Investigation into Seasonal Effect and Browning Inhibitor on Callus Regeneration of Seedless Barberry (Berberis vulgaris var. asperma)

Abstract

Leaves, one year old stems and buds were used as explants. WPM, B5, MS, half and one-fourth strength MS with 2,4-?D, NAA, IBA, IAA and picloram 2, 5 and 10 mg/l were used for callus induction. Investigation into the seasonal effect on callus regeneration and explants browning was carried out in mid-April until September. Results showed that washing with sterile distilled water for one hour after surface sterilization was the best treatment for browning inhibition of explants than other treatments. Leaf explants have best callus formation. MS with 8 g/l agar had strong effect on callus formation. Culture for callus formation in May and June found to be higher than other months. 2,4-D, NAA and 10 mg/l picloram significantly enhanced callus formation. Leaf and bud explants sampling in April to May did not show any endophyte contamination but leaf explants in June until September, specially the one year old stems, showed intensive contamination. Key words: Seasonal effect, Browning inhibitor, Berberis vulgaris D. O. I. 10.3329/ptcb.v21i2.10239 Plant Tissue Cult. & Biotech. 21(2): 161-168, 2011 (December)

Effects of black Berberis vulgaris L combined with aerobic and resistance exercise on blood metabolic parameters and liver enzymes in obese rats

“…It is worth highlighting that none of both training methodologies Supporting our results, Berberis vulgaris is recognized within the scientific body of knowledge as one of the functional foods that could be beneficial for the management of hyperlipidemia and chronic inflammation in humans, having a strong protective effect on the cardiovascular system (Changizi Ashtiyani et al, 2013;Emamat et al, 2020;Fatehi et al, 2005). Previous expert literature reported that the intake of Berberis vulgaris can reduce lipid profile and liver enzymes in serum (Changizi Ashtiyani et al, 2013;Emamat et al, 2020;Fatehi et al, 2005;Lazavi et al, 2018;Iloon Kashkooli et al, 2015;Mohammadi et al, 2014;Mohammadi et al, 2011;Shidfar et al, 2012;Taheri et al, 2012;Vrzal et al, 2005). The antioxidant activity…”

The First Successful Report: Control of Browning Problem in in vitro Culture of Iranian Seedless Barberry, a Medicinally Important Species

“…As an efficient technique, micropropagation has been successfully used for propagation of many plants. Unlike many fruit trees, whose tissue culture have been successfully optimized, in vitro culture of Iranian seedless barberry has not been successful thus far, as disappointingly reported by Azizi et al (2007) and Mohammadi et al (2011). One of the most serious obstacles in the way of successful micropropagation of Iranian seedless barberry is the exudation of phenolic compound.…”

Seasonal variation in contamination and browning of Acacia nilotica nodal explants in vitro

“…The success of in vitro clonal propagation of trees has been restricted mainly due to their recalcitrant nature and other associated problems, such as heavy inborne as well as surface-bound infection, leading to less survival frequency and oxidation of phenolic substances leached out from the cut ends of explants. Seasonal variations in temperature, light intensity, humidity, and pathogen pressure can significantly influence these parameters, affecting the overall outcomes of tissue culture experiments (Mohammadi et al, 2011;Martini et al, 2013;Devi and Yadav, 2022). Elevated temperatures and increased humidity during certain seasons create favourable conditions for the proliferation of microbial contaminants such as bacteria, fungi, and yeasts (Alagarsamy et al, 2018;Huang et al, 2019).…”