Investigation and optimization of formulation parameters on preparation of targeted anti-CD205 tailored PLGA nanoparticles

Abstract

The purpose of this study was to assess the effect of various formulation parameters on anti-CD205 antibody decorated poly( d , l -lactide co-glycolide) (PLGA) nanoparticles (NPs) in terms of their ability to target dendritic cells (DCs). In brief, emulsification solvent evaporation technique was adapted to design NP formulations using two different viscosity grades (low and high) of both ester and carboxylic acid terminated PLGA. Incorporation of ligand was achieved following physical adsorption or chemical conjugation processes. The physicochemical characterizations of formulations were executed to assess the effects of different solvents (chloroform and ethyl acetate), stabilizer percentage, polymer types, polymer viscosities, ligand-NP bonding types, cross-linkers, and cryoprotectants (sucrose and trehalose). Modification of any of these parameters shows significant improvement of physicochemical properties of NPs. Ethyl acetate was the solvent of choice for the formulations to ensure better emulsion formation. Infrared spectroscopy confirmed the presence of anti-CD205 antibody in the NP formulation. Finally, cytotoxicity assay confirmed the safety profile of the NPs for DCs. Thus, ligand modified structurally concealed PLGA NPs is a promising delivery tool for targeting DCs in vivo.

Development and characterization of polo-like kinase 2 loaded nanoparticles-A novel strategy for (serine-129) phosphorylation of alpha-synuclein

“…Finally, PLGA 502H was selected for further experiments. The influence of the type of polymer on the size of PLGA-NPs has been previously described in the literature (Haddadi and Jahan, 2015;Mundargi et al, 2008). These studies suggest that an increase in polymer viscosity also results in an increased particle size.…”

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“…Although it was reported in the works of literature that increasing the concentration of PVA was usually associated with a decrease of PZ or PDI,42,43 the PZ, PDI and ζ-potential of NP 5 were not significantly affected by the increase of PVA concentration (unpaired t- test, P > 0.05). It could be related to that once the optimum packing of PVA and the minimum droplet size were reached, the higher concentrations of PVA would not play any further role with regard to PZ 44…”

“…Aggregation of non-cryoprotected NPs may be generated by many stresses of lyophilization that could destabilize colloidal suspension especially, the stress of freezing and dehydration. Hence, trehalose at concentration of 5% was used as a cryoprotectant for the subsequent preparation of NPs 42,44,49. Trehalose is considered as one of the most compatible cryoprotectant due to its amorphous nature that causes minimal damage.…”