The Effect of Some Fresh  Water Algea Extracts in the Inhibition of the Growth of Some Microorganisim That Cause Food Spoilage

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Antimicrobial properties of Syzygium aromaticum were investigated and phytochemical active groups were done, clove oil contain a unique active compound known as eugenol was extracted and isolated to be tested. Four different pathogenic bacterial strains (Staphylococcus auras, Listeria monocyogenes, Salmonella typhimurium and Escherichia coli) were obtained and isolated from contaminated food, three concentrations for the plant (eugenol extract) were prepared to be a treatments, T1, T2 and T3 with percentage of 100%, 50%, 25% respectively. Antimicrobial activity was measured by using disc diffusion method and the best treatment was T1, which tested for eugenol qualitative and quantitative activity that was determined by using (HPLC). The results showed that the inhibition zones were increased with the increasing of concentrations of eugenol extract thus it was very clearly that the results encourage the use of natural Sources like some plants or some parts of plants to solve some problems done by bacteria activities that cause food poisoning. .

Section: Introductionmentioning

confidence: 99%

INVESTIGATION OF ACTIVE COMPOUND IN CLOVE (Syzygium aromaticum) EXTRAC AND COMPARED WITH INHIBITORS OF GROWTH OF SOME TYPES OF BACTERIA CAUSING FOOD POISONING

Karm¹

2019

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“…Also final extract composition depends on extraction solvent used for extract preparation, mainly because of its polarity. Moreover the contents of total flavonoids vary among myrtle parts (2,17,19). The main groups of phenolic compounds are flavonoids, phenolic acids, tannins, stilbenes and lignans (22).…”

Section: Results and Discussion Isolation Of Trichophyton Mentagrophytesmentioning

confidence: 99%

Evaluation of Myrtus Communis Flavonoid as Antider-Matophtic and Keratinase Inhibitior

Аббас¹

2020

This study was applied for extraction flavonoid compounds from Myrtus communis (aerial part)and detection of its effectiveness on growth of one of dermatiphytic isolates and keratinase activity, Trichophyton mentagrophytes was the most common isolates which was obtained with frequency (66%), the identification and quantitation of flavonoids was carried out by thin layer and high performance liquid chromatography. The results showed that each 100 g of aerial part of the plant contained 850 mg total flavonoids as rutin).The HPLC chromatogram for M. communis flavonoid extract revealed that, M. communis flavonoid extract contained Rutin, Quercetin, Apigenin, Kaempferol, Luteolin . The inhibitory effect of flavonoid against Trichophyton mentagrophytes and keratinase activity was evaluted. The higher effect was observed at concentration 10 mg/ml against fungal isolate .The minimum inhibiter concentration was at 3mg/ml while the higher inhibition of keratinase activity was obtained at concentration 5mg/ml with 25% remaining enzyme activity.

“…In this experiment Broth micro dilution method was performed, in two fold dilutions of antibiotics were done in broth media and broth was inoculated with 10 6 CFU/ml of the tested organisms (2). After incubation for (18)(19)(20)(21)(22)(23)(24) hrs, the MIC was determined as concentration in which no visible growth was observed (4).…”

Section: Minimum Inhibitory Concentrationmentioning

confidence: 99%

STUDY THE ANTIBACTERIAL ACTIVITY OF AQUEOUS EXTRACTION OF ONION (Allium cepaL) AGAINST Staphylococcus aureus ISOLATED FROM OTITIS MEDIA.

al.¹

2019

This study was aimed to determine the antibacterial activity of onion (Allium cepaL.) aqueous extract against S. aureus bacteria isolated from patients with otitis media. Twenty swabs samples were taken from patients with otitis infection; these samples were cultured on different agar media .Results revealed that half of these isolates were related to S. aureus and the five of these isolates were subject to sensitivity test against several antibiotic groups. Most of isolates 100% were resistance to amoxicillin, chloramphenicol, cefoxitin and pencillin, and 60 % of were showed resistance to cefotaxtime and tetracycline, where as 20 % were resistance to Ciprofloxacin. Depending on the results of antibiotic sensitivity, the two isolates(S2,S5) were chosen for determining the minimum inhibitory concentration (MIC) test, S2 isolate showed high-level of resistance at (16 and 128 µg/ml) to chloraemphenicol, amoxicillin, ampicillin and Cefotaxime respectively, while S5 at concentration (32, 64 and 128 µg\ml) were resistance to amoxicillin, ampicillin, chloraemphenicol and cefotaxtime. The MIC of onion extract against S. aureus was determined at two incubation periods The results were indicated that after 24 hrs, MIC of extract at 64 µg/ml was affected in inhibit the growth of S2 isolate, While 32 µg/ml was inhibited the growth of S5 isolate.However, after 72 hrs, It was found that the MIC at 32, 64 and 128 µg/ml were more effective in inhibited the growth of S2 and S5 isolates.