Investigation of Bacillus licheniformis in the biodegradation of Iranian heavy crude oil: A two-stage sequential approach containing factor-screening and optimization

Khanpour-Alikelayeh, Elham; Partovinia, Ali; Talebi, Ahmad Farhad; Kermanian, Hossein

doi:10.1016/j.ecoenv.2020.111103

Cited by 31 publications

(11 citation statements)

References 46 publications

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“…Before conducting the biodegradation study, it is crucial to optimize the growth parameters for efficient PCB degradation. Various studies have been conducted on optimizing growth conditions to accomplish enhanced biodegradation efficiency ( Khanpour-Alikelayeh et al, 2020 ). Several environmental factors such as the concentration of contaminant, presence of inducers, pH, and temperature, can alter bacterial growth that in turn influences biodegradation efficiency.…”

Section: Resultsmentioning

confidence: 99%

PCB-77 biodegradation potential of biosurfactant producing bacterial isolates recovered from contaminated soil

Sandhu

Paul²,

Proćków³

et al. 2022

Front. Microbiol.

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Polychlorinated biphenyls (PCBs) are persistent organic pollutants widely distributed in the environment and possess deleterious health effects. The main objective of the study was to obtain bacterial isolates from PCB-contaminated soil for enhanced biodegradation of PCB-77. Selective enrichment resulted in the isolation of 33 strains of PCB-contaminated soil nearby Bhilai steel plant, Chhattisgarh, India. Based on the prominent growth using biphenyl as the sole carbon source and the confirmation of its degradation by GC-MS/MS analysis, four isolates were selected for further study. The isolates identified by 16S rRNA gene sequencing were Pseudomonas aeruginosa MAPB-2, Pseudomonas plecoglossicida MAPB-6, Brucella anthropi MAPB-9, and Priestia megaterium MAPB-27. The isolate MAPB-9 showed a degradation of 66.15% biphenyl, while MAPB-2, MAPB-6, and MAPB-27 showed a degradation of 62.06, 57.02, and 56.55%, respectively in 48 h. Additionally, the degradation ability of these strains was enhanced with addition of co-metabolite glucose (0.2%) in the culture medium. Addition of glucose showed 100% degradation of biphenyl by MAPB-9, in 48 h, while MAPB-6, MAPB-2, and MAPB-27 showed 97.1, 67.5, and 53.3% degradation, respectively as analyzed by GC-MS/MS. Furthermore, in the presence of inducer, PCB-77 was found to be 59.89, 30.49, 27.19, and 4.43% degraded by MAPB-6, MAPB-9, MAPB-2, and MAPB-27, respectively in 7 d. The production of biosurfactants that aid in biodegradation process were observed in all the isolates. This was confirmed by ATR-FTIR analysis that showed the presence of major functional groups (CH2, CH3, CH, = CH2, C–O–C, C-O) of the biosurfactant. The biosurfactants were further identified by HPTLC and GC-MS/MS analysis. Present study is the first to report PCB-77 degradation potential of Pseudomonas aeruginosa, B. anthropi, Pseudomonas plecoglossicida, and Priestia megaterium. Similarly, this is the first report on Pseudomonas plecoglossicida and Priestia megaterium for PCB biodegradation. Our results suggest that the above isolates can be used for the biodegradation of biphenyl and PCB-77 in PCB-contaminated soil.

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Section: Resultsmentioning

confidence: 99%

PCB-77 biodegradation potential of biosurfactant producing bacterial isolates recovered from contaminated soil

Sandhu

Paul²,

Proćków³

et al. 2022

Front. Microbiol.

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“…As a nutrients source, a commercial mineral fertilizer (NPK) was used. The treatment design was based on results of a previous experiment on the protective role of PGA in the biodegradation of petroleum-contaminated soil [ 54 ] and the trial-error principle method [ 57 , 58 , 59 ].…”

Section: Methodsmentioning

confidence: 99%

“…Bacillus licheniformis was used in the experiment as the effective species in the biodegradation of crude oil [ 58 ]. The pure colonies of Bacillus licheniformis NCBI 1402, which was used as an efficient PGA producer, were incubated in a broth culture medium (sodium glutamate 5 g, glucose 7 g, sodium citrate 0.7 g, ammonium sulfate 0.7 g, sodium nitrate 0.7 g, yeast extract 1.6 g, peptone 1.6 g, zinc sulfate 0.07 g, and dipotassium phosphate 0.07 g) for 48 h. A colony forming unit (CFU) of the samples was enumerated by a serial dilution technique: samples were serially diluted up to 10 8 dilutions and plated into starch-mineral salt agar medium, composed of 10 g starch (soluble), 1.8 g K 2 HPO 4 , 4.0 g NH 4 Cl, 0.2 g MgSO 4 ·7H 2 O, 0.1 g NaCl, 0.01 g FeSO 4 ·7H 2 O, and 15 g agar in 1 L of distilled water.…”

Section: Methodsmentioning

confidence: 99%

Hydrocarbon Degradation and Microbial Survival Improvement in Response to γ-Polyglutamic Acid Application

