INVESTIGATION OF ACTIVE COMPOUND IN CLOVE (Syzygium aromaticum) EXTRAC AND COMPARED WITH INHIBITORS OF GROWTH OF SOME TYPES OF BACTERIA CAUSING FOOD POISONING

Karm, Ibtisam Fareed Ali

doi:10.36103/ijas.v50i6.855

Cited by 11 publications

(9 citation statements)

References 12 publications

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“…Table (1) shows that eugenol in clove extract was found as a major component with 32.5 mg/L followed by gallic acid with 24.8 mg/L, kaempferol with 12.8 mg/L, and vanillic acid 9.8 mg/L. Investigation of clove extract by Karm (14) proved that clove extract content of eugenol was the best antimicrobial agent, particularly with the high doses. In this aspect, Lee and Shibamoto (18) reported that the whole essential oil of clove or its main extract component is eugenol C6H12O2; 4-ally 1-2methoxy phenol).…”

Section: Resultsmentioning

confidence: 99%

HPLC Analysis and Antifungal Activity of Some Plant Extracts Against Decay Apple Fruits

YOUSIF¹,

HASSAN²

2023

Iraqi J. Agric. Sci.

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This study was aimed to determine the qualitative and quantitative phenolic compounds. Results revealed that HPLC analysis identified Hydroxy derivatives of benzoic acids, hydroxy-cinnamic acids, in addition to flavonoids. The major compounds detected in clove were eugenol 32.5 mg/L, while in thyme the predominant phenol was gallic acid 35.9 mg/L. Quercetin was the greatest phenolic compound in both eucalyptus and sage 49.8 and 55.6 mg/L, respectively. Clove extract inhibited all selected pathogens entirely when applied at 20%. Subsequently, thyme extract at the same concentration inhibited the growth of B. cinerea completely. Eucalyptus extract revealed a lowest inhibition with 23.2%, 11.83%, and 7.83% after three, six, and nine days, respectively. P. griseofulvum showed remarkable susceptibility to extracts due to 51.99% growth inhibition followed by 25.33% and 20.67% for both A. alternata and B. cinerea, respectively.

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Section: Resultsmentioning

confidence: 99%

HPLC Analysis and Antifungal Activity of Some Plant Extracts Against Decay Apple Fruits

YOUSIF¹,

HASSAN²

2023

Iraqi J. Agric. Sci.

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“…The column temperature was maintained at 25 °C. The injection volume of the sample solution was adjusted at 20 μL, wavelength λ 210 nm [42] …”

Section: Methodsmentioning

confidence: 99%

“…The injection volume of the sample solution was adjusted at 20 μL, wavelength λ 210 nm. [42] Calculation of concentration of the sample Sample concentration ¼ Area of sample Area of standrd � concentration of standard� dilution factor…”

Section: Analyzation Of Eugenol By High-performance Liquid Chromatogr...mentioning

confidence: 99%

Synergistic Antimicrobial Activity of Eugenol in Combination with Fosfomycin to Combat Escherichia coli and Potential Effect on Plasmid‐Mediated Fosfomycin Resistance Genes

Attaallah Ibrahim,

Kadhim Mohammed

2023

Chemistry & Biodiversity

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The presence of multidrug‐resistant pathogenic microorganisms makes it challenging to cure bacterial illnesses. Syzygium aromaticum has been used for medicinal purposes since ancient times. The objective of this study was to investigate the potential synergistic effect of the combination of Eugenol and Fosfomycin against clinically Uropathogenic Escherichia coli (UPEC) and their possible co‐treatment as well as their contribution to plasmid‐mediated Fosfomycin resistance (fosA3 and fosA4) genes using molecular assays. Eugenol was extracted from clove (Syzygium aromaticum) plants using steam distillation by Clevenger and analyzed by high‐performance liquid chromatography (HPLC). UPEC accounted for 63.6% of all isolates. Specifically, 99.3% of the UPEC isolates exhibited resistance to multiple types of antibiotics [multidrug‐resistant (MDR)]. The MIC for Eugenol was 1.25‐5 µg/mL, and Fosfomycin was 512‐1024 µg/mL, while the MBC for Eugenol was 5‐10 µg/mL and Fosfomycin was 2048 µg/mL. The synergistic effects were considerable, with 1/4 MIC of Eugenol resulting in 1/8 MIC Fosfomycin. Eugenol inhibited most of the UPEC isolates at 4‐8 hours, Fosfomycin at 8‐12 hours, and co‐treatment at 4‐8 hours. The fosA3 and fosA4 genes were detected in 5.7% and 2.9% of the isolates, respectively. The results showed variable gene expression changes in response to the different treatments.

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“…These techniques differ in design base and output, experimental conditions such as temperature, solvent, time, etc. towards getting better extraction results in quality and quantity beside cost and eco-friendly environmental aspects [9][10][11][12][13][14][15].…”

Section: Figure (1) Clove (Syzygium Aromaticum L)mentioning

confidence: 99%

Perforated Tube vs. Soxhlet Apparatus: A New Extraction Tool Confirming by Clove Sample and GC-Mass Analysis

Khalaf¹

2022

IJOIR

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In this paper, a new efficient extraction tool of bioactives from plant parts was designed, applied, and compared with Soxhlet apparatus. This tool was a Quickfit®- Pyrex® glass tube perforated easily inserted in round flask containing solvent. Clove buds in both non-grinded and grinded form were analyzed by GC-Mass. Our observations were gotten from designing and using perforated tube compared to Soxhlet apparatus for Clove buds’ extraction. It is a simplest tool in designing and handling than Soxhlet apparatus that ensured more contact between Clove buds in their bag and used solvent than Soxhlet. Employing bag sewed from face mask is low cost than high quality Soxhlet - cellulose thimble. Furthermore, this sewed bag can be discharged from its content, cleaned, and reused but expensive cellulose thimble is made for one use. Perforated tube presents a good choice for efficient-short extraction time, minimum sample weight and solvent volume resulting higher quantity of Clove bioactives compared to Soxhlet. By analytical foundations, using grinded Clove gave different qualitative-quantitative analysis of the tested bioactives in the extracted solution compared to non- grinded buds. Eugenol was the major constituent in non- grinded buds while grinded powder gave 2-methoxy-3-(2-propenyl)-phenol as a maximal bioactive. This new tool may be used with less solvent quantity for better continuous extraction series however Soxhlet must be used with more than half flask volume to get fast-efficient extraction. Also, Soxhlet needs direct heating source however perforated tube may be applied in both direct heating and water bath. These notes are important in using low boiling solvent (volatile) for better extraction. Because extraction is not limited to plant parts, this new tool may be used as a general extraction tool. So to guarantee getting higher quantities of volatile constituents with easier steps, perforated tube is an excellent choice.

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INVESTIGATION OF ACTIVE COMPOUND IN CLOVE (Syzygium aromaticum) EXTRAC AND COMPARED WITH INHIBITORS OF GROWTH OF SOME TYPES OF BACTERIA CAUSING FOOD POISONING

Cited by 11 publications

References 12 publications

HPLC Analysis and Antifungal Activity of Some Plant Extracts Against Decay Apple Fruits

HPLC Analysis and Antifungal Activity of Some Plant Extracts Against Decay Apple Fruits

Synergistic Antimicrobial Activity of Eugenol in Combination with Fosfomycin to Combat Escherichia coli and Potential Effect on Plasmid‐Mediated Fosfomycin Resistance Genes

Perforated Tube vs. Soxhlet Apparatus: A New Extraction Tool Confirming by Clove Sample and GC-Mass Analysis

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