Investigation of brucellosis in cattle and buffaloes by conventional and molecular assays

Kaur, Parvinder; Sharma, N S; Arora, Anil; Deepti,

doi:10.18805/ijar.b-3375

Cited by 7 publications

(5 citation statements)

References 22 publications

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“…Isolation of organism from clinical specimen though considered gold standard, is time consuming and laborious. Due to the limitations concerned with serological and bacteriological methods, molecular methods like PCR is being used as an alternative for detection of pathogens (Mothershed et al,2006) directly in clinical specimens (Kanani et al, 2008;Kaur et al,2017) or to confirm the cultural isolates.…”

Section: Resultsmentioning

confidence: 99%

Single-tube duplex-PCR for specific detection and differentiation of Brucella abortus S19 vaccine strain from other Brucella spp

Das

Kumar

Chakravarti

et al. 2018

IJAR

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A simple single-tube duplex-PCR assay was optimized for rapid and sensitive detection and differentiation of Brucella abortus (B.abortus) S19 vaccine strain from other Brucella spp.(B.abortus 544,B abortus S99,B.melitensis 16M,B.suis).This assay was optimized using two primer pairs that were designed, one targeting genus specific multicopy IS711 and another eryC for the development of the duplex-PCR assay. Specificity of the assay was assessed using DNA templates from various Brucella species (B.abortus, B.melitensis, B.suis) and non Brucella bacteria like Staphylococcus aureus, Proteus vulgaris, Pasteurella multocida, Pseudomonas aeruginosa, Citrobacter freundii, E. coli, Salmonella enteritidis, Campylobacter jejuni and Listeria monocytogenes. The assay was also evaluated on spiked milk samples with known B.abortus cultures. The assay was able to detect up to 2.47 x 103CFU of B. abortus S99 organism in spiked milk sample. The assay was further validated in 53 clinical specimens (aborted foetal stomach content). Results confirm that Single tube duplex-PCR assay was useful for early and rapid detection and differentiation of B. abortus vaccine strain S19 from other Brucella spp in milk as well as in clinical specimen.

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Section: Resultsmentioning

confidence: 99%

Single-tube duplex-PCR for specific detection and differentiation of Brucella abortus S19 vaccine strain from other Brucella spp

Das

Kumar

Chakravarti

et al. 2018

IJAR

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“…The transmission and spread of cattle brucellosis are favoured in areas practising open grazing in which cattle freely interact within a herd and between herds. The spread is realized through shared grazing areas, shared bulls (if natural breeding is practised), shared water sources, contaminated and contaminating aborted materials, vaginal discharges and manure (Aparicio, 2013; Kaur et al., 2018; Tekle et al., 2019). With regard to our findings in this study, however, the high proportion of contaminated herds in open grazing areas may not be due to shared grazing areas as farms are predominantly fenced, but it may be explained by shared water sources and shared bulls.…”

Section: Discussionmentioning

confidence: 99%

Sero‐prevalence and risk factors of Brucella presence in farm bulk milk from open and zero grazing cattle production systems in Rwanda

Djangwani

Abong

Njue

et al. 2021

Veterinary Medicine & Sci

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Background Animal and human brucelloses have been reported in Rwanda, human brucellosis being linked to drinking inadequately heat‐treated milk. However, information on Brucella detection and prevalence in milk produced in Rwanda is limited. Objectives To determine the sero‐prevalence and risk factors of Brucella in farm bulk milk from zero and open grazing cattle production systems in Rwanda. Methods A total of 330 farm bulk milk samples were collected from 198 zero grazing farms and 132 open grazing farms in a cross‐sectional study in Rwanda. Sero‐prevalence of Brucella in milk was analysed using indirect enzyme‐linked immunosorbent assay. A questionnaire was administered to farmers to determine the risk factors of milk contamination with Brucella. Results Anti‐Brucella antibodies were prevalent in 19.7% (95% confidence interval (CI), 15.5–24.4) of the 330 collected farm bulk milk. Sero‐prevalence was significantly higher (p < 0.05) in open grazing farms (37.9% [50/132]) than in zero grazing farms (7.6% [15/198]). Practising open grazing system (odds ratio, OR = 69.5; 95% CI = 1.6–3033.6), history of abortion (OR = 19.5; 95% CI = 8.1–46.8) and placenta retention (OR = 4.2; 95% CI = 1.7–10.3) were the significant risk factors for the presence of anti‐Brucella antibodies in milk. Conclusion Notably, more than a third of farm bulk milk from open grazing farms in Rwanda contains Brucella antibodies. Considering the zoonotic nature of Brucella, there is a need to reinforce brucellosis control programs in the country.

