to the extract-treated group with a higher dose. Radiographic score was significantly higher in the treatment groups than the control group on day 30. Based on the pathological findings, the healing occurred faster in the extract-treated group with a higher dose. Osteocalcin expression was significantly higher in the control group than that in the treatment groups. Conclusions Treatment with Prunus dulcis extract with a dosage of 300 mg/kg accelerated tibial bone healing in rabbits. Graphical abstract

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Investigating the sperm parameters, oxidative stress and histopathological effects of salvia miltiorrhiza hydroalcoholic extract in the prevention of testicular ischemia reperfusion damage in rats

Cited by 20 publications

References 48 publications

Minocycline attenuates testicular damages in a rat model of ischaemia/reperfusion (I/R) injury

Minocycline attenuates testicular damages in a rat model of ischaemia/reperfusion (I/R) injury

<p>The Beneficial Role of <em>Anchomanes difformis</em> in STZ-Induced Reproductive Dysfunction in Male Wistar Rats</p>

The effect of aqueous extract of Prunus dulcis on tibial bone healing in the rabbit

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