2019
DOI: 10.3390/ijerph16193611
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Investigation of a Gas Hydrate Dissociation-Energy-Based Quick-Freezing Treatment for Sludge Cell Lysis and Dewatering

Abstract: A gas Hydrate dissociation-energy-based Quick-Freezing treatment (HbQF) was applied for sewage sludge cell rupture and dewatering. Carbon dioxide (CO2) and water (H2O) molecules in sewage create CO2 gas hydrates, and subsequently the sludge rapidly freezes by releasing the applied pressure. Cell rupture was observed through a viability evaluation and leachate analysis. The decreased ratios of live cell to dead cells, increased osmotic pressure, and increased conductivity showed cell lysis and release of electr… Show more

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Cited by 3 publications
(3 citation statements)
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“…The disinfection effect can be evaluated with a live/dead bacterial staining kit (L7012, InvitrogenTM, Waltham, MA, USA), which is used for staining live or dead microbes in the sludge sample. The differences in fluorescence of live and dead cells are based on the permeability of dyes (e.g., DAPI/PI) through cell membranes (Kim et al, 2019) (Santos et al, 2013).…”
Section: економічні інноваціїmentioning
confidence: 99%
See 2 more Smart Citations
“…The disinfection effect can be evaluated with a live/dead bacterial staining kit (L7012, InvitrogenTM, Waltham, MA, USA), which is used for staining live or dead microbes in the sludge sample. The differences in fluorescence of live and dead cells are based on the permeability of dyes (e.g., DAPI/PI) through cell membranes (Kim et al, 2019) (Santos et al, 2013).…”
Section: економічні інноваціїmentioning
confidence: 99%
“…The effect of the rapid freezing method, using the endothermic reaction of the CO 2 gas hydrate dissociation, applied to sludge cell lysis on the viability of microorganisms in sludge was investigated by (Kim et al, 2019). A gas Hydrate dissociation-energy-based Quick-Freezing treatment (HbQF) was applied for SS cell rupture and dewatering.…”
Section: економічні інноваціїmentioning
confidence: 99%
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