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“…Genomic DNA was extracted from the isolates using a QIAamp DNA Mini Kit (Qiagen, USA). The multiplex PCR (Bruce-ladder) using eight primers could differentiate most Brucella species, including marine mammals and the vaccine strains of B. abortus S19, B. abortus RB51 and B. melitensis Rev.1 in a single reaction (Kaur et al, 2018;López-Goñi et al, 2008). The Bruce-ladder PCR was defined as 0.32 pmol/µL of each primer, 0.05 U Takara Ex Ta® (Takara BIO INC, Japan), 0.25 mmol -dNTP and 1X Takara Ex buffer (Takara BIO INC, Japan) to a final volume of 25 µL with 3 µL of this DNA at 10-100 ng /µL.…”

Section: Genus-specific Pcr: Multiplex Pcr (Bruce-ladder)mentioning

confidence: 99%

The Seroprevalence Rates and Approach Management of Brucellosis in Thailand

Kanitpun

Ekgatat

Arunvipas

2022

IJAR

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Background: Brucellosis is an important re-emerging zoonosis with worldwide distribution. In Thailand, endemic bovine and caprine brucellosis is a serious public health threat. Brucellosis in cattle and buffalo is primarily due to Brucella abortus while Brucella melitensis predominates in sheep and goats. The main factors maintaining the disease in humans and animals are unhygienic food habits, poor animal husbandry processes, lack of awareness and ineffective surveillance systems. Therefore, prevention of human brucellosis would depend on controlling the disease in livestock. The current study aimed to study the epidemiological situation to develop an effective control strategy. Methods: The estimated seropositivity rates at the herd and animal levels were compiled during 2009-2018. The sera were submitted to the National Institute of Animal Health (NIAH) and the Regional Veterinary Research and Development Centers (VRDCs), where serological tests were performed. The testing used screening based on the Rose Bengal test (RBT) followed by confirmatory tests, the complement fixation test (CFT) and the indirect enzyme-linked immunosorbent assay (I-ELISA). The data were collected, the proportion of the estimated seropositivity rate was analyzed. The seropositive animals were followed up and specimens were collected for isolation and PCR identification. Result: The results of the accumulated seropositivity rate of the brucellosis of dairy cattle, beef cattle, buffaloes, goats and sheep at the herd level from 2009 to 2018 was 7.12% (95% CI: 6.99-7.26) and the animal level was 0.92% (95% CI: 0.91-0.93). The species identification presented Brucella abortus five isolates, whereas Brucella melitensis presented 31 isolates from the total of 36 isolates. This information of the serological status and Brucella spp., including diagnostics patterns could be used as base data for better managing the prevention and control measures of brucellosis in Thailand and other countries.

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Investigation of brucellosis in cattle and buffaloes by conventional and molecular assays

Cited by 7 publications

References 22 publications

Single-tube duplex-PCR for specific detection and differentiation of Brucella abortus S19 vaccine strain from other Brucella spp

Single-tube duplex-PCR for specific detection and differentiation of Brucella abortus S19 vaccine strain from other Brucella spp

Sero‐prevalence and risk factors of Brucella presence in farm bulk milk from open and zero grazing cattle production systems in Rwanda

The Seroprevalence Rates and Approach Management of Brucellosis in Thailand

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