Investigation of Association between oipA and iceA1/iceA2 Genotypes of Helicobacter pylori and Gastric Cancer in Iran

Abstract

Background: H pylori is the main causative agent of Gastric cancer and chronic gastritis. Genetic diversity of H. pylori has major contribution in its pathogenesis. We investigated the prevalence of oipA and iceA1/iceA2 positive strains of H. pylori among patients with gastric cancer and gastritis. Materials and Methods: Sampling performed by means of endoscopy from 86 patients. DNA was extracted from tissue samples using DNA extraction kit. PCR assay was performed and products were monitored by Agarose Gel Electrophoresis. Results: Urease Test and 16S rRNA PCR did not show significant differences in detection of H. pylori. The frequency of iceA1 allele in patients with gastric cancer was significantly higher than those with gastritis (p<0.05). However, there was no significant difference in prevalence of oipA and iceA2 genes among the two groups of patients (p>0.05). Conclusions: The iceA1 gene, but the oipA and iceA2 genes , is associated with H. pylori-induced gastric cancer. However, confirmatory studies must be performed in future.

Low Rate of babA2 Genotype among Iranian Helicobacter pylori Clinical Isolates

“…In the survey of Yamaoka Y et al, [5], oipA positive isolates from the US and Colombian patients were significantly recognized in duodenal ulcers (88%) and gastric cancer (89%) subjects. Several studies in Iran showed different ranges for prevalence of oipA from 40.8% to 95.9% [26][27][28][29][30]. Nevertheless, in none of these studies, except two of them [29,30] an association has been found between the genes and clinical outcome.…”

Prevalence of Specific Helicobacter Pylori CagA, VacA, IceA, UreC Genotypes and its Clinical Relevance in the Patients with Acid-Peptic Diseases

“…The iceA gene (a gene induced by contact with gastric epithelium) produces high levels of cytokine IL-8 in gastric mucosa and hold higher rates of peptic ulcer disease [9]. Molecular methods like polymerase chain reaction have the potential to accurately determine both the presence of infection and the genotype of bacteria; and looks very permissive as well as sensitive and specific test for the detection of H. pylori genotypes [9].…”

Role ofHelicobacter pyloriinfection in pathogenesis of gastric carcinoma

“…Nevertheless, this situation is not universal worldwide, for example, s1/m1 strains in districts of Asia is irrelevant to clinical outcome [82,83] . Additionally, investigation of the prevalence of oipA and iceA1/iceA2 positive strains among patients suffering from GC or gastritis results in that the frequency of iceA1 allele in patients with GC is significantly higher than those with gastritis [72] . However, there is no significant difference in prevalence of oipA and iceA2 genes among the two groups of patients (P > 0.05), suggesting the iceA1 gene might take a role in pathogenesis of H. pyloriinduced GC [72] .…”

“…Additionally, investigation of the prevalence of oipA and iceA1/iceA2 positive strains among patients suffering from GC or gastritis results in that the frequency of iceA1 allele in patients with GC is significantly higher than those with gastritis [72] . However, there is no significant difference in prevalence of oipA and iceA2 genes among the two groups of patients (P > 0.05), suggesting the iceA1 gene might take a role in pathogenesis of H. pyloriinduced GC [72] . Studies also indicated that certain genetic types of H. pylori hopQ were closely related to GC [84] .…”

“…Genetic diversity of H. pylori has major contribution in the pathogenesis [72] . Studies have been conducted focusing on polymorphism of the main virulence factors, such as cagA, vacA, oipA, iceA and hopQ.…”