Investigation of Biological Activity of Nanoparticles Using Cell Lines

Jasti, Tejaswi; Anirudh, Kaligotla Venkata Subrahmanya; Majeti, Lalitha Rishika; Kotagiri, Divya; Shaik, Khasim Beebi; Chaitanya, K. V.

doi:10.1007/978-981-15-1702-0_7

Cited by 2 publications

(2 citation statements)

References 84 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…When nanoparticles are suspended in any medium, the properties of the material get modified to a great extent [ 20 ]. Due to this nanoparticles’ property, we analyzed by ATR-FTIR the spectral properties of the pure powder and the one suspended in 0.9% NaCl SiO 2 -NPs.…”

Section: Resultsmentioning

confidence: 99%

Astrocytes Are More Vulnerable than Neurons to Silicon Dioxide Nanoparticle Toxicity in Vitro

Limón-Pacheco

Jiménez-Barrios

Déciga-Alcaraz

et al. 2020

Toxics

View full text Add to dashboard Cite

Some studies have shown that silicon dioxide nanoparticles (SiO2-NPs) can reach different regions of the brain and cause toxicity; however, the consequences of SiO2-NPs exposure on the diverse brain cell lineages is limited. We aimed to investigate the neurotoxic effects of SiO2-NP (0–100 µg/mL) on rat astrocyte-rich cultures or neuron-rich cultures using scanning electron microscopy, Attenuated Total Reflection-Fourier Transform Infrared spectroscopy (ATR-FTIR), FTIR microspectroscopy mapping (IQ mapping), and cell viability tests. SiO2-NPs were amorphous particles and aggregated in saline and culture media. Both astrocytes and neurons treated with SiO2-NPs showed alterations in cell morphology and changes in the IR spectral regions corresponding to nucleic acids, proteins, and lipids. The analysis by the second derivative revealed a significant decrease in the signal of the amide I (α-helix, parallel β-strand, and random coil) at the concentration of 10 µg/mL in astrocytes but not in neurons. IQ mapping confirmed changes in nucleic acids, proteins, and lipids in astrocytes; cell death was higher in astrocytes than in neurons (10–100 µg/mL). We conclude that astrocytes were more vulnerable than neurons to SiO2-NPs toxicity. Therefore, the evaluation of human exposure to SiO2-NPs and possible neurotoxic effects must be followed up.

show abstract

Section: Resultsmentioning

confidence: 99%

Astrocytes Are More Vulnerable than Neurons to Silicon Dioxide Nanoparticle Toxicity in Vitro

Limón-Pacheco

Jiménez-Barrios

Déciga-Alcaraz

et al. 2020

Toxics

View full text Add to dashboard Cite

show abstract

“…Previous research has reported that toxicity observed by low fertility is also related to NPs size, which is the main feature that allows NPs to easily permeate the cell membrane and induce a biological response [ 45 ]. Gorth et al [ 35 ] found that smaller NPs (20–30 nm) have a less toxic effect compared to those of 100 nm and 500–1200 nm because structurally, they do not accumulate within vital organs and thus do not cause a reduction in internal dose administration; however, they also pointed out a higher accumulation of smaller NPs at non-vital sites.…”

Section: Discussionmentioning

confidence: 99%

Fertility and Iron Bioaccumulation in Drosophila melanogaster Fed with Magnetite Nanoparticles Using a Validated Method

et al. 2021

View full text Add to dashboard Cite

Research on nanomaterial exposure-related health risks is still quite limited; this includes standardizing methods for measuring metals in living organisms. Thus, this study validated an atomic absorption spectrophotometry method to determine fertility and bioaccumulated iron content in Drosophila melanogaster flies after feeding them magnetite nanoparticles (Fe3O4NPs) dosed in a culture medium (100, 250, 500, and 1000 mg kg−1). Some NPs were also coated with chitosan to compare iron assimilation. Considering both accuracy and precision, results showed the method was optimal for concentrations greater than 20 mg L−1. Recovery values were considered optimum within the 95–105% range. Regarding fertility, offspring for each coated and non-coated NPs concentration decreased in relation to the control group. Flies exposed to 100 mg L−1 of coated NPs presented the lowest fertility level and highest bioaccumulation factor. Despite an association between iron bioaccumulation and NPs concentration, the 500 mg L−1 dose of coated and non-coated NPs showed similar iron concentrations to those of the control group. Thus, Drosophila flies’ fertility decreased after NPs exposure, while iron bioaccumulation was related to NPs concentration and coating. We determined this method can overcome sample limitations and biological matrix-associated heterogeneity, thus allowing for bioaccumulated iron detection regardless of exposure to coated or non-coated magnetite NPs, meaning this protocol could be applicable with any type of iron NPs.

show abstract

Investigation of Biological Activity of Nanoparticles Using Cell Lines

Cited by 2 publications

References 84 publications

Astrocytes Are More Vulnerable than Neurons to Silicon Dioxide Nanoparticle Toxicity in Vitro

Astrocytes Are More Vulnerable than Neurons to Silicon Dioxide Nanoparticle Toxicity in Vitro

Fertility and Iron Bioaccumulation in Drosophila melanogaster Fed with Magnetite Nanoparticles Using a Validated Method

Contact Info

Product

Resources

About