Zając

Fabiańska

Jedrszczyk

et al. 2022

IJERPH

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To improve the environmental sustainability of cleanup activities of contaminated sites there is a need to develop technologies that minimize soil and habitat disturbances. Cleanup technologies, such as bioremediation, are based on biological products and processes, and they are important for the future of our planet. We studied the potential of γ-poly glutamic acid (PGA) as a natural component of biofilm produced by Bacillus sp. to be used for the decomposition of petroleum products, such as heavy naphtha (N), lubricating oil (O), and grease (G). The study aimed to assess the impact of the use of different concentrations of PGA on the degradation process of various fractions of petroleum hydrocarbons (PH) and its effect on bacterial population growth in harsh conditions of PH contamination. In laboratory conditions, four treatments of PGA with each of the petroleum products (N, O, and G) were tested: PGA0 (reference), PGA1 (1% PGA), PGA1B (1% PGA with Bacillus licheniformis), and PGA10 (10% PGA). After 7, 28, 56, and 112 days of the experiment, the percentage yield extraction, hydrocarbon mass loss, geochemical ratios, pH, electrical conductivity, and microorganisms survival were determined. We observed an increase in PH removal, reflected as a higher amount of extraction yield (growing with time and reaching about 11% in G) and loss of hydrocarbon mass (about 4% in O and G) in all treatments of the PGA compared to the reference. The positive degradation impact was intensive until around day 60. The PH removal stimulation by PGA was also reflected by changes in the values of geochemical ratios, which indicated that the highest rate of degradation was at the initial stage of the process. In general, for the stimulation of PH removal, using a lower (1%) concentration of PGA resulted in better performance than a higher concentration (10%). The PH removal facilitated by PGA is related to the anionic homopoliamid structure of the molecule and its action as a surfactant, which leads to the formation of micelles and the gradual release of PH absorbed in the zeolite carrier. Moreover, the protective properties of PGA against the extinction of bacteria under high concentrations of PH were identified. Generally, the γ-PGA biopolymer helps to degrade the hydrocarbon pollutants and stabilize the environment suitable for microbial degraders development.

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“…Biosurfactant produced from Bacillus subtilis strain Al-Dhabi-130 and Staphylococcus sp. CO100 degraded crude oil and reduced surface tension (Dhabi et al 2020; Alnuaimi et al 2020 ; Annie et al 2020 ; Deivakumari et al 2020 ; Khanpour-Alikelayeh et al 2020 ; Maamar et al 2020 ; Ławniczak et al 2020 ).…”

Section: Existing and Emerging Remediation Approach For Crude Oil Pollutionmentioning

confidence: 99%

“…Moj-3449 Biodegradation Long chain n-alkanes 44.26% w/v – – Rhamnolipids produced from Pseudomonas aeruginosa PTCC1340 – Heavy metals in crude oil – – Kholghi et al ( 2020 ) Biosurfactant produced from Bacillus subtilis strain Al-Dhabi-130 Biodegradation Al-Dhabi et al ( 2020 ) Staphylococcus sp. CO100 strain with its biosurfactant Biodegradation Crude oil aliphatic hydrocarbons 72% – – Bacillus megaterium Biodegradation Crude oil – – Alnuaimi et al ( 2020 ) Pseudomonas putida immobilized on coconut coir pith Biodegradation Total petroleum hydrocarbons – – Annie et al ( 2020 ) Pseudomonas aeruginosa Biodegradation Crude oil 63.38% 60 days Deivakumari et al ( 2020 ) Bacillus licheniformis Biodegradation Heavy crude oil 15–66% 14 days Khanpour-Alikelayeh et al ( 2020 ) Pyrolytic treatment Volatilization Light oil – – …”

Section: Introductionmentioning

confidence: 99%

Crude oil exploration in Africa: socio-economic implications, environmental impacts, and mitigation strategies

et al. 2021

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Crude oil exploration is a source of significant revenue in Africa via trade and investment since its discovery in the mid-19th Century. Crude oil has bolstered the continent’s economy and improved the wellbeing of the citizenry. Historically, Africa has suffered from conflicts due to uneven redistribution of crude oil revenue and severe environmental pollution. Advancements in geophysical survey techniques, such as magnetic and gravity methods, to seismic methods, have made the commercial exploration of crude oil possible for some other countries in Africa apart from Nigeria, Angola, Algeria, Libya, and Egypt. The occurrence of organic-rich, oil-prone Type I, II, and mixed II/III kerogens in sedimentary basins and entrapment within reservoir rocks with intrinsic petrophysical properties are majorly responsible for the large deposits of hydrocarbon in Africa. The unethical practices by some multinational oil corporations have resulted in social movements against them by host communities and human rights groups. The unscrupulous diversion of public funds, award of oil blocks, and production rights to certain individuals have impaired economic growth in Africa. The over-dependence on crude oil revenues has caused the economic recession in oil-producing countries due to plummeting oil prices and global pandemic. Most host communities of crude oil deposits suffer from a lack of infrastructure, arable soils, clean water, and their functioning capabilities are violated by crude oil exploratory activities, without adequate compensations and remedial actions taken by oil companies and the government. Thus, this review examines crude oil exploration in Africa and provides insight into the environmental and socio-economic implications of crude oil exploration in Africa. Furthermore, this report highlights some recommendations that may ensure ethical and sustainable practices toward minimizing negative impacts and improving the quality of life in affected communities.

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Investigation of Bacillus licheniformis in the biodegradation of Iranian heavy crude oil: A two-stage sequential approach containing factor-screening and optimization

Cited by 31 publications

References 46 publications

PCB-77 biodegradation potential of biosurfactant producing bacterial isolates recovered from contaminated soil

PCB-77 biodegradation potential of biosurfactant producing bacterial isolates recovered from contaminated soil

Hydrocarbon Degradation and Microbial Survival Improvement in Response to γ-Polyglutamic Acid Application

Crude oil exploration in Africa: socio-economic implications, environmental impacts, and mitigation strategies

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