Antimicrobial properties of Syzygium aromaticum were investigated and phytochemical active groups were done, clove oil contain a unique active compound known as eugenol was extracted and isolated to be tested. Four different pathogenic bacterial strains (Staphylococcus auras, Listeria monocyogenes, Salmonella typhimurium and Escherichia coli) were obtained and isolated from contaminated food, three concentrations for the plant (eugenol extract) were prepared to be a treatments, T1, T2 and T3 with percentage of 100%, 50%, 25% respectively. Antimicrobial activity was measured by using disc diffusion method and the best treatment was T1, which tested for eugenol qualitative and quantitative activity that was determined by using (HPLC). The results showed that the inhibition zones were increased with the increasing of concentrations of eugenol extract thus it was very clearly that the results encourage the use of natural Sources like some plants or some parts of plants to solve some problems done by bacteria activities that cause food poisoning. .
This study was carried out to isolate, identified and study two types of Iraqi fresh water algae Scenedesmus sp. and Oscellatoria sp also determine some of their active compounds that play an important role as antioxidants and antimicrobial againsts some bacteria. Three types of bacteria Salmonella sp, Escherichia coli and Staphylococcus sp that cause spoilage for food were tested. Antimicrobial activity of ethanolic extracts for both algae were examined toward tested bacteria. Four treatments were prepared which were [S.Ex(T1) ,O.Ex(T2), S.Ex: Of*(T3), 1:1 O.Ex: Of*(T4) 1:1],and (C) as control treatment with the antibiotic only (Oflaxacin). Disk diffusion method was used to test the antibacterial activity. Some active compounds indicate and high performance liquid chromatography (HPLC) technique used to determine some of them . Both algae contain some active ingredients that consider to be the reason for their ability as antimicrobials. The results of this study showed that the highest inhibition zones were in the treatments T3 and T4 compared to control treatment that means the synergistic activity for both antibiotic and the algae extract as alternative sources gave a better result for health and food protection.
Issues of antibiotic-resistant microorganisms have become a genuine wonder and needs to discover arrangements viable with the peril it addresses. Therefore, scientists have arisen calling to go towards regular other options and sources given commonly by nature. The study focused on the synergistic effectiveness of the cold (E1) and hot (E2) aqueous extracts of the leaves of two plants, Camellia sinensis, and Moringa oleifera, compared with (E3) which is control treatment, subsequent to researching some dynamic and active mixtures in the two plants separates utilizing phytochemical investigation and infrared spectroscopy analysis (FTIR). In addition, their utilization as anti-infection agents to delay the growth of some positive and negative isolated pathogenic bacteria that cause food contamination, by measuring the inhibition zone for bacteria growth, and determine the MIC assay for concentrates. The outcomes showed that the main mixtures combinations that were subjectively analyzed by FTIR were phenols, polyphenols, alkaloids, flavonoids, and other significant mixtures. Results showed that there were significant differences among the bacterial species towards each concentrate, just as clear critical differences among the pre-arranged concentrates towards every one of the confined bacterial species.
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