Investigation of Antioxidant and Rosmarinic Acid Variation in the Sage Collection of the Genebank in Gatersleben

Abstract

The total phenolic and flavonoid contents and 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reduction antioxidant power (FRAP) antioxidant capacities of 19 accessions of Salvia officinalis from the sage collection of the genebank in Gatersleben (Germany) were evaluated. The major phenolic compounds of sage, that is, rosmarinic acid, caffeic acid, carnosol, and carnosic acid, were quantified by reverse-phase high-performance liquid chromatography. The aerial parts of different individual plants of each accession were collected in two consecutive years from the same experimental field at the beginning of their flowering period. The results demonstrated a high variability between accessions. A general decreasing tendency from 2007 to 2008 was observed in most of the estimated parameters, that is, total phenolic, total flavonoid, rosmarinic acid, and caffeic acid contents and DPPH antioxidant activity. A slight opposite trend was obtained with the FRAP antioxidant capacity. Low but variable quantities of carnosol and carnosic acid were evaluated in the sample extracts. Individual plants within accessions were identified with high phenolic content and strong antioxidant activity. The rosmarinic acid content showed up to 8-fold differences between the lowest and the highest values. Overall, the study demonstrated a high variability in secondary metabolites present in sage, which could be used for breeding of highly antioxidative genotypes of S. officinalis.

Hairy roots of Dracocephalum moldavica: rosmarinic acid content and antioxidant potential

“…UPLC analysis of RA Analysis was performed using an Acquity Ultra Performance Liquid Chromatograph (Waters) equipped with a photodiode array, a UV-visible detector and an autoinjector, according to the method described by Lamien-Meda et al (2010). Separation was performed on a Shield RP C18-column (2.1 9 100 mm, 1.7 lm pore size) at a column temperature of 35°C.…”

Optimization of ultrasound-assisted extraction of phenolic compounds, antioxidants and rosmarinic acid from perilla leaves using response surface methodology

“…Moreover, rosmarinic acid (RA) has been shown to be the main biologically active polyphenolic compound in perilla leaves (Liu et al, 2013). RA is a well-known hydroxycinnamic acid ester that has interesting biological properties beneficial to human health, including antioxidant, anti-inflammatory, anticancer, and anti-allergenic activities (Lamien-Meda et al, 2010;Tang et al, 2014), and preventing food spoilage (Petersen & Simmonds, 2003;Pérez-Tortosa et al, 2012). Recently, interest has increased considerably in naturally occurring antioxidants for use in foods or medicinal materials as replacements for synthetic antioxidants such as BHA (beta hydroxy acid) and BHT (butylated hydroxy toluene), whose use is restricted due to safety concerns (Ito et al, 2005;Zheng & Wang, 2001).…”

Rosmarinic Acid Accumulation and Antioxidant Potential of Dracocephalum moldavica L. Cell Suspension Culture

“…UPLC analysis of RA Analysis was performed using an Acquity Ultra Performance Liquid Chromatograph (Waters) equipped with a photodiode array, a UV-visible detector and an autoinjector, according to Lamien-Meda et al (2010). Separation was performed on a Shield RP C18-column (2.19 × 100 mm, 1.7 lm pore size) at a column temperature of 35 °C.…”