Investigation of autotrophic, heterotrophic, and mixotrophic modes of cultivation on lipid and biomass production in Chlorella vulgaris

Sajadian, Seyedeh Fatemeh; Morowvat, Mohammad Hossein; Ghasemi, Younes

doi:10.5455/njppp.2018.8.0935625122017

Cited by 16 publications

(9 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Cells were cultivated in both cases under a sufficient nutrient regime and were provided with adequate glucose input in the case of dark cultivation. The response in lipid accumulation by the microalgal cells under autotrophic, mixotrophic and heterotrophic conditions is dependent on the strain and on operational cultivation parameters, such as nutritional factors [ 38 ]. Nannochloropsis gaditana showed similar percentages of lipids on dry weight in autotrophic, mixotrophic and heterotrophic conditions (10.7 – 15.7%) [ 37 ].…”

Section: Resultsmentioning

confidence: 99%

“…1.0 ± 0.2 1.0 ± 0.1 0.9 ± 0.1 1.3 ± 0.1 * Despite the recognized role of diatoms as cell factories for high-value products, few studies have explored their capacity for growth under heterotrophic conditions. Nitzschia laevis represents the most studied case for which the impact of cultivation parameters on the heterotrophic production of EPA has been assessed [25,38] suggesting that this diatom is a good heterotrophic EPA producer. Considering EPA contribution to ω-3 fatty acids determined by GC-MS, the quantitative assessment of EPA production is reported in Table 2.…”

Section: Epa Production and Productivitymentioning

confidence: 99%

See 1 more Smart Citation

Autotrophic vs. Heterotrophic Cultivation of the Marine Diatom Cyclotella cryptica for EPA Production

et al. 2021

View full text Add to dashboard Cite

Recently, the marketable value of ω-3 fatty acid, particularly eicosapentaenoic acid (EPA), increased considering their health effects for human consumption. Microalgae are considered a valuable and “green” source of EPA alternative to fish oils, but considerable efforts are necessary for their exploitation at an industrial level. Due to the high operation costs of photoautotrophic microalgae cultivation, heterotrophic growth represents a promising economic solution. Marine diatoms are the major ecological producers of ω-3 fatty acids. Few species of diatoms are capable to grow in the dark using organic carbon sources. The marine diatom Cyclotella cryptica was cultivated for 14 days under photoautotrophic and heterotrophic conditions to define the effects on growth parameters, lipid production, total fatty acids and EPA content. Photoautotrophic conditions led to a total EPA production of 1.6% of dry weight, 12.2 mg L−1 culture and productivity of 0.9 mg L−1 day−1. The heterotrophy cultures reported a total EPA production of 2.7% of dry cell weight, 18 mg L−1 culture, a productivity of 1.3 mg L−1 day−1, which are promising values in the prospective of improving culture parameters for the biotechnological exploitation of dark cultivation. C. cryptica could be a potential candidate for the heterotrophic production of EPA, also considering its robustness, capacity to resist to bacterial contaminations and plasticity of lipid metabolism.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Epa Production and Productivitymentioning

confidence: 99%

Autotrophic vs. Heterotrophic Cultivation of the Marine Diatom Cyclotella cryptica for EPA Production

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Marquez et al [85] also stated in their studies that Spirulina platensis generated 1.5 times more biomass in mixotrophic growth than photoautotrophic growth. Moreover, higher lipid productivity of neutral lipids was obtained in the stress phase of the mixotrophic-mixotrophic mode, 284 g/kg (52%), and the mixotrophic-heterotrophic mode, 154.3 g/kg (58%) [86], more than the heterotrophic mode, which is 48% of lipid content [87]. This results from the external carbon source that is supplemented even during the stress phase such as the absence of light intensity or nitrogen starvation that influences more generation of unsaturated fatty acid methyl (FAME) and neutral lipid in the mixotrophic mode [86].…”

Section: Attached Microalgal Cultivationmentioning

confidence: 92%

Assuaging Microalgal Harvesting Woes via Attached Growth: A Critical Review to Produce Sustainable Microalgal Feedstock

et al. 2021

View full text Add to dashboard Cite

Third-generation biofuels that are derived from microalgal biomass have gained momentum as a way forward in the sustainable production of biodiesel. Such efforts are propelled by the intention to reduce our dependence on fossil fuels as the primary source of energy. Accordingly, growing microalgal biomass in the form of suspended cultivation has been a conventional technique for the past few decades. To overcome the inevitable harvesting shortcomings arising from the excessive energy and time needed to separate the planktonic microalgal cells from water medium, researchers have started to explore attached microalgal cultivation systems. This cultivation mode permits the ease of harvesting mature microalgal biomass, circumventing the need to employ complex harvesting techniques to single out the cells, and is economically attractive. However, the main bottleneck associated with attached microalgal growth is low biomass production due to the difficulties the microalgal cells have in forming attachment and populating thereafter. In this regard, the current review encompasses the novel techniques adopted to promote attached microalgal growth. The physicochemical effects such as the pH of the culture medium, hydrophobicity, as well as the substratum surface properties and abiotic factors that can determine the fate of exponential growth of attached microalgal cells, are critically reviewed. This review aims to unveil the benefits of an attached microalgal cultivation system as a promising harvesting technique to produce sustainable biodiesel for lasting applications.

show abstract

“…So far, the most common products extracted from heterotrophic microalgal biomass are lipids because these microorganisms usually contain high amounts of intracellular oils with high proportions of high value-added lipids, such as poly-unsaturated fatty acids (PUFA), but low protein and carbohydrates contents [47].…”

Section: Products Extractionmentioning

confidence: 99%

The Role of Heterotrophic Microalgae in Waste Conversion to Biofuels and Bioproducts

et al. 2021

View full text Add to dashboard Cite

In the last few decades, microalgae have attracted attention from the scientific community worldwide, being considered a promising feedstock for renewable energy production, as well as for a wide range of high value-added products such as pigments and poly-unsaturated fatty acids for pharmaceutical, nutraceutical, food, and cosmetic markets. Despite the investments in microalgae biotechnology to date, the major obstacle to its wide commercialization is the high cost of microalgal biomass production and expensive product extraction steps. One way to reduce the microalgae production costs is the use of low-cost feedstock for microalgae production. Some wastes contain organic and inorganic components that may serve as nutrients for algal growth, decreasing the culture media cost and, thus, the overall process costs. Most of the research studies on microalgae waste treatment use autotrophic and mixotrophic microalgae growth. Research on heterotrophic microalgae to treat wastes is still scarce, although this cultivation mode shows several benefits over the others, such as higher organic carbon load tolerance, intracellular products production, and stability in production all year round, regardless of the location and climate. In this review article, the use of heterotrophic microalgae to simultaneously treat wastes and produce high value-added bioproducts and biofuels will be discussed, critically analyzing the most recent research done in this area so far and envisioning the use of this approach to a commercial scale in the near future.

show abstract

Investigation of autotrophic, heterotrophic, and mixotrophic modes of cultivation on lipid and biomass production in Chlorella vulgaris

Cited by 16 publications

References 23 publications

Autotrophic vs. Heterotrophic Cultivation of the Marine Diatom Cyclotella cryptica for EPA Production

Autotrophic vs. Heterotrophic Cultivation of the Marine Diatom Cyclotella cryptica for EPA Production

Assuaging Microalgal Harvesting Woes via Attached Growth: A Critical Review to Produce Sustainable Microalgal Feedstock

The Role of Heterotrophic Microalgae in Waste Conversion to Biofuels and Bioproducts

Contact Info

Product

Resources

About